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Protein Tyrosine Phosphatase Non-Receptor Type 11 Peptide 1

Antibody: CPTC-PTPN11-1
Background

Catalog Number:

CPTC-PTPN11-1

Target Antigen:

Protein Tyrosine Phosphatase Non-Receptor Type 11 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

04/20/2023

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

Characterization Data [Compare Characterization Data]
  Affinity Measurement by Biolayer Interferometry
Click to enlarge image The affinity and binding kinetics of CPTC-PTPN11-1 antibody and BSA-conjugated phosphorylated peptide “IQNTGD-pY-YDLYGGEK" were measured using biolayer interferometry. BSA-conjugated peptide was covalently immobilized onto AR2G biosensors using standard amine coupling. CPTC-PTPN11-1 antibody, 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM, and 0.25 nM, was used as analyte. Buffer only and biosensors immobilized without BSA-conjugated peptide were used as references for background subtraction. All data was analyzed globally using a 1:2 bivalent model. Click image to enlarge

CPTC-PTPN11-1 Affinity and Kinetics (Bio-Layer Interferometry)

Result: High Binding

The affinity and binding kinetics of CPTC-PTPN11-1 antibody and BSA-conjugated phosphorylated peptide “IQNTGD-pY-YDLYGGEK" were measured using biolayer interferometry. BSA-conjugated peptide was covalently immobilized onto AR2G biosensors using standard amine coupling. CPTC-PTPN11-1 antibody, 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM, and 0.25 nM, was used as analyte. Buffer only and biosensors immobilized without BSA-conjugated peptide were used as references for background subtraction. All data was analyzed globally using a 1:2 bivalent model.


Characterization SOP Files

  Affinity Measurement by SPR
Click to enlarge image The affinity and binding kinetics of CPTC-PTPN11-1 antibody and BSA-conjugated phosphorylated-peptide, “IQNTGD-pY-YDLYGGEK” (A) and non-phosphorylated peptide (B), were measured using surface plasmon resonance. Peptide was amine coupled onto a Series S CM5 biosensor chip. Antibody at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1.0 nM and 0.25 nM, was used as analyte. Binding data were double-referenced and analyzed globally using a bivalent fitting model. Click image to enlarge

CPTC-PTPN11-1 Affinity and Kinetics (Surface Plasmon Resonance) 

Result: High Binding

The affinity and binding kinetics of CPTC-PTPN11-1 antibody and BSA-conjugated phosphorylated-peptide, “IQNTGD-pY-YDLYGGEK” (A) and non-phosphorylated peptide (B), were measured using surface plasmon resonance. Peptide was amine coupled onto a Series S CM5 biosensor chip. Antibody at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1.0 nM and 0.25 nM, was used as analyte. Binding data were double-referenced and analyzed globally using a bivalent fitting model.


Characterization SOP Files

  IHC Tissue

CPTC-PTPN11-1 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM using CPTC-PTPN11-1 as capture antibody against the phosphorylated synthetic peptide IQNTGD(pY)YDLYGGEK (phosphosite Y62) and the correspondent non-phosphorylated peptide IQNTGDYYDLYGGEK. Antibody CPTC-PTPN11-1 captures specifically the phosphorylated synthetic peptide, but not the correspondent non-phosphorylated peptide. Click image to enlarge

CPTC-PTPN11-1 iMRM (synthetic peptide)

Result: Positive

Immuno-MRM using CPTC-PTPN11-1 as capture antibody against the phosphorylated synthetic peptide IQNTGD(pY)YDLYGGEK (phosphosite Y62) and the correspondent non-phosphorylated peptide IQNTGDYYDLYGGEK. Antibody CPTC-PTPN11-1 captures specifically the phosphorylated synthetic peptide, but not the correspondent non-phosphorylated peptide.


Characterization SOP Files

  Immunofluorescence

CPTC-PTPN11-1 Immunofluorescence

Result: Negative

Immunofluorescence staining using CPTC-PTPN11-1 as primary antibody against MCF7 cells shows no localization of PTPN11 protein.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-PTPN11-1 as primary antibody against BSA conjugated phosphorylated peptide, "IQNTGD-pY-YDLYGGEK", coated on the plate and detected using HRP conjugated goat anti-rabbit secondary antibody and TMB. Click image to enlarge

CPTC-PTPN11-1 Indirect ELISA

Result: High Binding

Indirect ELISA using CPTC-PTPN11-1 as primary antibody against BSA conjugated phosphorylated peptide, "IQNTGD-pY-YDLYGGEK", coated on the plate and detected using HRP conjugated goat anti-rabbit secondary antibody and TMB.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-PTPN11-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-PTPN11-1 as primary antibody against human protein tyrosine phosphatase, non-receptor type 11 (PTPN11) recombinant protein. The expected molecular weight is 67.8 kDa. Click image to enlarge

CPTC-PTPN11-1 Western Blot (Recombinant Protein)

Result: Positive

Western blot using CPTC-PTPN11-1 as primary antibody against human protein tyrosine phosphatase, non-receptor type 11 (PTPN11) recombinant protein. The expected molecular weight is 67.8 kDa.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Automated western blot using CPTC-PTPN11-1 as primary antibody against recombinant PTPN11 protein. Protein molecular weight is about 68 KDa. The antibody presumably recognizes recombinant PTPN11. Click image to enlarge

CPTC- PTPN11-1 Automated Western Blot (Recombinant Protein)

Result: Presumed Positive (with additional bands)

Automated western blot using CPTC-PTPN11-1 as primary antibody against recombinant PTPN11 protein. Protein molecular weight is about 68 KDa. The antibody presumably recognizes recombinant PTPN11.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-PTPN11-1 as primary antibody against whole cell lysates CCRF-CEM (lane 2), HeLa (lane 3), Jurkat (lane 4), K-562 (lane 5), and MCF7 (lane 6). The expected molecular weights are 68.0 kDa, 68.4 kDa, and 52.8 kDa. Click image to enlarge

CPTC-PTPN11-1 Western Blot (Cell Lysates)

Result: Negative

Western blot using CPTC-PTPN11-1 as primary antibody against whole cell lysates CCRF-CEM (lane 2), HeLa (lane 3), Jurkat (lane 4), K-562 (lane 5), and MCF7 (lane 6). The expected molecular weights are 68.0 kDa, 68.4 kDa, and 52.8 kDa.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-PTPN11-1 as primary antibody against whole lysates of cell lines CCRF-CEM, HeLa, Jurkat, K-562 and MCF7. Protein molecular weight is about 68 KDa. The antibody recognizes PTPN11 in all the tested lysates. Loading controls were run with anti-GAPDH antibody. Click image to enlarge

CPTC-PTPN11-1 Automated Western Blot (Cell Lysates)

Result: Positive

Automated western blot using CPTC-PTPN11-1 as primary antibody against whole lysates of cell lines CCRF-CEM, HeLa, Jurkat, K-562 and MCF7. Protein molecular weight is about 68 KDa. The antibody recognizes PTPN11 in all the tested lysates. Loading controls were run with anti-GAPDH antibody.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-PTPN11-1 Single Cell Western Blot (Cell Lysate)

Result: Negative

Single cell western blot using CPTC-PTPN11-1 as a primary antibody against MCF7 cell lysate. PTPN11 protein expression was not detected.


Characterization SOP Files

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Antigen: Protein Tyrosine Phosphatase Non-Receptor Type 11 Peptide 1
Background

NCI Identification Number:

00525

Antigen Name:

Protein Tyrosine Phosphatase Non-Receptor Type 11 Peptide 1

CPTC Name:

CPTC-PTPN11 Peptide 1

Aliases:

Protein Tyrosine Phosphatase Non-Receptor Type 11; SH-PTP2; PTP2C; BPTP3; SHP-2; SHP2; SH2 Domain-Containing Protein Tyrosine Phosphatase 2; Tyrosine-Protein Phosphatase Non-Receptor Type 11; Protein-Tyrosine Phosphatase 1D; Protein-Tyrosine Phosphatase 2C; EC 3.1.3.48; SH-PTP3; PTP-1D; NS1; Protein Tyrosine Phosphatase, Non-Receptor Type 11; Noonan Syndrome 1; METCDS; SHPTP2; PTP-2C; JMML; Shp2; CFC

Function:

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia. [provided by RefSeq, Aug 2016]PTPN11 (Protein Tyrosine Phosphatase Non-Receptor Type 11) is a Protein Coding gene. Diseases associated with PTPN11 include Noonan Syndrome 1 and Juvenile Myelomonocytic Leukemia. Among its related pathways are SARS-CoV-2 Infection and Immune response IL-2 activation and signaling pathway. Gene Ontology (GO) annotations related to this gene include protein domain specific binding and protein tyrosine phosphatase activity. An important paralog of this gene is PTPN6. Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus (PubMed:10655584, PubMed:18559669, PubMed:18829466, PubMed:26742426, PubMed:28074573). Positively regulates MAPK signal transduction pathway (PubMed:28074573). Dephosphorylates GAB1, ARHGAP35 and EGFR (PubMed:28074573). Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulation of its RhoA binding activity (PubMed:18559669). Dephosphorylates CDC73 (PubMed:26742426). Dephosphorylates SOX9 on tyrosine residues, leading to inactivate SOX9 and promote ossification (By similarity). PTN11_HUMAN,Q06124Protein tyrosine phosphatases (PTPs) are a group of enzymes that catalyze the removal of phosphate groups from tyrosine residues by the hydrolysis of phosphoric acid monoesters. They directly oppose the actions of kinase and phosphorylase enzymes.

Chromosomal Localization:

12q24.13

Accession Number:

NP_002825.3

UniProt Accession Number:

Q06124

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

IQNTGD-pY-YDLYGGEK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1650

Last Updated:

02/13/2025

Links

Characterization Data

SOPs

No SOPs available.

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