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HRas Proto-Oncogene, GTPase

Antibody: CPTC-HRAS-1
Background

Catalog Number:

CPTC-HRAS-1

Target Antigen:

HRas Proto-Oncogene, GTPase

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

07/01/2025

Antigen Recognition(s):

Recombinant Full-length, Endogenous

External Links
Characterization Data [Compare Characterization Data]
  Affinity Measurement by Biolayer Interferometry
Click to enlarge image Affinity and binding kinetics of CPTC-HRAS-1 antibody and HRAS recombinant protein using biolayer interferometry. CPTC-HRAS-1 antibody was covalently immobilized on amine-reactive second-generation sensors. HRAS recombinant protein, 1024 nM, 256 nM, 64 nM, 16 nM, 4.0 nM and 1 nM, was used as analyte. Click image to enlarge

CPTC-HRAS-1 Affinity and Kinetics

Result: High Binding

Affinity and binding kinetics of CPTC-HRAS-1 antibody and HRAS recombinant protein using biolayer interferometry. CPTC-HRAS-1 antibody was covalently immobilized on amine-reactive second-generation sensors. HRAS recombinant protein, 1024 nM, 256 nM, 64 nM, 16 nM, 4.0 nM and 1 nM, was used as analyte.


Characterization SOP Files

  Affinity Measurement by SPR

CPTC-HRAS-1 Affinity and Kinetics

Result: No Binding

Affinity and binding kinetics of CPTC-HRAS-1 and HRAS recombinant protein using surface plasmon resonance. CPTC-HRAS-1 was amine coupled onto a Series S CM5 biosensor chip. HRAS recombinant protein, 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM and 1.0 nM, was used as analyte. Kinetic constant Ka is approaching the limits that can be measured by the instrument.


Characterization SOP Files

  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (467.3 KB)

CPTC-HRAS-1 evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  IHC Tissue

CPTC-HRAS-1 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  IP
Click to enlarge image Immuno-precipitation screening of antibody CPTC-HRAS-1 in cell lysates of Mouse Embryonic Fibroblasts (MEFs) stably transfected with KRas4a, KRas4b, HRAS and NRAS and in MCF7 cell lysate. IP eluates were tested in WB using CPTC-HRAS-1 as detection antibody (IP-WB), The antibody showed specificity for HRAS. Expected MW is ~21KDa. The target was also pulled down in the MCF7 lysate. Click image to enlarge

CPTC-HRAS-1 IP-WB

Result: Negative

Immuno-precipitation screening of antibody CPTC-HRAS-1 in cell lysates of Mouse Embryonic Fibroblasts (MEFs) stably transfected with KRas4a, KRas4b, HRAS and NRAS and in MCF7 cell lysate. IP eluates were tested in WB using CPTC-HRAS-1 as detection antibody (IP-WB), The antibody showed specificity for HRAS. Expected MW is ~21KDa. The target was also pulled down in the MCF7 lysate.


Characterization SOP Files

  Immunofluorescence
Click to enlarge image Immunofluorescence staining of Mouse Embryonic Fibroblasts (MEFs) stably transfected with HRAS-WT protein using CPTC-HRAS-1 antibody (green). HRAS protein expression shows localization to the nucleoplasm and cytosol. Click image to enlarge

CPTC-HRAS-1 Immunofluorescence

Result: Positive

Immunofluorescence staining of Mouse Embryonic Fibroblasts (MEFs) stably transfected with HRAS-WT protein using CPTC-HRAS-1 antibody (green). HRAS protein expression shows localization to the nucleoplasm and cytosol.


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (467.3 KB)

CPTC-HRAS-1 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-HRAS-1 as primary antibody against HRAS recombinant protein. Click image to enlarge

CPTC-HRAS-1 Indirect ELISA

Result: High Binding

Indirect ELISA using CPTC-HRAS-1 as primary antibody against HRAS recombinant protein.


Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-HRAS-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-HRAS-1

Result: Positive

Protein Array in which CPTC-HRAS-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


Characterization SOP Files

  Western Blot - Over-expressed transient protein in cell lysate
Click to enlarge image Western Blot using CPTC-HRAS-1 as primary antibody against cell lysates of Mouse Embryonic Fibroblasts (MEFs) stably transfected with KRas4a, KRas4b, HRAS and NRAS. The antibody showed specificity for HRAS. Expected MW is ~21KDa. Click image to enlarge

CPTC-HRAS-1 WB (Overexpressed Lysate)

Result: Positive

Western Blot using CPTC-HRAS-1 as primary antibody against cell lysates of Mouse Embryonic Fibroblasts (MEFs) stably transfected with KRas4a, KRas4b, HRAS and NRAS. The antibody showed specificity for HRAS. Expected MW is ~21KDa.


Characterization SOP Files

  Western Blot - Over-expressed transient protein in cell lysate
Click to enlarge image Automated Western Blot using CPTC-HRAS-1 as primary antibody against cell lysates of Mouse Embryonic Fibroblasts (MEFs) stably transfected with KRas4a, KRas4b, HRAS and NRAS. The antibody showed specificity for HRAS. Expected MW is ~21KDa. Click image to enlarge

CPTC-HRAS-1 Wes (Overexpressed Lysate)

Result: Positive

Automated Western Blot using CPTC-HRAS-1 as primary antibody against cell lysates of Mouse Embryonic Fibroblasts (MEFs) stably transfected with KRas4a, KRas4b, HRAS and NRAS. The antibody showed specificity for HRAS. Expected MW is ~21KDa.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Single cell western blot for HRAS protein using CPTC-HRAS-1 as a primary antibody against whole cell lysates prepared from Mouse Embryonic Fibroblasts (MEFs) stably transfected with HRAS-WT protein. Relative expression of total HRAS (A). Percentage of cells that express HRAS (B). Average expression of HRAS protein per cell (C). Molecular weight detected is higher than expected. All data is normalized to β-tubulin expression. Click image to enlarge

CPTC-HRAS-1 Single Cell Western Blot (Cell Lysate)

Result: Positive

Single cell western blot for HRAS protein using CPTC-HRAS-1 as a primary antibody against whole cell lysates prepared from Mouse Embryonic Fibroblasts (MEFs) stably transfected with HRAS-WT protein. Relative expression of total HRAS (A). Percentage of cells that express HRAS (B). Average expression of HRAS protein per cell (C). Molecular weight detected is higher than expected. All data is normalized to β-tubulin expression.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-HRAS-1 as primary antibody against cell lysates of HeLa, Jurkat, A549, MCF7 and H226. Presumed positive for MCF7. Expected MW is ~21KDa. Click image to enlarge

CPTC-HRAS-1 WB (Lysates)

Result: Presumed Positive (with additional bands)

Western Blot using CPTC-HRAS-1 as primary antibody against cell lysates of HeLa, Jurkat, A549, MCF7 and H226. Presumed positive for MCF7. Expected MW is ~21KDa.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated Western Blot using CPTC-HRAS-1 as primary antibody against cell lysates of HeLa, Jurkat, A549, MCF7 and H226. Positive for MCF7. Expected MW is ~21KDa. Click image to enlarge

CPTC-HRAS-1 Wes (Lysates)

Result: Positive

Automated Western Blot using CPTC-HRAS-1 as primary antibody against cell lysates of HeLa, Jurkat, A549, MCF7 and H226. Positive for MCF7. Expected MW is ~21KDa.


Characterization SOP Files

[top]

Antibody: CPTC-HRAS-2
Background

Catalog Number:

CPTC-HRAS-2

Target Antigen:

HRas Proto-Oncogene, GTPase

Isotype:

IgG2a

Species:

Mouse Monoclonal Antibody

Last Updated:

07/01/2025

Antigen Recognition(s):

Recombinant Full-length, Endogenous

External Links
Characterization Data [Compare Characterization Data]
  Affinity Measurement by Biolayer Interferometry
Click to enlarge image Affinity and binding kinetics of CPTC-HRAS-2 antibody and HRAS recombinant protein using biolayer interferometry. CPTC-HRAS-2 antibody was covalently immobilized on amine-reactive second-generation sensors. HRAS recombinant protein, 64 nM, 16 nM, 4.0 nM, 1 nM and 0.25 nM, was used as analyte. Click image to enlarge

CPTC-HRAS-2 Affinity and Kinetics

Result: High Binding

Affinity and binding kinetics of CPTC-HRAS-2 antibody and HRAS recombinant protein using biolayer interferometry. CPTC-HRAS-2 antibody was covalently immobilized on amine-reactive second-generation sensors. HRAS recombinant protein, 64 nM, 16 nM, 4.0 nM, 1 nM and 0.25 nM, was used as analyte.


Characterization SOP Files

  Affinity Measurement by SPR
Click to enlarge image Affinity and binding kinetics of CPTC-HRAS-2 and HRAS recombinant protein using surface plasmon resonance. CPTC-HRAS-2 was amine coupled onto a Series S CM5 biosensor chip. HRAS recombinant protein, 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM and 0.25 nM, was used as analyte. Click image to enlarge

CPTC-HRAS-2 Affinity and Kinetics

Result: High Binding

Affinity and binding kinetics of CPTC-HRAS-2 and HRAS recombinant protein using surface plasmon resonance. CPTC-HRAS-2 was amine coupled onto a Series S CM5 biosensor chip. HRAS recombinant protein, 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM and 0.25 nM, was used as analyte.


Characterization SOP Files

  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (518.4 KB)

CPTC-HRAS-2 evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Micro-Array (TMA) core of breast cancer showing cytoplasmic and membranous staining using Antibody CPTC-HRAS-2. Titer: 1:500 Click image to enlarge

CPTC-HRAS-2 IHC Tissue

Result: Positive

Tissue Micro-Array (TMA) core of breast cancer showing cytoplasmic and membranous staining using Antibody CPTC-HRAS-2. Titer: 1:500


Characterization SOP Files

  IP
Click to enlarge image Immuno-precipitation screening of antibody CPTC-HRAS-2 in cell lysates of Mouse Embryonic Fibroblasts (MEFs) stably transfected with KRas4a, KRas4b, HRAS and NRAS and in MCF7 cell lysate. IP eluates were tested in WB using CPTC-HRAS-1 as detection antibody (IP-WB), The antibody showed specificity for HRAS. Expected MW is ~21KDa. The target was also pulled down in the MCF7 lysate. Click image to enlarge

CPTC-HRAS-2 IP-WB

Result: Positive

Immuno-precipitation screening of antibody CPTC-HRAS-2 in cell lysates of Mouse Embryonic Fibroblasts (MEFs) stably transfected with KRas4a, KRas4b, HRAS and NRAS and in MCF7 cell lysate. IP eluates were tested in WB using CPTC-HRAS-1 as detection antibody (IP-WB), The antibody showed specificity for HRAS. Expected MW is ~21KDa. The target was also pulled down in the MCF7 lysate.


Characterization SOP Files

  Immunofluorescence

CPTC-HRAS-2 Immunofluorescence

Result: Negative

Immunofluorescence staining of Mouse Embryonic Fibroblasts (MEFs) stably transfected with HRAS-WT protein using CPTC-HRAS-2 antibody. HRAS protein expression was not detected.


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (518.4 KB)

CPTC-HRAS-2 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-HRAS-2 as primary antibody against HRAS recombinant protein. Click image to enlarge

CPTC-HRAS-2 Indirect ELISA

Result: High Binding

Indirect ELISA using CPTC-HRAS-2 as primary antibody against HRAS recombinant protein.


Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-HRAS-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-HRAS-2

Result: Positive

Protein Array in which CPTC-HRAS-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


Characterization SOP Files

  Western Blot - Over-expressed transient protein in cell lysate
Click to enlarge image Western Blot using CPTC-HRAS-2 as primary antibody against cell lysates of Mouse Embryonic Fibroblasts (MEFs) stably transfected with KRas4a, KRas4b, HRAS and NRAS. The antibody showed specificity for HRAS. Expected MW is ~21KDa. Click image to enlarge

CPTC-HRAS-2 WB (Overexpressed Lysate)

Result: Positive

Western Blot using CPTC-HRAS-2 as primary antibody against cell lysates of Mouse Embryonic Fibroblasts (MEFs) stably transfected with KRas4a, KRas4b, HRAS and NRAS. The antibody showed specificity for HRAS. Expected MW is ~21KDa.


Characterization SOP Files

  Western Blot - Over-expressed transient protein in cell lysate
Click to enlarge image Automated Western Blot using CPTC-HRAS-2 as primary antibody against cell lysates of Mouse Embryonic Fibroblasts (MEFs) stably transfected with KRas4a, KRas4b, HRAS and NRAS. The antibody showed specificity for HRAS. Expected MW is ~21KDa. Click image to enlarge

CPTC-HRAS-2 Wes (Overexpressed Lysate)

Result: Positive

Automated Western Blot using CPTC-HRAS-2 as primary antibody against cell lysates of Mouse Embryonic Fibroblasts (MEFs) stably transfected with KRas4a, KRas4b, HRAS and NRAS. The antibody showed specificity for HRAS. Expected MW is ~21KDa.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Single cell western blot for HRAS protein using CPTC-HRAS-2 as a primary antibody against whole cell lysates prepared from Mouse Embryonic Fibroblasts (MEFs) stably transfected with HRAS-WT protein. Relative expression of total HRAS (A). Percentage of cells that express HRAS (B). Average expression of HRAS protein per cell (C). Molecular weight detected is higher than expected. All data is normalized to β-tubulin expression. Click image to enlarge

CPTC-HRAS-2 Single Cell Western Blot (Cell Lysate)

Result: Positive

Single cell western blot for HRAS protein using CPTC-HRAS-2 as a primary antibody against whole cell lysates prepared from Mouse Embryonic Fibroblasts (MEFs) stably transfected with HRAS-WT protein. Relative expression of total HRAS (A). Percentage of cells that express HRAS (B). Average expression of HRAS protein per cell (C). Molecular weight detected is higher than expected. All data is normalized to β-tubulin expression.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-HRAS-2 as primary antibody against cell lysates of HeLa, Jurkat, A549, MCF7 and H226. Presumed positive for MCF7. Expected MW is ~21KDa. Click image to enlarge

CPTC-HRAS-2 WB (Lysates)

Result: Presumed Positive (with additional bands)

Western Blot using CPTC-HRAS-2 as primary antibody against cell lysates of HeLa, Jurkat, A549, MCF7 and H226. Presumed positive for MCF7. Expected MW is ~21KDa.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Autometed Western Blot using CPTC-HRAS-2 as primary antibody against cell lysates of HeLa, Jurkat, A549, MCF7 and H226. Presumed positive for MCF7. Expected MW is ~21KDa. Click image to enlarge

CPTC-HRAS-2 Wes (Lysates)

Result: Presumed Positive (with additional bands)

Autometed Western Blot using CPTC-HRAS-2 as primary antibody against cell lysates of HeLa, Jurkat, A549, MCF7 and H226. Presumed positive for MCF7. Expected MW is ~21KDa.


Characterization SOP Files

[top]

Antibody: CPTC-HRAS-3
Background

Catalog Number:

CPTC-HRAS-3

Target Antigen:

HRas Proto-Oncogene, GTPase

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

07/01/2025

Antigen Recognition(s):

Recombinant Full-length, Endogenous

External Links
Characterization Data [Compare Characterization Data]
  Affinity Measurement by Biolayer Interferometry
Click to enlarge image Affinity and binding kinetics of CPTC-HRAS-3 antibody and HRAS recombinant protein using biolayer interferometry. CPTC-HRAS-3 antibody was covalently immobilized on amine-reactive second-generation sensors. HRAS recombinant protein, 256 nM, 64 nM, 16 nM, 4.0 nM, 1 nM and 0.25 nM, was used as analyte. Click image to enlarge

CPTC-HRAS-3 Affinity and Kinetics

Result: High Binding

Affinity and binding kinetics of CPTC-HRAS-3 antibody and HRAS recombinant protein using biolayer interferometry. CPTC-HRAS-3 antibody was covalently immobilized on amine-reactive second-generation sensors. HRAS recombinant protein, 256 nM, 64 nM, 16 nM, 4.0 nM, 1 nM and 0.25 nM, was used as analyte.


Characterization SOP Files

  Affinity Measurement by SPR

CPTC-HRAS-3 Affinity and Kinetics

Result: No Binding

Affinity and binding kinetics of CPTC-HRAS-3 and HRAS recombinant protein using surface plasmon resonance. CPTC-HRAS-3 was amine coupled onto a Series S CM5 biosensor chip. KRAS4A recombinant protein, 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM and 1.0 nM, was used as analyte. Kinetic constant Ka is approaching the limits that can be measured by the instrument.


Characterization SOP Files

  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (495.5 KB)

CPTC-HRAS-3 evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  IHC Tissue

CPTC-HRAS-3 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  IP
Click to enlarge image Immuno-precipitation screening of antibody CPTC-HRAS-3 in cell lysates of Mouse Embryonic Fibroblasts (MEFs) stably transfected with KRas4a, KRas4b, HRAS and NRAS and in MCF7 cell lysate. IP eluates were tested in WB using CPTC-HRAS-1 as detection antibody (IP-WB), The antibody showed specificity for HRAS. Expected MW is ~21KDa. The target was also pulled down in the MCF7 lysate. Click image to enlarge

CPTC-HRAS-3 IP-WB

Result: Negative

Immuno-precipitation screening of antibody CPTC-HRAS-3 in cell lysates of Mouse Embryonic Fibroblasts (MEFs) stably transfected with KRas4a, KRas4b, HRAS and NRAS and in MCF7 cell lysate. IP eluates were tested in WB using CPTC-HRAS-1 as detection antibody (IP-WB), The antibody showed specificity for HRAS. Expected MW is ~21KDa. The target was also pulled down in the MCF7 lysate.


Characterization SOP Files

  Immunofluorescence

CPTC-HRAS-3 Immunofluorescence

Result: Negative

Immunofluorescence staining of Mouse Embryonic Fibroblasts (MEFs) stably transfected with HRAS-WT protein using CPTC-HRAS-3 antibody. HRAS protein expression was not detected.


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (495.5 KB)

CPTC-HRAS-3 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-HRAS-3 as primary antibody against HRAS recombinant protein. Click image to enlarge

CPTC-HRAS-3 Indirect ELISA

Result: High Binding

Indirect ELISA using CPTC-HRAS-3 as primary antibody against HRAS recombinant protein.


Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-HRAS-3 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-HRAS-3

Result: Positive

Protein Array in which CPTC-HRAS-3 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


Characterization SOP Files

  Western Blot - Over-expressed transient protein in cell lysate
Click to enlarge image Western Blot using CPTC-HRAS-3 as primary antibody against cell lysates of Mouse Embryonic Fibroblasts (MEFs) stably transfected with KRas4a, KRas4b, HRAS and NRAS. The antibody showed specificity for HRAS. Expected MW is ~21KDa. Click image to enlarge

CPTC-HRAS-3 WB (Overexpressed Lysate)

Result: Positive

Western Blot using CPTC-HRAS-3 as primary antibody against cell lysates of Mouse Embryonic Fibroblasts (MEFs) stably transfected with KRas4a, KRas4b, HRAS and NRAS. The antibody showed specificity for HRAS. Expected MW is ~21KDa.


Characterization SOP Files

  Western Blot - Over-expressed transient protein in cell lysate
Click to enlarge image Automated Western Blot using CPTC-HRAS-3 as primary antibody against cell lysates of Mouse Embryonic Fibroblasts (MEFs) stably transfected with KRas4a, KRas4b, HRAS and NRAS. The antibody showed specificity for HRAS. Expected MW is ~21KDa. Click image to enlarge

CPTC-HRAS-3 Wes (Overexpressed Lysate)

Result: Positive

Automated Western Blot using CPTC-HRAS-3 as primary antibody against cell lysates of Mouse Embryonic Fibroblasts (MEFs) stably transfected with KRas4a, KRas4b, HRAS and NRAS. The antibody showed specificity for HRAS. Expected MW is ~21KDa.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Single cell western blot for HRAS protein using CPTC-HRAS-3 as a primary antibody against whole cell lysates prepared from Mouse Embryonic Fibroblasts (MEFs) stably transfected with HRAS-WT protein. Relative expression of total HRAS (A). Percentage of cells that express HRAS (B). Average expression of HRAS protein per cell (C). Molecular weight detected is higher than expected. All data is normalized to β-tubulin expression. Click image to enlarge

CPTC-HRAS-3 Single Cell Western Blot (Cell Lysate)

Result: Positive

Single cell western blot for HRAS protein using CPTC-HRAS-3 as a primary antibody against whole cell lysates prepared from Mouse Embryonic Fibroblasts (MEFs) stably transfected with HRAS-WT protein. Relative expression of total HRAS (A). Percentage of cells that express HRAS (B). Average expression of HRAS protein per cell (C). Molecular weight detected is higher than expected. All data is normalized to β-tubulin expression.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-HRAS-3 as primary antibody against cell lysates of HeLa, Jurkat, A549, MCF7 and H226. Presumed positve for MCF7. Expected MW is ~21KDa. Click image to enlarge

CPTC-HRAS-3 WB (Lysates)

Result: Presumed Positive (with additional bands)

Western Blot using CPTC-HRAS-3 as primary antibody against cell lysates of HeLa, Jurkat, A549, MCF7 and H226. Presumed positve for MCF7. Expected MW is ~21KDa.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Autometed Western Blot using CPTC-HRAS-3 as primary antibody against cell lysates of HeLa, Jurkat, A549, MCF7 and H226. Positive for MCF7. Expected MW is ~21KDa. Click image to enlarge

CPTC-HRAS-3 Wes (Lysates)

Result: Positive

Autometed Western Blot using CPTC-HRAS-3 as primary antibody against cell lysates of HeLa, Jurkat, A549, MCF7 and H226. Positive for MCF7. Expected MW is ~21KDa.


Characterization SOP Files

[top]

Antibody: CPTC-HRAS-4
Background

Catalog Number:

CPTC-HRAS-4

Target Antigen:

HRas Proto-Oncogene, GTPase

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

05/15/2025

Antigen Recognition(s):

Recombinant Full-length

Characterization Data [Compare Characterization Data]
  CyTOF
Click to enlarge image Imaging mass cytometry on ovarian cancer tissue core using CPTC-HRAS-4 metal-labeled antibody. Data shows overlay of target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (testis, colon, endometrium, appendix, bone marrow, breast, kidney, and lung) and cancer tissues (breast, colon, ovarian, lung, and prostate). Click image to enlarge

CPTC-HRAS-4 Immuno-Mass Cytometry

Result: Positive

Imaging mass cytometry on ovarian cancer tissue core using CPTC-HRAS-4 metal-labeled antibody. Data shows overlay of target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (testis, colon, endometrium, appendix, bone marrow, breast, kidney, and lung) and cancer tissues (breast, colon, ovarian, lung, and prostate).


Characterization SOP Files

  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (533.0 KB)

CPTC-HRAS-4 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (533.0 KB)

CPTC-HRAS-4 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

[top]

Antigen: HRas Proto-Oncogene, GTPase
Background

NCI Identification Number:

00490

Antigen Name:

HRas Proto-Oncogene, GTPase

CPTC Name:

CPTC-HRAS

Aliases:

HRas Proto-Oncogene, GTPase; V-Ha-Ras Harvey Rat Sarcoma Viral Oncogene Homolog; Harvey Rat Sarcoma Viral Oncogene Homolog; Transforming Protein P21; GTPase HRas; P21ras; HRAS1; Ras Family Small GTP Binding Protein H-Ras; Harvey Rat Sarcoma Viral Oncoprotein; Transformation Gene: Oncogene HAMSV; GTP- And GDP-Binding Peptide B; Ha-Ras1 Proto-Oncoprotein; C-Has/Bas P21 Protein; P19 H-RasIDX Protein; EC 3.6.5.2; C-BAS/HAS; C-HA-RAS1; H-RASIDX; C-H-RAS; H-Ras-1; C-H-Ras; Ha-Ras; HAMSV; RASH1; CTLO; HRAS

Function:

This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, cognitive disability, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene.HRAS (HRas Proto-Oncogene, GTPase) is a Protein Coding gene. Diseases associated with HRAS include Costello Syndrome and Nevus, Epidermal. Among its related pathways are Sertoli-Sertoli Cell Junction Dynamics and ERK Signaling. Gene Ontology (GO) annotations related to this gene include GTP binding and protein C-terminus binding. An important paralog of this gene is KRAS.Involved in the activation of Ras protein signal transduction (PubMed:22821884). Ras proteins bind GDP/GTP and possess intrinsic GTPase activity

Chromosomal Localization:

11p15.5

Expression System:

Baculovirus

Accession Number:

NP_005334.1

UniProt Accession Number:

P01112

DNA Source:

N/A

Immunogen:

Recombinant Full Length Protein

Vector Name:

xx

Extinction Coefficient:

Buffers:

Expressed Sequence:

MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGET
CLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQI
KRVKDSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQ
GVEDAFYTLVREIRQHKLRKLNPPDESGPGCMSCKCVLS

Native Sequence:

Calculated Isoelectric Point:

5.16

Molecular Weight:

21355

Last Updated:

11/17/2021

Links

Characterization Data

SOPs

No SOPs available.

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