Background
Catalog Number:
CPTC-TAPBP-1
RRID:
AB_2814788
Target Antigen:
TAP Binding Protein Peptide 1
Isotype:
IgG
Species:
Mouse Monoclonal Antibody
Last Updated:
02/15/2024
Antigen Recognition(s):
Peptide
Purchase
- DSHB CPTC-TAPBP-1
Characterization Data [Compare Characterization Data]
IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (474.0 KB)
CPTC-TAPBP-1 evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (474.0 KB)
CPTC-TAPBP-1 evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
Background
Catalog Number:
CPTC-TAPBP-2
RRID:
AB_2814789
Target Antigen:
TAP Binding Protein Peptide 1
Isotype:
IgG
Species:
Mouse Monoclonal Antibody
Last Updated:
02/15/2024
Antigen Recognition(s):
Peptide, Endogenous
Purchase
- DSHB CPTC-TAPBP-2
External Links
- Human Protein Atlas CPTC-TAPBP-2
Characterization Data [Compare Characterization Data]
IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (424.5 KB)
CPTC-TAPBP-2 evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
IHC Tissue
CPTC-TAPBP-2 IHC Tissue
Result: Presumed Positive
Tissue Micro-Array (TMA) core of breast cancer showing cytoplasmic staining using Antibody CPTC-TAPBP-2 . Titer: 1:500.
The pattern of staining is correct but lacks sufficient literature data to confirm cell type specificity.
Characterization SOP Files
Immunofluorescence - HPA
CPTC-TAPBP-2 evaluation by the Human Protein Atlas
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org).
The subcellular location is partly supported by literature or no literature is available. Immunofluorescent staining of human cell line THP-1 shows localization to nucleoli & plasma membrane.
Human assay: THP-1 fixed with PFA, dilution: 1:200
Human assay: U2OS fixed with PFA, dilution: 1:200
Characterization SOP Files
NCI 60 Protein Array
CPTC-TAPBP-2 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-TAPBP-2 Western Blot Recombinant Protein
Result: Negative
Western blot using CPTC-TAPBP-2 as primary antibody against human TAP binding protein (tapasin) (TAPBP), transcript variant 1, recombinant protein (lane 2). Expected molecular weight - 45.6 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-TAPBP-2 Automated Western Blot Cell Lysate
Result: Negative
Automated western blot using CPTC-TAPBP-2 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 47.6 kDa, 43.9 kDa, 53.9 kDa, and 38.4 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-TAPBP-2 Western Blot Cell Lysate
Result: Negative
Western blot using CPTC-TAPBP-2 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 47.6 kDa, 43.9 kDa, 53.9 kDa, and 38.4 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Background
NCI Identification Number:
00479
Antigen Name:
TAP Binding Protein Peptide 1
CPTC Name:
CPTC-TAPBP Peptide 1
Aliases:
TAP Binding Protein; Tapasin; TAPA; TAP Binding Protein (Tapasin); TAP-Associated Protein; NGS-17; NGS17; TPSN; TPN; TAP-Binding Protein; TAPBP
Function:
This gene encodes a transmembrane glycoprotein which mediates interaction between newly assembled major histocompatibility complex (MHC) class I molecules and the transporter associated with antigen processing (TAP), which is required for the transport of antigenic peptides across the endoplasmic reticulum membrane. This interaction is essential for optimal peptide loading on the MHC class I molecule. Up to four complexes of MHC class I and this protein may be bound to a single TAP molecule. This protein contains a C-terminal double-lysine motif (KKKAE) known to maintain membrane proteins in the endoplasmic reticulum. This gene lies within the major histocompatibility complex on chromosome 6. Alternative splicing results in three transcript variants encoding different isoforms.TAPBP (TAP Binding Protein) is a Protein Coding gene. Diseases associated with TAPBP include Bare Lymphocyte Syndrome, Type I and Immunodeficiency By Defective Expression Of Mhc Class I. Among its related pathways are Antigen processing-Cross presentation and Innate Immune System. Gene Ontology (GO) annotations related to this gene include unfolded protein binding and MHC class I protein binding. An important paralog of this gene is TAPBPL.Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading).
Chromosomal Localization:
6p21.32
Accession Number:
XP_016866716.1
UniProt Accession Number:
O15533
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
IHHPSLPASGR
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
1210
Last Updated:
12/17/2020
Links
- EnsEMBL - EnsEMBL TAPBP
- Entrez - Entrez TAPBP
- Uniprot - Uniprot O15533
Characterization Data
SOPs
No SOPs available.
Don't have Adobe Reader™?
Get it for free at Adobe.com