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Zinc Finger E-Box Binding Homeobox 1 Peptide 2

Antibody: CPTC-ZEB1-3
Background

Catalog Number:

CPTC-ZEB1-3

RRID:

AB_2827835

Target Antigen:

Zinc Finger E-Box Binding Homeobox 1 Peptide 2

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

02/09/2024

Antigen Recognition(s):

Peptide, Endogenous

Thematic Panel(s):

Epithelial Mesenchymal Transition (EMT)

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (398.9 KB)

CPTC-ZEB1-3 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IP
Download Immuno-precipitation data obtained incubating CPTC-ZEB1-3 and the recombinant domain of ZEB1 (aa503-1003, expected, MW ~46KDa) (149.9 KB)

CPTC-ZEB1-3 IPMS

Result: Negative

Immuno-precipitation data obtained incubating CPTC-ZEB1-3 and the recombinant domain of ZEB1 (aa503-1003, expected, MW ~46KDa)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-ZEB1-3 as primary antibody against ZEB1 domain comprising amino acids 503-1003. Click image to enlarge

CPTC-ZEB1-3 Indirect ELISA

Result: High Binding

Indirect ELISA using CPTC-ZEB1-3 as primary antibody against ZEB1 domain comprising amino acids 503-1003.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide

CPTC-ZEB1-3 WB

Result: Negative

Western Blot using CPTC-ZEB1-3 as primary antibody against recombinant ZEB1 protein (lane 2) with expected MW of 124 KDa.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-ZEB1-3 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 124 KDa. Positive for A549A and MCF7, incoclusive/negative for other cell lysates. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-ZEB1-3 Endogenous Western Blot

Result: Positive

Western Blot using CPTC-ZEB1-3 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 124 KDa. Positive for A549A and MCF7, incoclusive/negative for other cell lysates. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated Western Blot using CPTC-ZEB1-3 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 124 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-ZEB1-3 Endogenous Automated Western Blot

Result: Negative

Automated Western Blot using CPTC-ZEB1-3 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 124 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1).


Characterization SOP Files

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Antigen: Zinc Finger E-Box Binding Homeobox 1 Peptide 2
Background

NCI Identification Number:

00073

Antigen Name:

Zinc Finger E-Box Binding Homeobox 1 Peptide 2

CPTC Name:

CPTC-ZEB1 Peptide 2

Aliases:

TCF8; PPCD3; Posterior Polymorphous Corneal Dystrophy 3; Transcription Factor 8 (Represses Interleukin 2 Expression); Negative Regulator Of IL2; AREB6; FECD6; NIL2A; BZP; DELTAEF1; ZFHEP; ZFHX1A; Delta-Crystallin Enhancer Binding Factor 1; Zinc Finger E-Box-Binding Homeobox 1; Zinc Finger Homeodomain Enhancer-Binding Protein; TCF-8; NIL-2-A Zinc Finger Protein; Transcription Factor 8

Function:

Chromosomal Localization:

10p11.22

Accession Number:

NP_001167564.1

UniProt Accession Number:

P37275

DNA Source:

The NCI acknowledges the generous advice and gene clones provided by Robert A. Weinberg at the Ludwig Center for Cancer Research of the Massachusetts Institute of Technology. These reagents have been provided by the Division of Cancer Treatment and Diagnosis (DCTD) of the National Cancer Institute (NCI) using funding of the American Recovery and Reinvestment Act of 2009

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

cSRNTQGYLYTAEGAQEEPQVEPLD

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

2750

Last Updated:

09/16/2019

Links

Characterization Data

SOPs

No SOPs available.

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