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Macrophage Stimulating 1 Peptide 1

Antibody: CPTC-MST1-1
Background

Catalog Number:

CPTC-MST1-1

Target Antigen:

Macrophage Stimulating 1 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

02/09/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

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External Links
Characterization Data [Compare Characterization Data]
  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (320.7 KB)

CPTC-MST1 evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Micro-Array (TMA) core of lung cancer showing cytoplasmic staining using Antibody CPTC-MST1-1. Titer: 1:1000 Click image to enlarge

CPTC-MST1-1 IHC Tissue

Result: Positive

Tissue Micro-Array (TMA) core of lung cancer showing cytoplasmic staining using Antibody CPTC-MST1-1. Titer: 1:1000


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (320.7 KB)

CPTC-MST1 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  NCI 60 Protein Array

CPTC-MST1-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-MST1-1 as primary antibody against human macrophage stimulating 1 (hepatocyte growth factor-like) (MST1) recombinant protein (lane 2). Expected molecular weight - 78.4 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-MST1-1 Western Blot Recombinant Protein

Result: Positive

Western blot using CPTC-MST1-1 as primary antibody against human macrophage stimulating 1 (hepatocyte growth factor-like) (MST1) recombinant protein (lane 2). Expected molecular weight - 78.4 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-MST1-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 55.6 kDa and 52.3 kDa. Molecular weight standards are also included (lane 1). PBMC is positive. Jurkat and A549 are presumed positive. All other cell lines are negative. Click image to enlarge

CPTC-MST1-1 Automated Western Blot Cell Lysate

Result: Positive

Automated western blot using CPTC-MST1-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 55.6 kDa and 52.3 kDa. Molecular weight standards are also included (lane 1). PBMC is positive. Jurkat and A549 are presumed positive. All other cell lines are negative.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-MST1-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 80.3 kDa. Molecular weight standards are also included (lane 1). PBMC is presumed positive. All other cell lines are negative. Click image to enlarge

CPTC-MST1-1 Western Blot Cell Lysate

Result: Presumed Positive (with additional bands)

Western blot using CPTC-MST1-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 80.3 kDa. Molecular weight standards are also included (lane 1). PBMC is presumed positive. All other cell lines are negative.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-MST1-1 as primary antibody against human lung (2), spleen (3), endometrium (4), breast (5), and ovary (6) tissue lysates. The expected molecular weight is 55.6 kDa and 52.3 kDa. Vinculin was used as a loading control. Click image to enlarge

CPTC-MST1-1 Western Blot (Tissue Lysate)

Result: Negative

Western blot using CPTC-MST1-1 as primary antibody against human lung (2), spleen (3), endometrium (4), breast (5), and ovary (6) tissue lysates. The expected molecular weight is 55.6 kDa and 52.3 kDa. Vinculin was used as a loading control.


Characterization SOP Files

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Antigen: Macrophage Stimulating 1 Peptide 1
Background

NCI Identification Number:

00435

Antigen Name:

Macrophage Stimulating 1 Peptide 1

CPTC Name:

CPTC-MST1 Peptide 1

Aliases:

Macrophage Stimulating 1; MSP; Hepatocyte Growth Factor-Like Protein; Macrophage-Stimulating Protein; D3F15S2; DNF15S2; NF15S2; HGFL; Macrophage Stimulating 1 (Hepatocyte Growth Factor-Like); Hepatocyte Growth Factor-Like Protein Homolog; Macrophage Stimulatory Protein; Hepatocyte Growth Factor-Like; EC 3.4.21; MST1

Function:

The protein encoded by this gene contains four kringle domains and a serine protease domain, similar to that found in hepatic growth factor. Despite the presence of the serine protease domain, the encoded protein may not have any proteolytic activity. The receptor for this protein is RON tyrosine kinase, which upon activation stimulates ciliary motility of ciliated epithelial lung cells. This protein is secreted and cleaved to form an alpha chain and a beta chain bridged by disulfide bonds.MST1 (Macrophage Stimulating 1) is a Protein Coding gene. Diseases associated with MST1 include Cholangitis, Primary Sclerosing and Inflammatory Bowel Disease 12. Among its related pathways are Signaling by GPCR and amb2 Integrin signaling. Gene Ontology (GO) annotations related to this gene include serine-type endopeptidase activity. An important paralog of this gene is HGF.

Chromosomal Localization:

3p21.31

Accession Number:

NP_066278.3

UniProt Accession Number:

P26927

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

VVGGHPGNSPWTVSLR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1760

Last Updated:

09/28/2020

Links

Characterization Data

SOPs

No SOPs available.

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