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Protein Tyrosine Phosphatase Non-Receptor Type 11 Peptide 2

Antibody: CPTC-PTPN11-2
Background

Catalog Number:

CPTC-PTPN11-2

Target Antigen:

Protein Tyrosine Phosphatase Non-Receptor Type 11 Peptide 2

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

11/29/2022

Antigen Recognition(s):

Peptide, Recombinant Full-length

Characterization Data [Compare Characterization Data]
  Affinity Measurement by Biolayer Interferometry
Click to enlarge image The affinity and binding kinetics of CPTC-PTPN11-2 antibody and BSA-conjugated phosphorylated peptide “GHE-pY-TNIK" were measured using biolayer interferometry. BSA-conjugated peptide was covalently immobilized onto AR2G biosensors using standard amine coupling. CPTC-PTPN11-2 antibody, 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM and 0.25 nM, was used as analyte. Buffer only and biosensors immobilized without BSA-conjugated peptide were used as references for background subtraction. All data was analyzed globally using a 1:2 bivalent model. Click image to enlarge

CPTC-PTPN11-2 Affinity and Kinetics (Bio-Layer Interferometry)

Result: High Binding

The affinity and binding kinetics of CPTC-PTPN11-2 antibody and BSA-conjugated phosphorylated peptide “GHE-pY-TNIK" were measured using biolayer interferometry. BSA-conjugated peptide was covalently immobilized onto AR2G biosensors using standard amine coupling. CPTC-PTPN11-2 antibody, 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM and 0.25 nM, was used as analyte. Buffer only and biosensors immobilized without BSA-conjugated peptide were used as references for background subtraction. All data was analyzed globally using a 1:2 bivalent model.


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM using CPTC-PTPN11-2 as capture antibody against the phosphorylated synthetic peptide GHE(pY)TNIK (phosphosite Y542) and the correspondent non-phosphorylated peptide GHEYTNIK. Antibody CPTC-PTPN11-2 captures specifically the phosphorylated synthetic peptide, but not the correspondent non-phosphorylated peptide. Click image to enlarge

CPTC-PTPN11-2 iMRM (synthetic peptide)

Result: Positive

Immuno-MRM using CPTC-PTPN11-2 as capture antibody against the phosphorylated synthetic peptide GHE(pY)TNIK (phosphosite Y542) and the correspondent non-phosphorylated peptide GHEYTNIK. Antibody CPTC-PTPN11-2 captures specifically the phosphorylated synthetic peptide, but not the correspondent non-phosphorylated peptide.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-PTPN11-2 as primary antibody against BSA conjugated phosphorylated peptide, "GHE-pY-TNIK", coated on the plate and detected using HRP conjugated goat anti-rabbit secondary antibody and TMB. Click image to enlarge

CPTC-PTPN11-2 Indirect ELISA

Result: High Binding

Indirect ELISA using CPTC-PTPN11-2 as primary antibody against BSA conjugated phosphorylated peptide, "GHE-pY-TNIK", coated on the plate and detected using HRP conjugated goat anti-rabbit secondary antibody and TMB.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-PTPN11-2 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide

CPTC-PTPN11-2 Western Blot (Recombinant Protein)

Result: Negative

Western blot using CPTC-PTPN11-2 as primary antibody against human protein tyrosine phosphatase, non-receptor type 11 (PTPN11) recombinant protein. The expected molecular weight is 67.8 kDa.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Automated western blot using CPTC-PTPN11-2 as primary antibody against recombinant PTPN11 protein. Protein molecular weight is about 68 KDa. The antibody recognizes recombinant PTPN11. Click image to enlarge

CPTC- PTPN11-2 Automated Western Blot (Recombinant Protein)

Result: Positive

Automated western blot using CPTC-PTPN11-2 as primary antibody against recombinant PTPN11 protein. Protein molecular weight is about 68 KDa. The antibody recognizes recombinant PTPN11.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-PTPN11-2 as primary antibody against whole cell lysates CCRF-CEM (lane 2), HeLa (lane 3), Jurkat (lane 4), K-562 (lane 5), and MCF7 (lane 6). The expected molecular weights are 68.0 kDa, 68.4 kDa, and 52.8 kDa. Click image to enlarge

CPTC-PTPN11-2 Western Blot (Cell Lysates)

Result: Negative

Western blot using CPTC-PTPN11-2 as primary antibody against whole cell lysates CCRF-CEM (lane 2), HeLa (lane 3), Jurkat (lane 4), K-562 (lane 5), and MCF7 (lane 6). The expected molecular weights are 68.0 kDa, 68.4 kDa, and 52.8 kDa.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-PTPN11-2 as primary antibody against whole lysates of cell lines CCRF-CEM, HeLa, Jurkat, K-562 and MCF7. Protein molecular weight is about 68 KDa. The antibody cannot recognize the target in any of the tested lysates. Loading controls were run with anti-GAPDH antibody. Click image to enlarge

CPTC-PTPN11-2 Automated Western Blot (Cell Lysates)

Result: Negative

Automated western blot using CPTC-PTPN11-2 as primary antibody against whole lysates of cell lines CCRF-CEM, HeLa, Jurkat, K-562 and MCF7. Protein molecular weight is about 68 KDa. The antibody cannot recognize the target in any of the tested lysates. Loading controls were run with anti-GAPDH antibody.


Characterization SOP Files

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Antigen: Protein Tyrosine Phosphatase Non-Receptor Type 11 Peptide 2
Background

NCI Identification Number:

00526

Antigen Name:

Protein Tyrosine Phosphatase Non-Receptor Type 11 Peptide 2

CPTC Name:

CPTC-PTPN11 Peptide 2

Aliases:

Protein Tyrosine Phosphatase Non-Receptor Type 11; SH-PTP2; PTP2C; BPTP3; SHP-2; SHP2; SH2 Domain-Containing Protein Tyrosine Phosphatase 2; Tyrosine-Protein Phosphatase Non-Receptor Type 11; Protein-Tyrosine Phosphatase 1D; Protein-Tyrosine Phosphatase 2C; EC 3.1.3.48; SH-PTP3; PTP-1D; NS1; Protein Tyrosine Phosphatase, Non-Receptor Type 11; Noonan Syndrome 1; METCDS; SHPTP2; PTP-2C; JMML; Shp2; CFC

Function:

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains two tandem Src homology-2 domains, which function as phospho-tyrosine binding domains and mediate the interaction of this PTP with its substrates. This PTP is widely expressed in most tissues and plays a regulatory role in various cell signaling events that are important for cell functions, such as mitogenic activation, metabolic control, transcription regulation, and cell migration. Mutations in this gene are a cause of Noonan syndrome as well as acute myeloid leukemia. [provided by RefSeq, Aug 2016]PTPN11 (Protein Tyrosine Phosphatase Non-Receptor Type 11) is a Protein Coding gene. Diseases associated with PTPN11 include Noonan Syndrome 1 and Juvenile Myelomonocytic Leukemia. Among its related pathways are SARS-CoV-2 Infection and Immune response IL-2 activation and signaling pathway. Gene Ontology (GO) annotations related to this gene include protein domain specific binding and protein tyrosine phosphatase activity. An important paralog of this gene is PTPN6.Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus (PubMed:10655584, PubMed:18559669, PubMed:18829466, PubMed:26742426, PubMed:28074573). Positively regulates MAPK signal transduction pathway (PubMed:28074573). Dephosphorylates GAB1, ARHGAP35 and EGFR (PubMed:28074573). Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulation of its RhoA binding activity (PubMed:18559669). Dephosphorylates CDC73 (PubMed:26742426). Dephosphorylates SOX9 on tyrosine residues, leading to inactivate SOX9 and promote ossification (By similarity). PTN11_HUMAN,Q06124Protein tyrosine phosphatases (PTPs) are a group of enzymes that catalyze the removal of phosphate groups from tyrosine residues by the hydrolysis of phosphoric acid monoesters. They directly oppose the actions of kinase and phosphorylase enzymes.

Chromosomal Localization:

12q24.13

Accession Number:

NP_002825.3

UniProt Accession Number:

Q06124

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

GHE-pY-TNIK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

880

Last Updated:

02/13/2025

Links

Characterization Data

SOPs

No SOPs available.

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