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Kirsten Rat Sarcoma Viral Oncogene Homolog Peptide 1

Antibody: CPTC-KRAS4A-1
Background

Catalog Number:

CPTC-KRAS4A-1

Target Antigen:

Kirsten Rat Sarcoma Viral Oncogene Homolog Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

02/06/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

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Characterization Data [Compare Characterization Data]
  Affinity Measurement by Biolayer Interferometry
Click to enlarge image Affinity and binding kinetics of CPTC-KRAS4A-1 antibody and KRAS4A recombinant protein using biolayer interferometry. CPTC-KRAS4A-1 antibody was covalently immobilized on amine-reactive second-generation sensors. KRAS4A recombinant protein, 16 nM, 4.0 nM, 1 nM and 0.25 nM, was used as analyte. Click image to enlarge

CPTC-KRAS4A-1 Affinity and Kinetics

Result: High Binding

Affinity and binding kinetics of CPTC-KRAS4A-1 antibody and KRAS4A recombinant protein using biolayer interferometry. CPTC-KRAS4A-1 antibody was covalently immobilized on amine-reactive second-generation sensors. KRAS4A recombinant protein, 16 nM, 4.0 nM, 1 nM and 0.25 nM, was used as analyte.


Characterization SOP Files

  Affinity Measurement by SPR
Click to enlarge image Affinity and binding kinetics of CPTC-KRAS4A-1 and KRAS4A recombinant protein using surface plasmon resonance. CPTC-KRAS4A-1 antibody was captured onto a Series S Protein A biosensor chip. KRAS4A recombinant protein, 256 nM, 64 nM, 16 nM, 4 nM and 1.0 nM, was used as analyte. Click image to enlarge

CPTC-KRAS4A-1 Affinity and Kinetics

Result: High Binding

Affinity and binding kinetics of CPTC-KRAS4A-1 and KRAS4A recombinant protein using surface plasmon resonance. CPTC-KRAS4A-1 antibody was captured onto a Series S Protein A biosensor chip. KRAS4A recombinant protein, 256 nM, 64 nM, 16 nM, 4 nM and 1.0 nM, was used as analyte.


Characterization SOP Files

  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (304.9 KB)

CPTC-KRAS4A-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Micro-Array (TMA) core of colon cancer showing cytoplasmic and membranous staining using Antibody CPTC-KRAS4A-1. Titer: 1:2000 Click image to enlarge

CPTC-KRAS4A-1 IHC Tissue

Result: Positive

Tissue Micro-Array (TMA) core of colon cancer showing cytoplasmic and membranous staining using Antibody CPTC-KRAS4A-1. Titer: 1:2000


Characterization SOP Files

  Immunofluorescence
Click to enlarge image Immunofluorescence staining of Mouse Embryonic Fibroblasts (MEFs) stably transfected with KRAS4A-WT protein using CPTC-KRAS4A-1 Ab (green). KRAS4A protein expression shows localization to the nucleolar fibrillar center of the nucleolus. Click image to enlarge

CPTC-KRAS4A-1 Immunofluorescence

Result: Positive

Immunofluorescence staining of Mouse Embryonic Fibroblasts (MEFs) stably transfected with KRAS4A-WT protein using CPTC-KRAS4A-1 Ab (green). KRAS4A protein expression shows localization to the nucleolar fibrillar center of the nucleolus.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-KRAS4A-1 as primary antibody against KRAS4A recombinant protein. Click image to enlarge

CPTC-KRAS4A-1 Indirect ELISA

Result: High Binding

Indirect ELISA using CPTC-KRAS4A-1 as primary antibody against KRAS4A recombinant protein.


Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-KRAS4A-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-KRAS4A-1

Result: Positive

Protein Array in which CPTC-KRAS4A-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


Characterization SOP Files

  Western Blot - Over-expressed transient protein in cell lysate
Click to enlarge image Western Blot using CPTC-KRas4a-1 as primary antibody against cell lysates of Mouse Embryonic Fibroblasts (MEFs) stably transfected with KRas4a, KRas4b, HRAS and NRAS. The antibody showed specificity for KRas4a. Expected MW is ~21KDa. Click image to enlarge

CPTC-KRas4a-1 WB (Overexpressed Lysate)

Result: Positive

Western Blot using CPTC-KRas4a-1 as primary antibody against cell lysates of Mouse Embryonic Fibroblasts (MEFs) stably transfected with KRas4a, KRas4b, HRAS and NRAS. The antibody showed specificity for KRas4a. Expected MW is ~21KDa.


Characterization SOP Files

  Western Blot - Over-expressed transient protein in cell lysate
Click to enlarge image Automated Western Blot using CPTC-KRas4a-1 as primary antibody against cell lysates of Mouse Embryonic Fibroblasts (MEFs) stably transfected with KRas4a, KRas4b, HRAS and NRAS. The antibody showed specificity for KRAS4a. Expected MW is ~21KDa. Click image to enlarge

CPTC-KRas4a-1 Wes (Overexpressed Lysate)

Result: Positive

Automated Western Blot using CPTC-KRas4a-1 as primary antibody against cell lysates of Mouse Embryonic Fibroblasts (MEFs) stably transfected with KRas4a, KRas4b, HRAS and NRAS. The antibody showed specificity for KRAS4a. Expected MW is ~21KDa.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Single cell western blot for KRAS4A protein using CPTC-KRAS4A-1 as a primary antibody against whole cell lysates prepared from Mouse Embryonic Fibroblasts (MEFs) stably transfected with KRAS4A-WT protein. Relative expression of total KRAS4A (A). Percentage of cells that express KRAS4A (B). Average expression of KRAS4A protein per cell (C). All data is normalized to β-tubulin expression. Click image to enlarge

CPTC-KRAS4A-1 Single Cell Western Blot (Cell Lysate)

Result: Positive

Single cell western blot for KRAS4A protein using CPTC-KRAS4A-1 as a primary antibody against whole cell lysates prepared from Mouse Embryonic Fibroblasts (MEFs) stably transfected with KRAS4A-WT protein. Relative expression of total KRAS4A (A). Percentage of cells that express KRAS4A (B). Average expression of KRAS4A protein per cell (C). All data is normalized to β-tubulin expression.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-KRas4a-1 as primary antibody against cell lysates of HeLa, Jurkat, A549, MCF7 and H226. Presumed positive for MCF7. Expected MW is ~21KDa. Click image to enlarge

CPTC-KRas4a-1 WB (Lysates)

Result: Presumed Positive (with additional bands)

Western Blot using CPTC-KRas4a-1 as primary antibody against cell lysates of HeLa, Jurkat, A549, MCF7 and H226. Presumed positive for MCF7. Expected MW is ~21KDa.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Autometed Western Blot using CPTC-KRas4a-1as primary antibody against cell lysates of HeLa, Jurkat, A549, MCF7 and H226. Presumed positive for MCF7. Expected MW is ~21KDa. Click image to enlarge

CPTC-KRas4a-1 Wes (Lysates)

Result: Presumed Positive (with additional bands)

Autometed Western Blot using CPTC-KRas4a-1as primary antibody against cell lysates of HeLa, Jurkat, A549, MCF7 and H226. Presumed positive for MCF7. Expected MW is ~21KDa.


Characterization SOP Files

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Antigen: Kirsten Rat Sarcoma Viral Oncogene Homolog Peptide 1
Background

NCI Identification Number:

00124

Antigen Name:

Kirsten Rat Sarcoma Viral Oncogene Homolog Peptide 1

CPTC Name:

CPTC-KRAS4A Peptide 1

Aliases:

Kirsten Rat Sarcoma Viral Oncogene Homolog; KRAS2; RASK2; V-Ki-Ras2 Kirsten Rat Sarcoma 2 Viral Oncogene Homolog; C-Ki-Ras; K-Ras 2; C-K-RAS; KI-RAS; CFC2; NS; V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog; Cellular C-Ki-Ras2 Proto-Oncogene; Transforming Protein P21; PR310 C-K-Ras Oncogene; C-Kirsten-Ras Protein; K-Ras P21 Protein; Oncogene KRAS2; GTPase KRas; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KRAS1; NS3

Function:

This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.

KRAS (Kirsten Rat Sarcoma Viral Oncogene Homolog) is a Protein Coding gene. Diseases associated with KRAS include noonan syndrome 3 and cardiofaciocutaneous syndrome 2. Among its related pathways are PI3K-Akt signaling pathway and PI-3K cascade. GO annotations related to this gene include GTP binding and GDP binding. An important paralog of this gene is HRAS.

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation.

Chromosomal Localization:

12p12.1

Expression System:

E. coli

Accession Number:

NP_203524.1

UniProt Accession Number:

P01116

DNA Source:

N/A

Immunogen:

Recombinant Full Length Protein

Vector Name:

N/A

Extinction Coefficient:

Buffers:


Expressed Sequence:

QYRLKKISKEEKTPGCVKIKKC

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

2420

Last Updated:

09/23/2020

Links

Characterization Data

SOPs

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