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Multiple Inositol-Polyphosphate Phosphatase 1 Peptide 1

Antibody: CPTC-MINPP1-1
Background

Catalog Number:

CPTC-MINPP1-1

Target Antigen:

Multiple Inositol-Polyphosphate Phosphatase 1 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

02/09/2024

Antigen Recognition(s):

Peptide, Endogenous

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Characterization Data [Compare Characterization Data]
  Affinity Measurement by Biolayer Interferometry

CPTC-MINPP1-1 Affinity and Kinetics (Bio-Layer Interferometry)

Result: Negative

The affinity and binding kinetics of CPTC-MINPP1-1 antibody and full length human MINPP1 recombinant protein were measured using biolayer interferometry. Recombinant protein was covalently immobilized onto AR2G biosensors using standard amine coupling. Antibody at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM, and 0.25 nM, was used as analyte. Buffer only and biosensors immobilized without antibody were used as references for background subtraction. No binding was observed.


Characterization SOP Files

  Affinity Measurement by SPR

CPTC-MINPP1-1 Affinity and Kinetics (Surface Plasmon Resonance) 

Result: Negative

Affinity and binding kinetics of CPTC-MINPP1-1 and BSA conjugated MINPP1 peptide (MQRLFFHCFLSW) were measured using surface plasmon resonance. CPTC-MINPP1-1 antibody was captured onto a Series S Protein A biosensor chip. BSA conjugated peptide at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, and 1.0 nM was used as analyte. Binding data were double-referenced and analyzed globally.


Characterization SOP Files

  IHC Tissue

CPTC-MINPP1-1 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  IP
Click to enlarge image Immunoprecipitation using CPTC-MINPP1-1 as capture antibody against cell lysates of MCF7, HeLa, Jurkat and HepG2. Eluates were then tested with Simple Western (automated WB) using CPTC-MINPP1-2 as detection antibody (Panel A). Data were compared with Simple Western data obtained from the same cell lysates using CPTC-MINPP1-2 (Panel B). The antibody is able to pull down the target protein in all cell lysates, but weakly in HepG2. Click image to enlarge

CPTC-MINPP1-1 IP-WB

Result: Positive

Immunoprecipitation using CPTC-MINPP1-1 as capture antibody against cell lysates of MCF7, HeLa, Jurkat and HepG2. Eluates were then tested with Simple Western (automated WB) using CPTC-MINPP1-2 as detection antibody (Panel A). Data were compared with Simple Western data obtained from the same cell lysates using CPTC-MINPP1-2 (Panel B). The antibody is able to pull down the target protein in all cell lysates, but weakly in HepG2.


Characterization SOP Files

  Immunofluorescence

CPTC-MINPP1-1 Immunofluorescence

Result: Negative

Immunofluorescence staining using CPTC-MINPP1-1 as primary antibody against MCF7 cells shows no localization of MINPP1 protein.


Characterization SOP Files

  Indirect ELISA

CPTC-MINPP1-1 Indirect ELISA

Result: Negative

Indirect ELISA using CPTC-MINPP1-1 as primary rabbit antibody against BSA-conjugated peptide (MQRLFFHCFLSW) coated on the plate and detected using the goat anti-rabbit antibody and TMB. 


Characterization SOP Files

  NCI 60 Protein Array

CPTC-MINPP1-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Automated western blot using CPTC-MINPP1-1 antibody recombinant MINPP1 proteins (variant 1 and variant 2). The antibody did not recognize the recombinant proteins. Click image to enlarge

CPTC-MINNP1-1 Automated Western Blot (recombinant protein)

Result: Negative

Automated western blot using CPTC-MINPP1-1 antibody recombinant MINPP1 proteins (variant 1 and variant 2). The antibody did not recognize the recombinant proteins.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide

CPTC-MINNP1-1 Western Blot (recombinant protein)

Result: Negative

Automated western blot using CPTC-MINPP1-1 antibody recombinant MINPP1 proteins (variant 1 and variant 2). The antibody did not recognize the recombinant proteins.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Negative Automated western blot using CPTC-MINPP1-1 antibody against cell lysates of MCF7, HeLa, Jurkat and HepG2. The antibody is not able to detect the target in the tested cell lines. The same cell lines have been tested for Vinculin as loading control. Click image to enlarge

CPTC-MINNP1-1 Automated Western Blot (cell lysates)

Result: Negative

Negative
Automated western blot using CPTC-MINPP1-1 antibody against cell lysates of MCF7, HeLa, Jurkat and HepG2. The antibody is not able to detect the target in the tested cell lines. The same cell lines have been tested for Vinculin as loading control.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-MINPP1-1 antibody against cell lysates of MCF7, HeLa, Jurkat and HepG2. The antibody is presumably able to detect the target in the tested cell lines. The same cell lines have been tested for Vinculin as loading control. Click image to enlarge

CPTC-MINNP1-1 Western Blot (cell lysates)

Result: Presumed Positive (with additional bands)

Western blot using CPTC-MINPP1-1 antibody against cell lysates of MCF7, HeLa, Jurkat and HepG2. The antibody is presumably able to detect the target in the tested cell lines. The same cell lines have been tested for Vinculin as loading control.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-MINPP1-1 Single Cell Western Blot (Cell Lysate)

Result: Negative

Single cell western blot using CPTC-MINPP1-1 as a primary antibody against MCF7 cell lysate. MINPP1 protein expression was not detected.


Characterization SOP Files

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Antibody: CPTC-MINPP1-2
Background

Catalog Number:

CPTC-MINPP1-2

Target Antigen:

Multiple Inositol-Polyphosphate Phosphatase 1 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

02/09/2024

Antigen Recognition(s):

Peptide, Endogenous

Purchase
Characterization Data [Compare Characterization Data]
  Affinity Measurement by Biolayer Interferometry

CPTC-MINPP1-2 Affinity and Kinetics (Bio-Layer Interferometry)

Result: Negative

The affinity and binding kinetics of CPTC-MINPP1-2 antibody and full length human MINPP1 recombinant protein were measured using biolayer interferometry. Recombinant protein was covalently immobilized onto AR2G biosensors using standard amine coupling. Antibody at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM, and 0.25 nM, was used as analyte. Buffer only and biosensors immobilized without antibody were used as references for background subtraction. No binding was observed.


Characterization SOP Files

  Affinity Measurement by SPR

CPTC-MINPP1-2 Affinity and Kinetics (Surface Plasmon Resonance) 

Result: Negative

Affinity and binding kinetics of CPTC-MINPP1-2 and BSA conjugated MINPP1 peptide (MQRLFFHCFLSW) were measured using surface plasmon resonance. CPTC-MINPP1-2 antibody was captured onto a Series S Protein A biosensor chip. BSA conjugated peptide at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, and 1.0 nM was used as analyte. Binding data were double-referenced and analyzed globally.


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Micro-Array (TMA) core of colon cancer showing cytoplasmic and membranous staining using Antibody CPTC-MINPP1-2. Titer: 1:1000 Click image to enlarge

CPTC-MINPP1-2 IHC Tissue

Result: Positive

Tissue Micro-Array (TMA) core of colon cancer showing cytoplasmic and membranous staining using Antibody CPTC-MINPP1-2. Titer: 1:1000


Characterization SOP Files

  IP
Click to enlarge image Immunoprecipitation using CPTC-MINPP1-2 as capture antibody against cell lysates of MCF7, HeLa, Jurkat and HepG2. Eluates were then tested with Simple Western (automated WB) using CPTC-MINPP1-2 as detection antibody (Panel A). Data were compared with Simple Western data obtained from the same cell lysates using CPTC-MINPP1-2 (Panel B). The antibody is able to pull down the target protein in all cell lysates, but weakly in MCF7 and HepG2. Click image to enlarge

CPTC-MINPP1-2 IP-WB

Result: Positive

Immunoprecipitation using CPTC-MINPP1-2 as capture antibody against cell lysates of MCF7, HeLa, Jurkat and HepG2. Eluates were then tested with Simple Western (automated WB) using CPTC-MINPP1-2 as detection antibody (Panel A). Data were compared with Simple Western data obtained from the same cell lysates using CPTC-MINPP1-2 (Panel B). The antibody is able to pull down the target protein in all cell lysates, but weakly in MCF7 and HepG2.


Characterization SOP Files

  Immunofluorescence
Click to enlarge image Immunofluorescence staining using CPTC-MINPP1-2 as primary antibody against MCF7 cells. The CPTC-MINPP1-2 antibody presumably recognizes the MINPP1 protein in the cytoplasm (green). Click image to enlarge

CPTC-MINPP1-2 Immunofluorescence

Result: Presumed Positive

Immunofluorescence staining using CPTC-MINPP1-2 as primary antibody against MCF7 cells. The CPTC-MINPP1-2 antibody presumably recognizes the MINPP1 protein in the cytoplasm (green).


Characterization SOP Files

  Indirect ELISA

CPTC-MINPP1-2 Indirect ELISA

Result: Negative

Indirect ELISA using CPTC-MINPP1-2 as primary rabbit antibody against BSA-conjugated peptide (MQRLFFHCFLSW) coated on the plate and detected using the goat anti-rabbit antibody and TMB.


Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-MINPP1-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-MINPP1-2 NCI 60 Protein Array

Result: Positive

Protein Array in which CPTC-MINPP1-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Automated western blot using CPTC-MINPP1-2 antibody recombinant MINPP1 proteins (variant 1 and variant 2). The antibody did not recognize the recombinant proteins. Click image to enlarge

CPTC-MINNP1-2 Automated Western Blot (recombinant protein)

Result: Negative

Automated western blot using CPTC-MINPP1-2 antibody recombinant MINPP1 proteins (variant 1 and variant 2). The antibody did not recognize the recombinant proteins.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide

CPTC-MINNP1-2 Western Blot (recombinant protein)

Result: Negative

Western blot using CPTC-MINPP1-2 antibody recombinant MINPP1 proteins (variant 1 and variant 2). The antibody did not recognize the recombinant proteins.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-MINPP1-1 antibody against cell lysates of MCF7, HeLa, Jurkat and HepG2. The antibody is able to detect the target in the tested cell lines (weakly for HepG2). The same cell lines have been tested for Vinculin as loading control. Click image to enlarge

CPTC-MINNP1-1 Automated Western Blot (cell lysates)

Result: Positive

Automated western blot using CPTC-MINPP1-1 antibody against cell lysates of MCF7, HeLa, Jurkat and HepG2. The antibody is able to detect the target in the tested cell lines (weakly for HepG2). The same cell lines have been tested for Vinculin as loading control.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-MINPP1-2 Single Cell Western Blot (Cell Lysate)

Result: Negative

Single cell western blot using CPTC-MINPP1-2 as a primary antibody against MCF7 cell lysate. MINPP1 protein expression was not detected.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-MINPP1-1 antibody against cell lysates of MCF7, HeLa, Jurkat and HepG2. The antibody is presumably able to detect the target in the tested cell lines (weakly for HepG2). The same cell lines have been tested for Vinculin as loading control. Click image to enlarge

CPTC-MINPP1-2 western blot (cell lysates)

Result: Positive

Western blot using CPTC-MINPP1-1 antibody against cell lysates of MCF7, HeLa, Jurkat and HepG2. The antibody is presumably able to detect the target in the tested cell lines (weakly for HepG2). The same cell lines have been tested for Vinculin as loading control.


Characterization SOP Files

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Antigen: Multiple Inositol-Polyphosphate Phosphatase 1 Peptide 1
Background

NCI Identification Number:

00513

Antigen Name:

Multiple Inositol-Polyphosphate Phosphatase 1 Peptide 1

CPTC Name:

CPTC-MINPP1 Peptide 1

Aliases:

Multiple Inositol-Polyphosphate Phosphatase 1; MIPP; Multiple Inositol Polyphosphate Histidine Phosphatase, 1; Inositol (1,3,4,5)-Tetrakisphosphate 3-Phosphatase; Multiple Inositol Polyphosphate Phosphatase 1; 2,3-Bisphosphoglycerate 3-Phosphatase; Ins(1,3,4,5)P(4) 3-Phosphatase; 2,3-BPG Phosphatase; EC 3.1.3.62; Multiple Inositol Polyphosphate Phosphatase 2; EC 3.1.3.80; HIPER1; MINPP2; PCH16

Function:

This gene encodes multiple inositol polyphosphate phosphatase; an enzyme that removes 3-phosphate from inositol phosphate substrates. It is the only enzyme known to hydrolzye inositol pentakisphosphate and inositol hexakisphosphate. This enzyme also converts 2,3 bisphosphoglycerate (2,3-BPG) to 2-phosphoglycerate; an activity formerly thought to be exclusive to 2,3-BPG synthase/2-phosphatase (BPGM) in the Rapoport-Luebering shunt of the glycolytic pathway.MINPP1 (Multiple Inositol-Polyphosphate Phosphatase 1) is a Protein Coding gene. Diseases associated with MINPP1 include Thyroid Cancer, Nonmedullary, 2 and Pontocerebellar Hypoplasia, Type 16. Among its related pathways are Metabolism and superpathway of D-myo-inositol (1,4,5)-trisphosphate metabolism. Gene Ontology (GO) annotations related to this gene include phosphatase activity and protein histidine phosphatase activity.Acts as a phosphoinositide 5- and phosphoinositide 6-phosphatase and regulates cellular levels of inositol pentakisphosphate (InsP5) and inositol hexakisphosphate (InsP6). Also acts as a 2,3-bisphosphoglycerate 3-phosphatase, by mediating the dephosphorylation of 2,3-bisphosphoglycerate (2,3-BPG) to produce phospho-D-glycerate without formation of 3-phosphoglycerate. May play a role in bone development (endochondral ossification). May play a role in the transition of chondrocytes from proliferation to hypertrophy (By similarity).

Chromosomal Localization:

10q23.2

Accession Number:

NP_001171588.1

UniProt Accession Number:

Q9UNW1

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

MQRLFFHCFLSW

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1320

Last Updated:

03/22/2022

Links

Characterization Data

SOPs

No SOPs available.

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