Vascular Cell Adhesion Molecule 1 Peptide 1


Background

Catalog Number:

CPTC-VCAM1-1

RRID:

AB_2889970

Target Antigen:

Vascular Cell Adhesion Molecule 1 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

08/26/2022

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

Thematic Panel(s):

Immuno-Oncology (IO)

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (297.0 KB)

CPTC-VCAM1-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


  IHC Tissue
Click to enlarge image Tissue Micro-Array (TMA) core of ovarian cancer  showing cytoplasmic and membranous staining using Antibody CPTC-VCAM1-1. Titer: 1:1000 Click image to enlarge

CTPC-VCAM1-1 IHC Tissue

Result: Positive

Tissue Micro-Array (TMA) core of ovarian cancer showing cytoplasmic and membranous staining using Antibody CPTC-VCAM1-1. Titer: 1:1000


  Immunofluorescence

CPTC-VCAM1-1 Immunofluorescence

Result: Negative

Immunofluorescence staining of human cell lines Jurkat, MCF7 and NCI H226 with CPTC-VCAM1-1 Ab shows no localization of VCAM1 protein.


  NCI 60 Protein Array

CTPC-VCAM1-1 NCI60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-VCAM1-1 as primary antibody against human vascular cell adhesion molecule 1 (VCAM1), transcript variant 1 recombinant protein (lane 2). Expected molecular weight - 78.7 kDa.  Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-VCAM1-1 Western Blot (recombinant protein)

Result: Positive

Western blot using CPTC-VCAM1-1 as primary antibody against human vascular cell adhesion molecule 1 (VCAM1), transcript variant 1 recombinant protein (lane 2). Expected molecular weight - 78.7 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-VCAM1-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) cell lysates.  Expected molecular weight - 81 kDa.  Molecular weight standards are also included (lane 1). Data is positive for HeLa, A549, and MCF7. Data is negative/inconclusive for remaining cell lines. Click image to enlarge

CPTC-VCAM1-1 Automated Western Blot (Cell Lysate)

Result: Positive

Automated western blot using CPTC-VCAM1-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) cell lysates. Expected molecular weight - 81 kDa. Molecular weight standards are also included (lane 1). Data is positive for HeLa, A549, and MCF7. Data is negative/inconclusive for remaining cell lines.


  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-VCAM1-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5) and NCI H226 (lane 6) cell lysates.  Expected molecular weight 81 kDa.  Molecular weight standards (MW Stds.) are also included (lane 1).  Positive for cell line NCI H226. Inconclusive/negative for the other cell lines. Click image to enlarge

CPTC-VCAM1-1 Western Blot (Cell Lysate)

Result: Positive

Western blot using CPTC-VCAM1-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5) and NCI H226 (lane 6) cell lysates. Expected molecular weight 81 kDa. Molecular weight standards (MW Stds.) are also included (lane 1). Positive for cell line NCI H226. Inconclusive/negative for the other cell lines.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-VCAM1-1 Single Cell Western Blot (Cell Lysate)

Result: Negative

Single cell western blot using CPTC-VCAM1-1 as a primary antibody against MCF7, NCI H226 and Jurkat cell lysates. VCAM1 protein expression was not detected.


Background

NCI Identification Number:

00401

Antigen Name:

Vascular Cell Adhesion Molecule 1 Peptide 1

CPTC Name:

CPTC-VCAM1 Peptide 1

Aliases:

Vascular Cell Adhesion Molecule 1; Vascular Cell Adhesion Protein 1; CD106 Antigen; INCAM-100; CD106; V-CAM 1; VCAM-1; VCAM1

Function:

This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene.VCAM1 (Vascular Cell Adhesion Molecule 1) is a Protein Coding gene. Diseases associated with VCAM1 include Viral Encephalitis and Chronic Venous Insufficiency. Among its related pathways are Degradation of the extracellular matrix and Cellular roles of Anthrax toxin. Gene Ontology (GO) annotations related to this gene include integrin binding and primary amine oxidase activity. An important paralog of this gene is MXRA5.Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.

Chromosomal Localization:

1p21.2

Accession Number:

NP_001069.1

UniProt Accession Number:

P19320

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

TQIDSPLSGK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1100

Last Updated:

09/02/2020

Links

Characterization Data

SOPs

No SOPs available.

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