Catalog Number:
CPTC-MDH1-1
RRID:
AB_10805146
Target Antigen:
Malate Dehydrogenase 1 NAD (soluble)
Isotype:
IgG2c
Species:
Mouse Monoclonal Antibody
Last Updated:
11/14/2019
Antigen Recognition(s):
Recombinant Full-length, Endogenous
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Result: Positive
Western Blot using CPTC-MDH1-1 as primary Ab against cell lysate from transiently overexpressed HEK293T cells form Origene (lane 2). Also included are molecular wt. standards (lane 1), lysate from non-transfected HEK293T cells as neg control (lane 3) and recombinant Ag MDH1 (NCI 10990) in (lane 4).
Result: Positive
Western Blot using CPTC-MDH1-1 as primary Ab against Ag 10990 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Catalog Number:
CPTC-MDH1-2
RRID:
AB_10804294
Target Antigen:
Malate Dehydrogenase 1 NAD (soluble)
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
11/14/2019
Antigen Recognition(s):
Recombinant Full-length, Endogenous
Result: Positive
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Result: Positive
Western Blot using CPTC-MDH1-2 as primary Ab against cell lysate from transiently overexpressed HEK293T cells form Origene (lane 2). Also included are molecular wt. standards (lane 1), lysate from non-transfected HEK293T cells as neg control (lane 3) and recombinant Ag MDH1 (NCI 10990) in (lane 4).
Result: Positive
Western Blot using CPTC-MDH1-2 as primary Ab against Ag 10990 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Catalog Number:
CPTC-MDH1-3
RRID:
AB_10805425
Target Antigen:
Malate Dehydrogenase 1 NAD (soluble)
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
10/15/2010
Antigen Recognition(s):
Result: Positive
Indirect ELISA (ie, binding of Antibody to Antigen coated plate)
Result: Positive
Protein Array in which CPTC-MDH1-3 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).
Result: Positive
Western Blot using CPTC-MDH1-3 as primary Ab against Ag 10990 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).
Result: Positive
Western Blot using CPTC-MDH1-3 as primary Ab against cell lysate from transiently overexpressed HEK293T cells form Origene (lane 2). Also included are molecular wt. standards (lane 1), lysate from non-transfected HEK293T cells as neg control (lane 3) and recombinant Ag MDH1 (NCI 10990) in (lane 4).
NCI Identification Number:
10990
Antigen Name:
Malate Dehydrogenase 1 NAD (soluble)
CPTC Name:
CPTC-MDH1
Aliases:
EC 1.1.1.37; MDH-s; MDHA; MGC:1375; MOR2
Function:
Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria.
Chromosomal Localization:
2p13.3
Expression System:
E. Coli
Accession Number:
BC001484.2
UniProt Accession Number:
P40925
DNA Source:
OB : MHS1011-169973
Immunogen:
Recombinant Full Length Protein
Vector Name:
pMCSG7
Extinction Coefficient:
32555
Buffers:
PBS
Expressed Sequence:
SNAMSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMM
GVLDGVLMELQDCALPLLKDVIATDKEDVAFKDLDVAILVGSMPRREGME
RKDLLKANVKIFKSQGAALDKYAKKSVKVIVVGNPANTNCLTASKSAPSI
PKENFSCLTRLDHNRAKAQIALKLGVTANDVKNVIIWGNHSSTQYPDVNH
AKVKLQGKEVGVYEALKDDSWLKGEFVTTVQQRGAAVIKARKLSSAMSAA
KAICDHVRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNKTW
KFVEGLPINDFSREKMDLTAKELTEEKESAFEFLSSA
Native Sequence:
MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVL
DGVLMELQDCALPLLKDVIATDKEDVAFKDLDVAILVGSMPRREGMERKD
LLKANVKIFKSQGAALDKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKE
NFSCLTRLDHNRAKAQIALKLGVTANDVKNVIIWGNHSSTQYPDVNHAKV
KLQGKEVGVYEALKDDSWLKGEFVTTVQQRGAAVIKARKLSSAMSAAKAI
CDHVRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNKTWKFV
EGLPINDFSREKMDLTAKELTEEKESAFEFLSSA
Calculated Isoelectric Point:
6.91
Molecular Weight:
36.43
Last Updated:
08/22/2020
PAGE of MDH1 (Ag 10990) in Lane 2 with molecular weight standards in lane 1
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