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Cellular Retinoic Acid Binding Protein 2 Peptide 2

Antibody: CPTC-CRABP2-2
Background

Catalog Number:

CPTC-CRABP2-2

Target Antigen:

Cellular Retinoic Acid Binding Protein 2 Peptide 2

Isotype:

IgG

Species:

Rabbit Recombinant Cloned Antibody

Last Updated:

02/02/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

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Characterization Data [Compare Characterization Data]
  Affinity Measurement by Biolayer Interferometry
Click to enlarge image The affinity and binding kinetics of CPTC-CRABP2-2 and CRABP2 recombinant protein were measured using biolayer interferometry. Human CRABP2 protein (His Tag) (Cat# 11203-H07E, Sino Biological) was buffer exchanged and then covalently immobilized onto amine-reactive second-generation, AR2G, biosensors. CPTC-CRABP2-2 antibody at 256 nM, 64 nM, 16 nM, 4 nM and 1 nM, was used as analyte. KD, Ka, and Kd parameters were determined with Octet Data Analysis HT evaluation software v11.1.3.50 using a 1:1 binding model with global fitting. Click image to enlarge

CPTC-CRABP2-2 Affinity and Kinetics

Result: Positive

The affinity and binding kinetics of CPTC-CRABP2-2 and CRABP2 recombinant protein were measured using biolayer interferometry. Human CRABP2 protein (His Tag) (Cat# 11203-H07E, Sino Biological) was buffer exchanged and then covalently immobilized onto amine-reactive second-generation, AR2G, biosensors. CPTC-CRABP2-2 antibody at 256 nM, 64 nM, 16 nM, 4 nM and 1 nM, was used as analyte. KD, Ka, and Kd parameters were determined with Octet Data Analysis HT evaluation software v11.1.3.50 using a 1:1 binding model with global fitting.


Characterization SOP Files

  Affinity Measurement by SPR
Click to enlarge image The affinity and binding kinetics of CPTC-CRABP2-2 and CRABP2 recombinant protein were measured using surface plasmon resonance. Human CRABP2 protein (His Tag) (Cat# 11203-H07E, Sino Biological) was buffer exchanged and then covalently immobilized onto a Series S CM5 chip. CPTC-CRABP2-2 antibody at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM and 1 nM, was used as analyte. KD, Ka and Kd parameters were determined with Biacore T200 Evaluation Software V3.2 using a 1:2 binding model with global fitting. Click image to enlarge

CPTC-CRABP2-2 Affinity and Kinetics

Result: Positive

The affinity and binding kinetics of CPTC-CRABP2-2 and CRABP2 recombinant protein were measured using surface plasmon resonance. Human CRABP2 protein (His Tag) (Cat# 11203-H07E, Sino Biological) was buffer exchanged and then covalently immobilized onto a Series S CM5 chip. CPTC-CRABP2-2 antibody at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM and 1 nM, was used as analyte. KD, Ka and Kd parameters were determined with Biacore T200 Evaluation Software V3.2 using a 1:2 binding model with global fitting.


Characterization SOP Files

  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (517.1 KB)

CPTC-CRABP2-2 evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Micro-Array (TMA) core of breast showing cytoplasmic staining using Antibody CPTC-CRABP2-2. Titer: 1:1000 Click image to enlarge

CPTC-CRABP2-2 IHC Tissue

Result: Presumed Positive

Tissue Micro-Array (TMA) core of breast showing cytoplasmic staining using Antibody CPTC-CRABP2-2. Titer: 1:1000


Characterization SOP Files

  IP

CPTC-CRABP2-2 IP-WB

Result: Negative

Antibody CPTC-CRABP2-2 was tested in IP-WB in Cell lines OVCAR-8 and OVCAR-3. The antibody is not suitable for this application.


Characterization SOP Files

  Immuno-MRM
Click to enlarge image iMRM screening of CPTC-CRABP2-2 against synthetic peptide QEGDTFYIK (Cellular Retinoic Acid Binding Protein 2 Peptide 2) Data provided by the Carr Lab, Broad Institute https://www.broadinstitute.org/proteomics/protocols Click image to enlarge

CPTC-CRABP2-2 iMRM (synthetic peptide)

Result: Positive

iMRM screening of CPTC-CRABP2-2 against synthetic peptide QEGDTFYIK (Cellular Retinoic Acid Binding Protein 2 Peptide 2)

Data provided by the Carr Lab, Broad Institute
https://www.broadinstitute.org/proteomics/protocols


Characterization SOP Files

  Immunofluorescence

CPTC-CRABP2-2

Result: Negative

Immunofluorescence staining of human cell line OVCAR-3 with CPTC-CRABP2-2 Ab shows no localization of CRABP2 protein.


Characterization SOP Files

  Immunofluorescence
Click to enlarge image Immunofluorescence staining of human cell line OVCAR-3 using CPTC-CRABP2-2 as primary antibody. CRABP2 protein expression shows localization in the cytoplasm (green). Click image to enlarge

CPTC-CRABP2-2 Immunofluorescence

Result: Positive

Immunofluorescence staining of human cell line OVCAR-3 using CPTC-CRABP2-2 as primary antibody. CRABP2 protein expression shows localization in the cytoplasm (green).


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (517.1 KB)

CPTC-CRABP2-2 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-CRABP2-2 as primary antibody against CRABP2 recombinant protein. Click image to enlarge

CPTC-CRABP2-2 Indirect ELISA

Result: Positive

Indirect ELISA using CPTC-CRABP2-2 as primary antibody against CRABP2 recombinant protein.


Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-CRABP2-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-CRABP2-2 NCI 60 Protein Array

Result: Positive

Protein Array in which CPTC-CRABP2-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-CRABP2-2 as primary antibody against CRABP2 recombinant protein. Molecular weight standards are included. Click image to enlarge

CPTC-CRABP2-2 Western Blot (Recombinant Protein)

Result: Positive

Western blot using CPTC-CRABP2-2 as primary antibody against CRABP2 recombinant protein. Molecular weight standards are included.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-CRABP2-2 as primary antibody against cell lysates and supernatants of cell lines OVCAR-3, OVCAR-4, OVAR-8 and SK-OV-3. Expected molecular weight is 15.6 KDa. Positive for OVCAR-3 cell lysates. Molecular weight standards are also included. Click image to enlarge

CPTC-CRABP2-2 Western Blot (Cell Lysate)

Result: Positive

Western blot using CPTC-CRABP2-2 as primary antibody against cell lysates and supernatants of cell lines OVCAR-3, OVCAR-4, OVAR-8 and SK-OV-3. Expected molecular weight is 15.6 KDa. Positive for OVCAR-3 cell lysates. Molecular weight standards are also included.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-CRABP2-2 as primary antibody against OVCAR-3 cell lysate. Molecular weight standards are also included. Click image to enlarge

CPTC-CRABP2-2 Western Blot (Cell Lysate)

Result: Positive

Automated western blot using CPTC-CRABP2-2 as primary antibody against OVCAR-3 cell lysate. Molecular weight standards are also included.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Single cell western blot using CPTC-CRABP2-2 as a primary antibody against cell lysates. Relative expression of total CRABP2 in OVCAR-3 cells (A). Percentage of cells that express CRABP2 (B). Average expression of CRABP2 protein per cell (C). All data is normalized to β-tubulin expression. Click image to enlarge

CPTC-CRABP2-2 Single Cell Western Blot (Cell Lysate)

Result: Positive

Single cell western blot using CPTC-CRABP2-2 as a primary antibody against cell lysates. Relative expression of total CRABP2 in OVCAR-3 cells (A). Percentage of cells that express CRABP2 (B). Average expression of CRABP2 protein per cell (C). All data is normalized to β-tubulin expression.


Characterization SOP Files

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Antigen: Cellular Retinoic Acid Binding Protein 2 Peptide 2
Background

NCI Identification Number:

00323

Antigen Name:

Cellular Retinoic Acid Binding Protein 2 Peptide 2

CPTC Name:

CPTC-CRABP2 Peptide 2

Aliases:

Cellular Retinoic Acid Binding Protein 2; CRABP-II; Cellular Retinoic Acid-Binding Protein II; CRABP2; RBP6

Function:

This gene encodes a member of the retinoic acid (RA, a form of vitamin A) binding protein family and lipocalin/cytosolic fatty-acid binding protein family. The protein is a cytosol-to-nuclear shuttling protein, which facilitates RA binding to its cognate receptor complex and transfer to the nucleus. It is involved in the retinoid signaling pathway, and is associated with increased circulating low-density lipoprotein cholesterol. Alternatively spliced transcript variants encoding the same protein have been found for this gene.CRABP2 (Cellular Retinoic Acid Binding Protein 2) is a Protein Coding gene. Diseases associated with CRABP2 include Embryonal Carcinoma and Hyperlipidemia, Familial Combined, 3. Among its related pathways are Signaling by GPCR and all-trans-Retinoic Acid Mediated Apoptosis. Gene Ontology (GO) annotations related to this gene include transporter activity and retinol binding. An important paralog of this gene is CRABP1.Transports retinoic acid to the nucleus. Regulates the access of retinoic acid to the nuclear retinoic acid receptors.

Chromosomal Localization:

1q23.1

Accession Number:

NP_001186652.1

UniProt Accession Number:

P29373

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

QEGDTFYIK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

990

Last Updated:

08/21/2020

Links

Characterization Data

SOPs

No SOPs available.

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