PRINT

Lamin B1 Peptide 1

Antibody: CPTC-LMNB1-1
Background

Catalog Number:

CPTC-LMNB1-1

Target Antigen:

Lamin B1 Peptide 1

Isotype:

IgG

Species:

Rabbit Recombinant Cloned Antibody

Last Updated:

03/20/2024

Antigen Recognition(s):

Peptide, Endogenous, Phosphorylation

External Links
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (296.6 KB)

CPTC-LMNB1-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC NCI60
Download Immuno-histochemistry of CPTC-LMNB1-1 for NCI60 Cell Line Array with titer 1:50 0=NEGATIVE 1=WEAK(red) 2=MODERATE(blue) 3=STRONG(green) (71.6 KB)

CPTC-LMNB1-1 IHC NCI60

Result: Positive

Immuno-histochemistry of CPTC-LMNB1-1 for NCI60 Cell Line Array with titer 1:50
0=NEGATIVE
1=WEAK(red)
2=MODERATE(blue)
3=STRONG(green)


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Micro-Array(TMA) core of pancreatic cancer showing nuclear staining using Antibody CPTC-LMNB1-1. Titer: 1:50 Click image to enlarge

CPTC-LMNB1-1 IHC Tissue

Result: Positive

Tissue Micro-Array(TMA) core of pancreatic cancer showing nuclear staining using Antibody CPTC-LMNB1-1. Titer: 1:50


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-LMNB1-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5902) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from cell lysate for teh double phosphorylated peptide AGGPTpTPLpSPTR. Click image to enlarge

CPTC-LMNB1-1 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-LMNB1-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5902)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from cell lysate for teh double phosphorylated peptide AGGPTpTPLpSPTR.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-LMNB1-1 as primary antibody against cell lysates HeLa, MCF10A, and LCL57. Samples from each cell line were irradiated with 10 Gy as shown in ‘+’ indicated lanes. Samples from each non-irradiated cell line were treated with alkaline phosphatase enzyme as shown in ‘-‘ indicated lanes. Molecular weight standards are included for each cell line. Click image to enlarge

CPTC-LMNB1-1 automated western blot (cell lysate)

Result: Negative

Automated western blot using CPTC-LMNB1-1 as primary antibody against cell lysates HeLa, MCF10A, and LCL57. Samples from each cell line were irradiated with 10 Gy as shown in ‘+’ indicated lanes. Samples from each non-irradiated cell line were treated with alkaline phosphatase enzyme as shown in ‘-‘ indicated lanes. Molecular weight standards are included for each cell line.


Characterization SOP Files

[top]

Antibody: CPTC-LMNB1-2
Background

Catalog Number:

CPTC-LMNB1-2

Target Antigen:

Lamin B1 Peptide 1

Isotype:

IgG

Species:

Rabbit Recombinant Cloned Antibody

Last Updated:

03/20/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous, Phosphorylation

External Links
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org). (418.2 KB)

CPTC-LMNB1-2 evaluation by the Human Protein Atlas

Result: Positive

This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  IHC Tissue

CTPC-LMNB1-2 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-LMNB1-2 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5901) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from cell lysate for teh double phosphorylated peptide AGGPTpTPLpSPTR. Click image to enlarge

CPTC-LMNB1-2 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-LMNB1-2 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5901)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from cell lysate for teh double phosphorylated peptide AGGPTpTPLpSPTR.


Characterization SOP Files

  Immunofluorescence
Click to enlarge image Immunofluorescence staining of human cell line HeLa with CPTC-LMNB1-2 Ab shows localization to the nucleus. Click image to enlarge

CPTC-LMNB1-2

Result: Positive

Immunofluorescence staining of human cell line HeLa with CPTC-LMNB1-2 Ab shows localization to the nucleus.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-LMNB1-2 as primary antibody against human recombinant Lamin B1 protein (lane 2). Also included are molecular weight standards (lane 1). Click image to enlarge

CPTC-LMNB1-2 western blot (recombinant protein)

Result: Presumed Positive (with additional bands)

Western Blot using CPTC-LMNB1-2 as primary antibody against human recombinant Lamin B1 protein (lane 2). Also included are molecular weight standards (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-LMNB1-2 as primary antibody against cell lysates LCL57 (lane 2), HeLa (lane 3) and MCF10A (lane 4). Also included are molecular weight standards (lane 1). Click image to enlarge

CPTC-LMNB1-2 western blot (cell lysates)

Result: Positive

Western Blot using CPTC-LMNB1-2 as primary antibody against cell lysates LCL57 (lane 2), HeLa (lane 3) and MCF10A (lane 4). Also included are molecular weight standards (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-LMNB1-2 as primary antibody against cell lysates LCL57 (lane 2), HeLa (lane 3) and MCF10A (lane 4). Also included are molecular weight standards (lane 1). Click image to enlarge

CPTC-LMNB1-2 automated western blot (cell lysate)

Result: Negative

Western Blot using CPTC-LMNB1-2 as primary antibody against cell lysates LCL57 (lane 2), HeLa (lane 3) and MCF10A (lane 4). Also included are molecular weight standards (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Single cell western blot using CPTC-LMNB1-2 as a primary antibody against cell lysates. Relative expression of total LMNB1 in HeLa, MCF10A, and LCL57 cells (A). Percentage of cells that express LMNB1 (B). Average expression of LMNB1 protein per cell (C). All data is normalized to β-tubulin expression. Click image to enlarge

CPTC-LMNB1-2 Single Cell Western Blot (Cell Lysate)

Result: Positive

Single cell western blot using CPTC-LMNB1-2 as a primary antibody against cell lysates. Relative expression of total LMNB1 in HeLa, MCF10A, and LCL57 cells (A). Percentage of cells that express LMNB1 (B). Average expression of LMNB1 protein per cell (C). All data is normalized to β-tubulin expression.


Characterization SOP Files

[top]

Antigen: Lamin B1 Peptide 1
Background

NCI Identification Number:

00294

Antigen Name:

Lamin B1 Peptide 1

CPTC Name:

CPTC-LMNB1 Peptide 1

Aliases:

Lamin B1; Lamin-B1; LMN2; LMNB; ADLD; LMN

Function:

This gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants.LMNB1 (Lamin B1) is a Protein Coding gene. Diseases associated with LMNB1 include Leukodystrophy, Demyelinating, Adult-Onset, Autosomal Dominant and Leukodystrophy. Among its related pathways are Apoptosis and survival Caspase cascade and DNA Damage/Telomere Stress Induced Senescence. Gene Ontology (GO) annotations related to this gene include structural molecule activity and phospholipase binding. An important paralog of this gene is LMNB2.Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.

Chromosomal Localization:

5q23.2

Accession Number:

NP_005564.1

UniProt Accession Number:

P20700

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

CAGGPTT(ph)PLS(ph)PTR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1430

Last Updated:

08/21/2020

Links

Characterization Data

SOPs

No SOPs available.

[top]

Don't have Adobe Reader™?

Get it for free at Adobe.com

  [top]