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RB Transcriptional Corepressor 1 Peptide 4

Antibody: CPTC-RB1-6
Background

Catalog Number:

CPTC-RB1-6

RRID:

NA

Target Antigen:

RB Transcriptional Corepressor 1 Peptide 4

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

11/29/2022

Antigen Recognition(s):

Peptide

Characterization Data [Compare Characterization Data]
  Affinity Measurement by Biolayer Interferometry
Click to enlarge image The affinity and binding kinetics of CPTC-RB1-6 antibody and BSA-conjugated phosphorylated peptide “ISEGLP-pT-PTK" were measured using biolayer interferometry. BSA-conjugated peptide was covalently immobilized onto AR2G biosensors using standard amine coupling. CPTC-RB1-6 antibody, 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM and 0.25 nM, was used as analyte. Buffer only and biosensors immobilized without BSA-conjugated peptide were used as references for background subtraction. All data was analyzed globally using a 1:2 bivalent model. Click image to enlarge

CPTC-RB1-6 Affinity and Kinetics (Bio-Layer Interferometry)

Result: High Binding

The affinity and binding kinetics of CPTC-RB1-6 antibody and BSA-conjugated phosphorylated peptide “ISEGLP-pT-PTK" were measured using biolayer interferometry. BSA-conjugated peptide was covalently immobilized onto AR2G biosensors using standard amine coupling. CPTC-RB1-6 antibody, 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM and 0.25 nM, was used as analyte. Buffer only and biosensors immobilized without BSA-conjugated peptide were used as references for background subtraction. All data was analyzed globally using a 1:2 bivalent model.


Characterization SOP Files

  Affinity Measurement by SPR
Click to enlarge image The affinity and binding kinetics of CPTC-RB1-6 antibody and BSA-conjugated phosphorylated-peptide, “ISEGLP-pT-PTK” (A) and non-phosphorylated peptide (B), were measured using surface plasmon resonance. Peptide was amine coupled onto a Series S CM5 biosensor chip. Antibody at 64 nM, 16 nM, 4 nM, 1.0 nM, 0.25 nM and 0.0625 nM, was used as analyte. Binding data were double-referenced and analyzed globally using a bivalent fitting model. Click image to enlarge

CPTC-RB1-6 Affinity and Kinetics (Surface Plasmon Resonance) 

Result: High Binding

The affinity and binding kinetics of CPTC-RB1-6 antibody and BSA-conjugated phosphorylated-peptide, “ISEGLP-pT-PTK” (A) and non-phosphorylated peptide (B), were measured using surface plasmon resonance. Peptide was amine coupled onto a Series S CM5 biosensor chip. Antibody at 64 nM, 16 nM, 4 nM, 1.0 nM, 0.25 nM and 0.0625 nM, was used as analyte. Binding data were double-referenced and analyzed globally using a bivalent fitting model.


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM using CPTC-RB1-6 as capture antibody against the phosphorylated synthetic peptide ISEGLP(pT)PTK (phosphosite T821) and the correpondent non-phosphorylated peptide ISEGLPTPTK. Antibody CPTC-RB1-6 captures specifically the phosphorylated synthetic peptide, but not the correspondent non-phosphorylated peptide. Click image to enlarge

CPTC-RB1-6 iMRM (synthetic peptide)

Result: Positive

Immuno-MRM using CPTC-RB1-6 as capture antibody against the phosphorylated synthetic peptide ISEGLP(pT)PTK (phosphosite T821) and the correpondent non-phosphorylated peptide ISEGLPTPTK. Antibody CPTC-RB1-6 captures specifically the phosphorylated synthetic peptide, but not the correspondent non-phosphorylated peptide.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-RB1-6 as primary antibody against BSA conjugated phosphorylated peptide, "ISEGLP-pT-PTK", coated on the plate and detected using HRP conjugated goat anti-rabbit secondary antibody and TMB. Click image to enlarge

CPTC-RB1-6 Indirect ELISA

Result: High Binding

Indirect ELISA using CPTC-RB1-6 as primary antibody against BSA conjugated phosphorylated peptide, "ISEGLP-pT-PTK", coated on the plate and detected using HRP conjugated goat anti-rabbit secondary antibody and TMB.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-RB1-6 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide

CPTC-RB1-6 Western Blot (Recombinant Protein)

Result: Negative

Western blot using CPTC-RB1-6 as primary antibody against human Rb1 recombinant protein. The expected molecular weight is 108.0 kDa.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Automated western blot using CPTC-RB1-6 as primary antibody against recombinant RB1 protein. Protein molecular weight is about 108 KDa. Click image to enlarge

CPTC- RB1-6 Automated Western Blot (Recombinant Protein)

Result: Negative

Automated western blot using CPTC-RB1-6 as primary antibody against recombinant RB1 protein. Protein molecular weight is about 108 KDa.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-RB1-6 as primary antibody against whole cell lysates CCRF-CEM (lane 2), HeLa (lane 3), Jurkat (lane 4), K-562 (lane 5), and MCF7 (lane 6). The expected molecular weight is 106.2 kDa. All cell lines are negative. Click image to enlarge

CPTC-RB1-6 Western Blot (Cell Lysates)

Result: Negative

Western blot using CPTC-RB1-6 as primary antibody against whole cell lysates CCRF-CEM (lane 2), HeLa (lane 3), Jurkat (lane 4), K-562 (lane 5), and MCF7 (lane 6). The expected molecular weight is 106.2 kDa. All cell lines are negative.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-RB1-6 as primary antibody against whole lysates of cell lines CCRF-CEM, HeLa, Jurkat, K-562 and MCF7. Protein molecular weight is about 106 KDa. The antibody cannot recognize the target in any of the tested lysates. Loading controls were run with anti-GAPDH antibody. Click image to enlarge

CPTC-RB1-6 Automated Western Blot (Cell Lysates)

Result: Negative

Automated western blot using CPTC-RB1-6 as primary antibody against whole lysates of cell lines CCRF-CEM, HeLa, Jurkat, K-562 and MCF7. Protein molecular weight is about 106 KDa. The antibody cannot recognize the target in any of the tested lysates. Loading controls were run with anti-GAPDH antibody.


Characterization SOP Files

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Antigen: RB Transcriptional Corepressor 1 Peptide 4
Background

NCI Identification Number:

00529

Antigen Name:

RB Transcriptional Corepressor 1 Peptide 4

CPTC Name:

CPTC-RB1 Peptide 4

Aliases:

RB Transcriptional Corepressor 1; PPP1R130; RB; Protein Phosphatase 1, Regulatory Subunit 130; Prepro-Retinoblastoma-Associated Protein; Retinoblastoma 1; P110-RB1; P105-Rb; Pp110; PRb; Retinoblastoma Suspectibility Protein; Osteosarcoma; Rb; RB; Mutant Retinoblastoma-Associated Protein; Retinoblastoma Susceptibility Protein; Mutated Retinoblastoma Protein; Retinoblastoma; RB1 Protein; Rb

Function:

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma.RB1 (RB Transcriptional Corepressor 1) is a Protein Coding gene. Diseases associated with RB1 include Retinoblastoma and Familial Retinoblastoma. Among its related pathways are Phosphorylation of proteins involved in G1/S transition by active Cyclin E:Cdk2 complexes and SARS-CoV-1 Genome Replication and Transcription. An important paralog of this gene is RBL2.

Chromosomal Localization:

13q14.2

Accession Number:

UniProt Accession Number:

P06400

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

ISEGLP-pT-PTK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1100

Last Updated:

07/01/2026

Links

Characterization Data

SOPs

No SOPs available.

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