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Sialic Acid Binding Ig Like Lectin 9 Peptide 1

Antibody: CPTC-SIGLEC9-1
Background

Catalog Number:

CPTC-SIGLEC9-1

RRID:

AB_2890092

Target Antigen:

Sialic Acid Binding Ig Like Lectin 9 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

05/17/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

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Characterization Data [Compare Characterization Data]
  Affinity Measurement by Biolayer Interferometry
Click to enlarge image Affinity and binding kinetics of CPTC-SIGLEC9-1 antibody and full length SIGLEC9 recombinant protein were measured using biolayer interferometry. CPTC-SIGLEC9 rabbit antibody was captured onto Protein A biosensors. The recombinant protein at 1024 nM, 256 nM, 64 nM, 16 nM, and 4 nM, was used as analyte. Buffer only and biosensors immobilized without antibody were used as references for background subtraction. All binding data was analyzed globally using a 1:1 fitting model. Click image to enlarge

CPTC-SIGLEC9-1 Affinity and Kinetics (Bio-Layer Interferometry) 

Result: Positive

Affinity and binding kinetics of CPTC-SIGLEC9-1 antibody and full length SIGLEC9 recombinant protein were measured using biolayer interferometry. CPTC-SIGLEC9 rabbit antibody was captured onto Protein A biosensors. The recombinant protein at 1024 nM, 256 nM, 64 nM, 16 nM, and 4 nM, was used as analyte. Buffer only and biosensors immobilized without antibody were used as references for background subtraction. All binding data was analyzed globally using a 1:1 fitting model.


Characterization SOP Files

  Affinity Measurement by SPR

CPTC-SIGLEC9-1 Affinity and Kinetics (Surface Plasmon Resonance)

Result: Negative

Affinity and binding kinetics of CPTC-SIGLEC9-1 and full length SIGLEC9 recombinant protein were measured using surface plasmon resonance. CPTC-SIGLEC9-1 antibody was captured on a protein A biosensor chip. SIGLEC9 recombinant protein at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM, and 0.25 nM, was used as analyte.


Characterization SOP Files

  IHC HPA
Download This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org). (497.6 KB)

CTPC-SIGLEC9-1 evaluation by the Human Protein Atlas

Result: Positive

This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Micro-Array (TMA) core of breast cancer showing cytoplasmic and membranous staining using Antibody CPTC-SIGLEC9-1. Titer: 1:1000 Click image to enlarge

CTPC-SIGLEC9-1 IHC Tissue

Result: Positive

Tissue Micro-Array (TMA) core of breast cancer showing cytoplasmic and membranous staining using Antibody CPTC-SIGLEC9-1. Titer: 1:1000


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (497.6 KB)

CTPC-SIGLEC9-1 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-SIGLEC9-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Click image to enlarge

CTPC-SIGLEC9-1 NCI60 Protein Array

Result: Positive

Protein Array in which CPTC-SIGLEC9-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-SIGLEC9-1 as primary antibody against human sialic acid binding Ig-like lectin 9 (SIGLEC9) recombinant protein (lane 2). Expected molecular weight - 49.9 kDa. Molecular weight standards are also included (lane 1). Target protein is subject to glycosylation which can affect the migration in electrophoresis. This can make the target appear as a higher molecular weight protein. Click image to enlarge

CPTC-SIGLEC9-1 Western Blot Recombinant Protein

Result: Positive

Western blot using CPTC-SIGLEC9-1 as primary antibody against human sialic acid binding Ig-like lectin 9 (SIGLEC9) recombinant protein (lane 2). Expected molecular weight - 49.9 kDa. Molecular weight standards are also included (lane 1). Target protein is subject to glycosylation which can affect the migration in electrophoresis. This can make the target appear as a higher molecular weight protein.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-SIGLEC9-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and NCI-H226 (lane 6) whole cell lysates. Expected molecular weight - 50.1 kDa and 52.5 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-SIGLEC9-1 Western Blot Cell Lysate

Result: Negative

Western blot using CPTC-SIGLEC9-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and NCI-H226 (lane 6) whole cell lysates. Expected molecular weight - 50.1 kDa and 52.5 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-SIGLEC9-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 50.1 kDa and 52.5 kDa. Molecular weight standards are also included (lane 1). All cell lines are positive. Target protein is subject to glycosylation which can affect the migration in electrophoresis. This can make the target appear as a higher molecular weight protein. Click image to enlarge

CPTC-SIGLEC9-1 Automated Western Blot Cell Lysate

Result: Positive

Automated western blot using CPTC-SIGLEC9-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 50.1 kDa and 52.5 kDa. Molecular weight standards are also included (lane 1). All cell lines are positive. Target protein is subject to glycosylation which can affect the migration in electrophoresis. This can make the target appear as a higher molecular weight protein.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-SIGLEC9-1 Western Blot (Tissue Lysates)

Result: Negative

Western blot using CPTC-SIGLEC9-1 as primary antibody against human breast (2), ovary (3), spleen (4), endometrium (5), and lung (6) tissue lysates. The expected molecular weight is 50.1 kDa. Cytochrome C was used as a loading control.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated WB (Simple Western) using CPTC-SIGLEC9-1 as primary antibody against the whole lysate of breast, ovary, spleen, endometrium and lung tissues. Expected MW is 50.1 KDa. The antibody recognizes the target in breast, ovary adn endometrium tissue lysates. The same cell lysates were probed with an anti-CytC antibody (bottom panel). Click image to enlarge

CPTC-SIGLEC9-1 Simple Western (tissue lysates)

Result: Positive

Automated WB (Simple Western) using CPTC-SIGLEC9-1 as primary antibody against the whole lysate of breast, ovary, spleen, endometrium and lung tissues. Expected MW is 50.1 KDa. The antibody recognizes the target in breast, ovary adn endometrium tissue lysates. The same cell lysates were probed with an anti-CytC antibody (bottom panel).


Characterization SOP Files

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Antigen: Sialic Acid Binding Ig Like Lectin 9 Peptide 1
Background

NCI Identification Number:

00410

Antigen Name:

Sialic Acid Binding Ig Like Lectin 9 Peptide 1

CPTC Name:

CPTC-SIGLEC9 Peptide 1

Aliases:

Sialic Acid Binding Ig Like Lectin 9; Sialic Acid-Binding Ig-Like Lectin 9; Protein FOAP-9; Siglec-9; CDw329; CD329; Sialic Acid Binding Ig-Like Lectin 9; CD329 Antigen; OBBP-LIKE; SIGLEC9; FOAP-9

Function:

IGLEC9 (Sialic Acid Binding Ig Like Lectin 9) is a Protein Coding gene. Among its related pathways are Innate Immune System and Class I MHC mediated antigen processing and presentation. Gene Ontology (GO) annotations related to this gene include carbohydrate binding. An important paralog of this gene is SIGLEC7.Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- or alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.

Chromosomal Localization:

19q13.41

Accession Number:

NP_001185487.1

UniProt Accession Number:

Q9Y336

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

LVCAVDAVDSNPPAR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1650

Last Updated:

09/03/2020

Links

Characterization Data

SOPs

No SOPs available.

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