Mitogen-Activated Protein Kinase 3 Peptide 6


Background

Catalog Number:

CPTC-MAPK3-4

RRID:

AB_2827841

Target Antigen:

Mitogen-Activated Protein Kinase 3 Peptide 6

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

08/22/2022

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous, Phosphorylation

Thematic Panel(s):

Ras Pathway

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Publications
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org). (420.0 KB)

CPTC-MAPK3-4 evaluation by the Human Protein Atlas

Result: Negative

This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org).


  IHC Tissue
Click to enlarge image Tissue Microarray core of breast cancer immunohistochemically stained with antibody CPTC-MAPK3-4. Click image to enlarge

CPTC-MAPK3-4

Result: Positive

Tissue Microarray core of breast cancer immunohistochemically stained with antibody CPTC-MAPK3-4.


  Immuno-MRM
Click to enlarge image iMRM screening results for clone CPTC-MAPK3-5. The clone is able to pull down not only the target peptide (panel 1, CPTC-MAPK3 Peptide 6, IADPEHDHTGFL(pT)EYVATR), but also the following peptides: 
CPTC-MAPK3 Peptide 7 (IADPEHDHTGFL(pT)E(pY)VATR, panel 2)
CPTC-MAPK3 Peptide 1 (IADPEHDHTGFLTEYVATR, panel 3)
CPTC-MAPK1 Peptide 2 (VADPDHDHTGFL(pT)EYVATR, panel 4)
CPTC-MAPK1 Peptide 3 (VADPDHDHTGFLTE(pY)VATR, panel 5)
MAPK1 non-phosohorylated peptide (VADPDHDHTGFLTEYVATR, panel 6).

Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html) Click image to enlarge

CPTC-MAPK3-4 iMRM (synthetic peptide)

Result: Positive

iMRM screening results for clone CPTC-MAPK3-5. The clone is able to pull down not only the target peptide (panel 1, CPTC-MAPK3 Peptide 6, IADPEHDHTGFL(pT)EYVATR), but also the following peptides:
CPTC-MAPK3 Peptide 7 (IADPEHDHTGFL(pT)E(pY)VATR, panel 2)
CPTC-MAPK3 Peptide 1 (IADPEHDHTGFLTEYVATR, panel 3)
CPTC-MAPK1 Peptide 2 (VADPDHDHTGFL(pT)EYVATR, panel 4)
CPTC-MAPK1 Peptide 3 (VADPDHDHTGFLTE(pY)VATR, panel 5)
MAPK1 non-phosohorylated peptide (VADPDHDHTGFLTEYVATR, panel 6).

Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html)


  Immunofluorescence

CPTC-MAPK3-4 Immunofluorescence

Result: Negative

Immunofluorescence staining of MCF10a cells stably transfected with KRAS (MCF10a-KRAS) using CPTC-MAPK3-4 antibody. MAPK3 protein expression was not detected.


  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-MAPK3-4 as primary Ab against recombinant MAPK3 (lane 2). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-MAPK3-4 Western Blot

Result: Positive

Western Blot using CPTC-MAPK3-4 as primary Ab against recombinant MAPK3 (lane 2). Also included are molecular wt. standards (lane 1).


  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-MAPK3-4 as primary Ab against MCF10A-KRAS cell lysate (lane 2). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-MAPK3-4 Cell Lysate Western Blot

Result: Positive

Western Blot using CPTC-MAPK3-4 as primary Ab against MCF10A-KRAS cell lysate (lane 2). Also included are molecular wt. standards (lane 1).


Background

Catalog Number:

CPTC-MAPK3-5

RRID:

AB_2827842

Target Antigen:

Mitogen-Activated Protein Kinase 3 Peptide 6

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

07/14/2022

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous, Phosphorylation

Thematic Panel(s):

Ras Pathway

Purchase
Publications
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (310.6 KB)

CPTC-MAPK3-5 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


  Immuno-MRM
Click to enlarge image iMRM screening results for clone CPTC-MAPK3-5. The clone is able to pull down not only the target peptide (panel 1, CPTC-MAPK3 Peptide 6, IADPEHDHTGFL(pT)EYVATR), but also the following peptides: 
CPTC-MAPK3 Peptide 7 (IADPEHDHTGFL(pT)E(pY)VATR, panel 2)
CPTC-MAPK1 Peptide 2 (VADPDHDHTGFL(pT)EYVATR, panel 3)
CPTC-MAPK1 Peptide 1 (VADPDHDHTGFL(pT)E(pY)VATR, panel 4)

Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html) Click image to enlarge

CPTC-MAPK3-5 iMRM (synthetic peptide)

Result: Positive

iMRM screening results for clone CPTC-MAPK3-5. The clone is able to pull down not only the target peptide (panel 1, CPTC-MAPK3 Peptide 6, IADPEHDHTGFL(pT)EYVATR), but also the following peptides:
CPTC-MAPK3 Peptide 7 (IADPEHDHTGFL(pT)E(pY)VATR, panel 2)
CPTC-MAPK1 Peptide 2 (VADPDHDHTGFL(pT)EYVATR, panel 3)
CPTC-MAPK1 Peptide 1 (VADPDHDHTGFL(pT)E(pY)VATR, panel 4)

Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html)


  Immunofluorescence

CPTC-MAPK3-5 Immunofluorescence

Result: Negative

Immunofluorescence staining of MCF10a cells stably transfected with KRAS (MCF10a-KRAS) using CPTC-MAPK3-5 antibody. MAPK3 protein expression was not detected.


  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-MAPK3-5 as primary Ab against recombinant MAPK3 (lane 2). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-MAPK3-5 Western Blot

Result: Positive

Western Blot using CPTC-MAPK3-5 as primary Ab against recombinant MAPK3 (lane 2). Also included are molecular wt. standards (lane 1).


  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-MPK3-5 as primary Ab against MCF10A-KRAS cell lysate (lane 2). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-MAPK3-5 Cell Lysate Western Blot

Result: Positive

Western Blot using CPTC-MPK3-5 as primary Ab against MCF10A-KRAS cell lysate (lane 2). Also included are molecular wt. standards (lane 1).


Background

NCI Identification Number:

00282

Antigen Name:

Mitogen-Activated Protein Kinase 3 Peptide 6

CPTC Name:

CPTC-MAPK3 Peptide 6

Aliases:

Mitogen-Activated Protein Kinase 3; Extracellular Signal-Regulated Kinase 1; Microtubule-Associated Protein 2 Kinase; Insulin-Stimulated MAP2 Kinase; MAP Kinase Isoform P44; EC 2.7.11.24; P44-ERK1; P44-MAPK; PRKM3; ERK-1; ERK1; ERT2; Extracellular Signal-Related Kinase 1; MAP Kinase 3; EC 2.7.11; HS44KDAP; HUMKER1A; P44ERK1; P44MAPK; MAPK 1; MAPK 3

Function:

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described.

MAPK3 (Mitogen-Activated Protein Kinase 3) is a Protein Coding gene. Diseases associated with MAPK3 include Small Intestine Neuroendocrine Neoplasm and Adrenal Cortical Adenocarcinoma. Among its related pathways are GDNF-Family Ligands and Receptor Interactions and p70S6K Signaling. GO annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is MAPK1.

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade.

Extracellular signal-regulated kinases are a group of mitogen-activated protein kinases (MAPK) that mediate intracellular signaling. ERK1 and ERK2 (MAPK3 and MAPK1) transduce signals from growth factors and phorbol esters. They are expressed in all tissues, at varying levels.

Chromosomal Localization:

16p11.2

Accession Number:

NP_001035145.1

UniProt Accession Number:

P27361

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

IADPEHDHTGFL(pT)EYVATR

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

2090

Last Updated:

01/03/2019

Links

Characterization Data

SOPs

No SOPs available.

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