Malate Dehydrogenase 1 NAD (soluble)


Background

Catalog Number:

CPTC-MDH1-1

RRID:

AB_10805146

Target Antigen:

Malate Dehydrogenase 1 NAD (soluble)

Isotype:

IgG2c

Species:

Mouse Monoclonal Antibody

Last Updated:

11/14/2019

Antigen Recognition(s):

Recombinant Full-length, Endogenous

Purchase
External Links
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (100.9 KB)

CPTC-MDH1-1

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


  IHC NCI60

CPTC-MDH1-1 IHC NCI60

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


  IHC Tissue

CPTC-MDH1-1 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

CPTC-MDH1-1 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)


  NCI 60 Protein Array

CPTC-MDH1-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


  Western Blot - Over-expressed transient protein in cell lysate
Click to enlarge image Western Blot using CPTC-MDH1-1 as primary Ab against cell lysate from transiently overexpressed HEK293T cells form Origene (lane 2). Also included are molecular wt. standards (lane 1), lysate from non-transfected HEK293T cells as neg control (lane 3) and recombinant Ag MDH1 (NCI 10990) in (lane 4). Click image to enlarge

CPTC-MDH1-1 Cell Lysate Western blot

Result: Positive

Western Blot using CPTC-MDH1-1 as primary Ab against cell lysate from transiently overexpressed HEK293T cells form Origene (lane 2). Also included are molecular wt. standards (lane 1), lysate from non-transfected HEK293T cells as neg control (lane 3) and recombinant Ag MDH1 (NCI 10990) in (lane 4).


  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-MDH1-1 as primary Ab against Ag 10990 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-MDH1-1 Western blot

Result: Positive

Western Blot using CPTC-MDH1-1 as primary Ab against Ag 10990 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Background

Catalog Number:

CPTC-MDH1-2

RRID:

AB_10804294

Target Antigen:

Malate Dehydrogenase 1 NAD (soluble)

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

11/14/2019

Antigen Recognition(s):

Recombinant Full-length, Endogenous

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (89.7 KB)

CPTC-MDH1-2 Evaluation by Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


  IHC NCI60

CPTC-MDH1-2 IHC NCI60

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


  IHC Tissue

CPTC-MDH1-2 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

CPTC-MDH1-2 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)


  NCI 60 Protein Array

CPTC-MDH1-2 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


  Western Blot - Over-expressed transient protein in cell lysate
Click to enlarge image Western Blot using CPTC-MDH1-2 as primary Ab against cell lysate from transiently overexpressed HEK293T cells form Origene (lane 2). Also included are molecular wt. standards (lane 1), lysate from non-transfected HEK293T cells as neg control (lane 3) and recombinant Ag MDH1 (NCI 10990) in (lane 4). Click image to enlarge

CPTC-MDH1-2 Cell Lysate Western blot

Result: Positive

Western Blot using CPTC-MDH1-2 as primary Ab against cell lysate from transiently overexpressed HEK293T cells form Origene (lane 2). Also included are molecular wt. standards (lane 1), lysate from non-transfected HEK293T cells as neg control (lane 3) and recombinant Ag MDH1 (NCI 10990) in (lane 4).


  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-MDH1-2 as primary Ab against Ag 10990 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-MDH1-2 Western blot

Result: Positive

Western Blot using CPTC-MDH1-2 as primary Ab against Ag 10990 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Background

Catalog Number:

CPTC-MDH1-3

RRID:

AB_10805425

Target Antigen:

Malate Dehydrogenase 1 NAD (soluble)

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

10/15/2010

Antigen Recognition(s):

Purchase
Characterization Data [Compare Characterization Data]
  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

CPTC-MDH1-3 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)


  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-MDH1-3 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-MDH1-3 Protein Array

Result: Positive

Protein Array in which CPTC-MDH1-3 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-MDH1-3 as primary Ab against Ag 10990 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-MDH1-3 Western blot

Result: Positive

Western Blot using CPTC-MDH1-3 as primary Ab against Ag 10990 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-MDH1-3 as primary Ab against cell lysate from transiently overexpressed HEK293T cells form Origene (lane 2). Also included are molecular wt. standards (lane 1), lysate from non-transfected HEK293T cells as neg control (lane 3) and recombinant Ag MDH1 (NCI 10990) in (lane 4). Click image to enlarge

CPTC-MDH1-3 Cell Lysate Western blot

Result: Positive

Western Blot using CPTC-MDH1-3 as primary Ab against cell lysate from transiently overexpressed HEK293T cells form Origene (lane 2). Also included are molecular wt. standards (lane 1), lysate from non-transfected HEK293T cells as neg control (lane 3) and recombinant Ag MDH1 (NCI 10990) in (lane 4).


Background

NCI Identification Number:

10990

Antigen Name:

Malate Dehydrogenase 1 NAD (soluble)

CPTC Name:

CPTC-MDH1

Aliases:

EC 1.1.1.37; MDH-s; MDHA; MGC:1375; MOR2

Function:

Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria.

Chromosomal Localization:

2p13.3

Expression System:

E. Coli

Accession Number:

BC001484.2

UniProt Accession Number:

P40925

DNA Source:

OB : MHS1011-169973

Immunogen:

Recombinant Full Length Protein

Vector Name:

pMCSG7

Extinction Coefficient:

32555

Buffers:

PBS

Expressed Sequence:

SNAMSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMM
GVLDGVLMELQDCALPLLKDVIATDKEDVAFKDLDVAILVGSMPRREGME
RKDLLKANVKIFKSQGAALDKYAKKSVKVIVVGNPANTNCLTASKSAPSI
PKENFSCLTRLDHNRAKAQIALKLGVTANDVKNVIIWGNHSSTQYPDVNH
AKVKLQGKEVGVYEALKDDSWLKGEFVTTVQQRGAAVIKARKLSSAMSAA
KAICDHVRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNKTW
KFVEGLPINDFSREKMDLTAKELTEEKESAFEFLSSA

Native Sequence:

MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVL
DGVLMELQDCALPLLKDVIATDKEDVAFKDLDVAILVGSMPRREGMERKD
LLKANVKIFKSQGAALDKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKE
NFSCLTRLDHNRAKAQIALKLGVTANDVKNVIIWGNHSSTQYPDVNHAKV
KLQGKEVGVYEALKDDSWLKGEFVTTVQQRGAAVIKARKLSSAMSAAKAI
CDHVRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNKTWKFV
EGLPINDFSREKMDLTAKELTEEKESAFEFLSSA

Calculated Isoelectric Point:

6.91

Molecular Weight:

36.43

Last Updated:

08/22/2020

Links

Characterization Data

Gel

Click to enlarge image PAGE of MDH1 (Ag 10990) in Lane 2 with molecular weight standards in lane 1
Click image to enlarge

CPTC-MDH1 PAGE

PAGE of MDH1 (Ag 10990) in Lane 2 with molecular weight standards in lane 1

Characterization SOP Files

SOPs

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