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Ladybird Homeobox 1 Peptide 2

Antibody: CPTC-LBX1-2
Background

Catalog Number:

CPTC-LBX1-2

Target Antigen:

Ladybird Homeobox 1 Peptide 2

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

02/06/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length

Thematic Panel(s):

Epithelial Mesenchymal Transition (EMT)

Purchase
Publications
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (339.7 KB)

CPTC-LBX1-2 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IP
Download Immuno-precipitation data obtained incubating CPTC-LBX1-2 and the recombinant domain of LBX1 (aa1-281, expected, MW ~31KDa) (143.8 KB)

CPTC-LBX1-2 IPMS

Result: Positive

Immuno-precipitation data obtained incubating CPTC-LBX1-2 and the recombinant domain of LBX1 (aa1-281, expected, MW ~31KDa)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-LBX1-2 as primary antibody against full length LBX1 protein. Click image to enlarge

CPTC-LBX1-2 Indirect ELISA

Result: Positive

Indirect ELISA using CPTC-LBX1-2 as primary antibody against full length LBX1 protein.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-LBX1-2 as primary antibody against recombinant LBX1 protein (lane 2) with expected MW of 33 KDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-LBX1-2 Western Blot (Recombinant Protein)

Result: Presumed Positive (with additional bands)

Western Blot using CPTC-LBX1-2 as primary antibody against recombinant LBX1 protein (lane 2) with expected MW of 33 KDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-LBX1-2 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 30.2 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-LBX1-2 Endogenous Western Blot

Result: Negative

Western Blot using CPTC-LBX1-2 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 30.2 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated Western Blot using CPTC-LBX1-2 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 30.2 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-LBX1-2 Endogenous Automated Western Blot

Result: Negative

Automated Western Blot using CPTC-LBX1-2 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 30.2 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1).


Characterization SOP Files

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Antibody: CPTC-LBX1-3
Background

Catalog Number:

CPTC-LBX1-3

RRID:

AB_2827840

Target Antigen:

Ladybird Homeobox 1 Peptide 2

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

02/06/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length

Thematic Panel(s):

Epithelial Mesenchymal Transition (EMT)

Purchase
Publications
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (339.5 KB)

CPTC-LBX1-3 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IP
Download Immuno-precipitation data obtained incubating CPTC-LBX1-3 and the recombinant domain of LBX1 (aa1-281, expected, MW ~31KDa) (147.6 KB)

CPTC-LBX1-3 IPMS

Result: Positive

Immuno-precipitation data obtained incubating CPTC-LBX1-3 and the recombinant domain of LBX1 (aa1-281, expected, MW ~31KDa)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-LBX1-3 as primary antibody against full length LBX1 protein. Click image to enlarge

CPTC-LBX1-3 Indirect ELISA

Result: Positive

Indirect ELISA using CPTC-LBX1-3 as primary antibody against full length LBX1 protein.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-LBX1-3 as primary antibody against recombinant LBX1 protein (lane 2) with expected MW of 33 KDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-LBX1-3 Western Blot (Recombinant Protein)

Result: Presumed Positive (with additional bands)

Western Blot using CPTC-LBX1-3 as primary antibody against recombinant LBX1 protein (lane 2) with expected MW of 33 KDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-LBX1-3 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 30.2 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-LBX1-3 Endogenous Western Blot

Result: Negative

Western Blot using CPTC-LBX1-3 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 30.2 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated Western Blot using CPTC-LBX1-3 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 30.2 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-LBX1-3 Endogenous Automated Western Blot

Result: Negative

Automated Western Blot using CPTC-LBX1-3 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 30.2 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1).


Characterization SOP Files

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Antigen: Ladybird Homeobox 1 Peptide 2
Background

NCI Identification Number:

00076

Antigen Name:

Ladybird Homeobox 1 Peptide 2

CPTC Name:

CPTC-LBX1 Peptide 2

Aliases:

LBX1H; Ladybird Homeobox Protein Homolog 1; Ladybird Homeobox Homolog 1 (Drosophila); HPX-6; HPX6; homeobox; Lady Bird-Like Homeobox; Ladybird Homeobox Homolog 1; Transcription Factor LBX1; transcription Factor Similar To D. Melanogaster Homeodomain Protein Lady Bird Late

Function:

This gene and the orthologous mouse gene were found by their homology to the Drosophila lady bird early and late homeobox genes. In the mouse, this gene is a key regulator of muscle precursor cell migration and is required for the acquisition of dorsal identities of forelimb muscles.

LBX1 (ladybird homeobox 1) is a protein-coding gene. Diseases associated with LBX1 include leri weill dyschondrosteosis, and adenoid cystic carcinoma. GO annotations related to this gene include transcription regulatory region sequence-specific DNA binding and sequence-specific DNA binding transcription factor activity. An important paralog of this gene is DLX2.

Transcription factor required for the development of GABAergic interneurons in the dorsal horn of the spinal cord and migration and further development of hypaxial muscle precursor cells for limb muscles, diaphragm and hypoglossal cord

Chromosomal Localization:

10q24

Accession Number:

NP_006553.2

UniProt Accession Number:

P52954

DNA Source:

The NCI acknowledges the generous advice and gene clones provided by Robert A. Weinberg at the Ludwig Center for Cancer Research of the Massachusetts Institute of Technology. These reagents have been provided by the Division of Cancer Treatment and Diagnosis (DCTD) of the National Cancer Institute (NCI) using funding of the American Recovery and Reinvestment Act of 2009

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

cLEELASKTFKGLEVSVLQA

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

2200

Last Updated:

09/16/2019

Links

Characterization Data

SOPs

No SOPs available.

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