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Perilipin 2

Antibody: CPTC-PLIN2-1
Background

Catalog Number:

CPTC-PLIN2-1

RRID:

AB_2617309

Target Antigen:

Perilipin 2

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

01/03/2024

Antigen Recognition(s):

Recombinant Full-length, Endogenous

Purchase
External Links
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (316.3 KB)

CPTC-PLIN2-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immunofluorescence - HPA
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line THP-1 shows localization to lipid droplets. Human assay: THP-1 fixed with PFA, dilution: 1:100 Human assay: U2OS fixed with PFA, dilution: 1:100 Click image to enlarge

CPTC-PLIN2-1 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line THP-1 shows localization to lipid droplets. Human assay: THP-1 fixed with PFA, dilution: 1:100
Human assay: U2OS fixed with PFA, dilution: 1:100


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-PLIN2-1 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Western Blot - HPA - tissue or cell lysate
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). Band of predicted size in kDa (+/-20%) with additional bands present. Analysis performed using a standard panel of samples. Antibody Dilution 1:500 Click image to enlarge

CPTC-PLIN2-1 Evaluation by the Human Protein Atlas

Result: Presumed Positive (with additional bands)

Results provided by the Human Protein Atlas (www.proteinatlas.org). Band of predicted size in kDa (+/-20%) with additional bands present.
Analysis performed using a standard panel of samples. Antibody Dilution 1:500


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-PLIN2-1 as primary Ab against PLIN2 (rAg 00092) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-PLIN2-1 Western blot

Result: Positive

Western Blot using CPTC-PLIN2-1 as primary Ab against PLIN2 (rAg 00092) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antibody: CPTC-PLIN2-2
Background

Catalog Number:

CPTC-PLIN2-2

RRID:

AB_2617310

Target Antigen:

Perilipin 2

Isotype:

IgG2a

Species:

Mouse Monoclonal Antibody

Last Updated:

01/04/2023

Antigen Recognition(s):

Recombinant Full-length, Endogenous

Purchase
External Links
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (306.9 KB)

CPTC-PLIN2-2 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immunofluorescence - HPA
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line U2OS shows localization to lipid droplets. Human assay: THP-1 fixed with PFA, dilution: 1:100 Human assay: U2OS fixed with PFA, dilution: 1:100 Click image to enlarge

CPTC-PLIN2-2 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line U2OS shows localization to lipid droplets. Human assay: THP-1 fixed with PFA, dilution: 1:100
Human assay: U2OS fixed with PFA, dilution: 1:100


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-PLIN2-2 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-PLIN2-2 as primary Ab against PLIN2 (rAg 00092) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-PLIN2-2 Western blot

Result: Positive

Western Blot using CPTC-PLIN2-2 as primary Ab against PLIN2 (rAg 00092) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antibody: CPTC-PLIN2-3
Background

Catalog Number:

CPTC-PLIN2-3

RRID:

AB_2617311

Target Antigen:

Perilipin 2

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

01/04/2023

Antigen Recognition(s):

Recombinant Full-length, Endogenous

Purchase
External Links
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (313.1 KB)

CPTC-PLIN2-3 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immunofluorescence - HPA
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line THP-1 shows localization to lipid droplets. Human assay: THP-1 fixed with PFA, dilution: 1:100 Human assay: U2OS fixed with PFA, dilution: 1:100 Click image to enlarge

CPTC-PLIN2-3 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line THP-1 shows localization to lipid droplets. Human assay: THP-1 fixed with PFA, dilution: 1:100
Human assay: U2OS fixed with PFA, dilution: 1:100


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-PLIN2-3 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-PLIN2-3 as primary Ab against PLIN2 (rAg 00092) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-PLIN2-3 Western blot

Result: Positive

Western Blot using CPTC-PLIN2-3 as primary Ab against PLIN2 (rAg 00092) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antigen: Perilipin 2
Background

NCI Identification Number:

00092

Antigen Name:

Perilipin 2

CPTC Name:

CPTC-PLIN2

Aliases:

Perilipin 2; Adipose Differentiation-Related Protein; ADFP; adipophilin; ADRP

Function:

The protein encoded by this gene belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene.

PLIN2 (perilipin 2) is a protein-coding gene. Diseases associated with PLIN2 include lipid-rich carcinoma, and kidney cancer. An important paralog of this gene is PLIN5.

May be involved in development and maintenance of adipose tissue.

Chromosomal Localization:

9p22.1

Expression System:

Baculovirus

Accession Number:

xx

UniProt Accession Number:

DNA Source:

N/A

Immunogen:

Recombinant Full Length Protein

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

MASVAVDPQPSVVTRVVNLPLVSSTYDLMSSAYLSTKDQYPYLKSVCEMA
ENGVKTITSV AMTSALPIIQKLEPQIAVANTYACKGLDRIEERLPILNQ
PSTQIVANAKGAVTGAKDAVT TTVTGAKDSVASTITGVMDKTKGAVTGS
VEKTKSVVSGSINTVLGSRMMQLVSSGVENAL TKSELLVEQYLPLTEEE
LEKEAKKVEGFDLVQKPSYYVRLGSLSTKLHSRAYQQALSRVK EAKQKS
QQTISQLHSTVHLIEFARKNVYSANQKIQDAQDKLYLSWVEWKRSIGYDD
TDES HCAEHIESRTLAIARNLTQQLQTTCHTLLSNIQGVPQNIQDQAKH
MGVMAGDIYSVFRNA ASFKEVSDSLLTSSKGQLQKMKESLDDVMDYLVN
NTPLNWLVGPFYPQLTESQNAQDQGA EMDKSSQETQRSEHKTH

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

48900

Last Updated:

03/05/2015

Links

Characterization Data

SOPs

No SOPs available.

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