Background
Catalog Number:
CPTC-KRT Group B-1
Target Antigen:
Keratin Group B Peptide 1
Isotype:
IgG
Species:
Rabbit Recombinant Cloned Antibody
Last Updated:
10/22/2021
Antigen Recognition(s):
Peptide, Recombinant Full-length, Endogenous
Purchase
- DSHB CPTC-KRT Group B-1
Characterization Data [Compare Characterization Data]
IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (324.0 KB)
CPTC-CPTC-KRT Group B-1 Evaluation by the Human Protein Atlas
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
IHC NCI60
CPTC-KRT Group B-1 IHC NCI60
Result: Positive
Immuno-histochemistry of CPTC-KRT Group B-1 for NCI60 Cell Line Array with titer 1:250
0=NEGATIVE
1=WEAK(red)
2=MODERATE(blue)
3=STRONG(green)
Characterization SOP Files
IHC Tissue
Download Tissue Micro-Array(TMA) core of pancreatic cancer showing cell membrane/cytoplasmic staining using Antibody CPTC-KRT Group B-1. Titer: 1:250 (3.2 MB)
CPTC-KRT Group B-1 IHC Tissue
Result: Positive
Tissue Micro-Array(TMA) core of pancreatic cancer showing cell membrane/cytoplasmic staining using Antibody CPTC-KRT Group B-1. Titer: 1:250
Characterization SOP Files
Immunofluorescence
CPTC-KRT Group B-1
Result: Positive
Immunofluorescence staining of human cell line HeLa with CPTC-KRT Group B-1 Ab shows localization to the cytoskeleton, cytosol, extracellular region or secreted, other.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-KRT Group B-1 Western Blot (Recombinant Protein)
Result: Positive
Western blot using CPTC-KRT Group B-1 as primary antibody against recombinant human Keratin 8 (KRT8) protein. Molecular weight standards are also included.
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-KRT Group B-1 Western Blot (Cell Lysate)
Result: Positive
Western blot using CPTC-KRT Group B-1 as primary antibody against LCL57, HeLa, and MCF10A cell lysates. Molecular weight standards are also included.
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-KRT Group B-1 Automated Western Blot (Cell Lysate)
Result: Positive
Automated western blot using CPTC-KRT Group B-1 as primary antibody against LCL57, HeLa, and MCF10A cell lysates. Molecular weight standard are also included.
Characterization SOP Files
Background
NCI Identification Number:
00457
Antigen Name:
Keratin Group B Peptide 1
CPTC Name:
CPTC-KRT Group B Peptide 1
Aliases:
Keratin 7; Keratin, Type II Cytoskeletal 7; Sarcolectin; SCL; K7; Keratin, 55K Type II Cytoskeletal; Type-II Keratin Kb7; Keratin 7, Type II; Cytokeratin 7; K2C7; CK-7; CK7; Keratin, Simple Epithelial Type I, K7; Type II Mesothelial Keratin K7; Keratin-7; KRT7; Keratin 8; CYK8; K8; Keratin, Type II Cytoskeletal 8; Type-II Keratin Kb8; Keratin 8, Type II; Cytokeratin-8; CARD2; CK-8; K2C8; CK8; KO; Keratin-8; KRT8
Function:
A peptide homologous to a group of Keratin Group B proteins (Keratin Group B: KRT7 KRT8 hCG_1988300) was used for antibody generation. KRT7: The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. KRT7 (Keratin 7) is a Protein Coding gene. Diseases associated with KRT7 include Pseudomyxoma Peritonei and Signet Ring Cell Adenocarcinoma. Among its related pathways are Cytoskeleton remodeling Neurofilaments and Developmental Biology. Gene Ontology (GO) annotations related to this gene include structural molecule activity. An important paralog of this gene is KRT5. Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).KRT8: This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. KRT8 (Keratin 8) is a Protein Coding gene. Diseases associated with KRT8 include Liver Cirrhosis and Cryptogenic Cirrhosis. Among its related pathways are Cytoskeleton remodeling Neurofilaments and Signaling mediated by p38-alpha and p38-beta. Gene Ontology (GO) annotations related to this gene include structural molecule activity and scaffold protein binding. An important paralog of this gene is KRT5. Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Chromosomal Localization:
KRT7: 12q13.13; KRT8: 12q13.13;
Accession Number:
N/A
UniProt Accession Number:
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
CELQSQISDTSVVLSMDNSR
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
2200
Last Updated:
10/02/2020
Links
Characterization Data
SOPs
No SOPs available.
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