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Inducible T Cell Costimulator Ligand Peptide 1

Antibody: CPTC-ICOSLG-1
Background

Catalog Number:

CPTC-ICOSLG-1

RRID:

AB_2889056

Target Antigen:

Inducible T Cell Costimulator Ligand Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

03/20/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

Thematic Panel(s):

Immuno-Oncology (IO)

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (336.9 KB)

CPTC-ICOSLG-1 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Micro-Array (TMA) core of lung cancer showing cytoplasmic and nuclear staining using Antibody CPTC-ICOSLG-1. Titer: 1:9000 Click image to enlarge

CTPC-ICOSLG-1 IHC Tissue

Result: Positive

Tissue Micro-Array (TMA) core of lung cancer showing cytoplasmic and nuclear staining using Antibody CPTC-ICOSLG-1. Titer: 1:9000


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-ICOSLG-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5937) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool. Click image to enlarge

CPTC-ICOSLG-1 iMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-ICOSLG-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5937)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool.


Characterization SOP Files

  Immunofluorescence

CPTC-ICOSLG-1 Immunofluorescence

Result: Negative

Immunofluorescence staining of human cell lines Jurkat, MCF7 and NCI H226 with CPTC-ICOSLG-1 Ab shows no localization of ICOSLG protein.


Characterization SOP Files

  Immunofluorescence

CPTC-ICOSLG-1 Immunofluorescence

Result: Negative

Immunofluorescence staining of HeLa cells using CPTC-ICOSLG-1 antibody. ICOSLG protein expression was not detected.


Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-ICOSLG-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Click image to enlarge

CTPC-ICOSLG-1 NCI60 Protein Array

Result: Positive

Protein Array in which CPTC-ICOSLG-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-ICOSLG-1 as primary antibody against human inducible T-cell co-stimulator ligand (ICOSLG) recombinant protein (lane 2). Expected molecular weight - 33.2 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-ICOSLG-1 Western Blot (recombinant protein)

Result: Positive

Western blot using CPTC-ICOSLG-1 as primary antibody against human inducible T-cell co-stimulator ligand (ICOSLG) recombinant protein (lane 2). Expected molecular weight - 33.2 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-ICOSLG-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) cell lysates. Expected molecular weight - 33.3 kDa. Molecular weight standards are also included (lane 1). Positive for buffy coat, negative for other cell lines. Click image to enlarge

CPTC-ICOSLG-1 Automated Western Blot (Cell Lysate)

Result: Positive

Automated western blot using CPTC-ICOSLG-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) cell lysates. Expected molecular weight - 33.3 kDa. Molecular weight standards are also included (lane 1). Positive for buffy coat, negative for other cell lines.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-ICOSLG-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5) and NCI H226 (lane 6) cell lysates. Expected molecular weight 33 kDa. Molecular weight standards (MW Stds.) are also included (lane 1). Inconclusive data. Click image to enlarge

CPTC-ICOSLG-1 Western Blot (Cell Lysate)

Result: Negative

Western blot using CPTC-ICOSLG-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5) and NCI H226 (lane 6) cell lysates. Expected molecular weight 33 kDa. Molecular weight standards (MW Stds.) are also included (lane 1). Inconclusive data.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-ICOSLG-1 Single Cell Western Blot (Cell Lysate)

Result: Negative

Single cell western blot using CPTC-ICOSLG-1 as a primary antibody against MCF7, NCI H226 and Jurkat cell lysates. ICOSLG protein expression was not detected.


Characterization SOP Files

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Antigen: Inducible T Cell Costimulator Ligand Peptide 1
Background

NCI Identification Number:

00372

Antigen Name:

Inducible T Cell Costimulator Ligand Peptide 1

CPTC Name:

CPTC-ICOSLG Peptide 1

Aliases:

Inducible T Cell Costimulator Ligand; B7-Related Protein 1; B7 Homolog 2; B7RP-1; B7-H2; B7RP1; B7H2; B7 Homologue 2; ICOS Ligand; KIAA0653; ICOS-L; CD275; ICOSL; GL50; B7h; Transmembrane Protein B7-H2 ICOS Ligand; Inducible T-Cell Costimulator Ligand; CD275 Antigen; ICOSLG; LICOS

Function:

ICOSLG (Inducible T Cell Costimulator Ligand) is a Protein Coding gene. Diseases associated with ICOSLG include Autoimmune Disease Of Exocrine System and Immune System Disease. Among its related pathways are ICos-ICosL Pathway in T-Helper Cell and Innate Immune System. Gene Ontology (GO) annotations related to this gene include signaling receptor binding. An important paralog of this gene is LOC102723996.Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function (By similarity).

Chromosomal Localization:

21q22.3

Accession Number:

NP_056074.1

UniProt Accession Number:

O75144

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

GLYDVVSVLR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1100

Last Updated:

09/02/2020

Links

Characterization Data

SOPs

No SOPs available.

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