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Proteasome 20S Subunit Beta 10 Peptide 1

Antibody: CPTC-PSMB10-1
Background

Catalog Number:

CPTC-PSMB10-1

Target Antigen:

Proteasome 20S Subunit Beta 10 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

11/21/2023

Antigen Recognition(s):

Peptide, Recombinant Full-length

External Links
Characterization Data [Compare Characterization Data]
  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org) (506.1 KB)

CPTC-PSMB10-1 evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Micro-Array (TMA) core of colon cancer showing cytoplasmic staining using Antibody CPTC-PSMB10-1. Titer: 1:1000 Click image to enlarge

CPTC-PSMB10-1 IHC Tissue

Result: Positive

Tissue Micro-Array (TMA) core of colon cancer showing cytoplasmic staining using Antibody CPTC-PSMB10-1. Titer: 1:1000


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org) (506.1 KB)

CPTC-PSMB10-1 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  NCI 60 Protein Array

CPTC-PSMB10-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-PSMB10-1 as primary antibody against human PSMB10 (40-273, His-tag) recombinant protein (lane 2). Expected molecular weight - 26.9 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-PSMB10-1 Western Blot Recombinant Protein

Result: Positive

Western blot using CPTC-PSMB10-1 as primary antibody against human PSMB10 (40-273, His-tag) recombinant protein (lane 2). Expected molecular weight - 26.9 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-PSMB10-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 28.9 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-PSMB10-1 Automated Western Blot Cell Lysate

Result: Negative

Automated western blot using CPTC-PSMB10-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 28.9 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-PSMB10-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 28.9 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-PSMB10-1 Western Blot Cell Lysate

Result: Negative

Western blot using CPTC-PSMB10-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 28.9 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-PSMB10-1 as primary antibody against human lung (2), spleen (3), endometrium (4), breast (5), and ovary (6) tissue lysates. The expected molecular weight is 28.9 kDa. Vinculin was used as a loading control. Click image to enlarge

CPTC-PSMB10-1 Western Blot (Tissue Lysate)

Result: Negative

Western blot using CPTC-PSMB10-1 as primary antibody against human lung (2), spleen (3), endometrium (4), breast (5), and ovary (6) tissue lysates. The expected molecular weight is 28.9 kDa. Vinculin was used as a loading control.


Characterization SOP Files

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Antigen: Proteasome 20S Subunit Beta 10 Peptide 1
Background

NCI Identification Number:

00455

Antigen Name:

Proteasome 20S Subunit Beta 10 Peptide 1

CPTC Name:

CPTC-PSMB10 Peptide 1

Aliases:

Proteasome 20S Subunit Beta 10; LMP10; Proteasome (Prosome, Macropain) Subunit, Beta Type, 10; Multicatalytic Endopeptidase Complex Subunit MECl-1; Proteasome Subunit Beta Type-10; Low Molecular Mass Protein 10; Proteasome Subunit Beta 10; Proteasome Subunit Beta-2i; Macropain Subunit MECl-1; Proteasome MECl-1; EC 3.4.25.1; Beta2i; MECL1; Proteasome Catalytic Subunit 2i; Proteasome Subunit Beta 7i; Proteasome Subunit Beta2i; Proteasome Subunit MECL1; Proteasome Subunit β2i; MGC1665; PSMB10

Function:

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Proteolytic processing is required to generate a mature subunit. Expression of this gene is induced by gamma interferon, and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome.PSMB10 (Proteasome 20S Subunit Beta 10) is a Protein Coding gene. Diseases associated with PSMB10 include Myeloma, Multiple. Among its related pathways are Regulation of activated PAK-2p34 by proteasome mediated degradation and Antigen processing-Cross presentation. Gene Ontology (GO) annotations related to this gene include threonine-type endopeptidase activity. An important paralog of this gene is PSMB7.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides.

Chromosomal Localization:

16q22.1

Accession Number:

NP_002792.1

UniProt Accession Number:

P40306

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

LPFTALGSGQDAALAVLEDR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

2200

Last Updated:

10/01/2020

Links

Characterization Data

SOPs

No SOPs available.

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