PRINT

Mitogen-Activated Protein Kinase 1 Peptide 1

Antibody: CPTC-MAPK1-1
Background

Catalog Number:

CPTC-MAPK1-1

RRID:

AB_2827852

Target Antigen:

Mitogen-Activated Protein Kinase 1 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

07/26/2023

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous, Phosphorylation

Thematic Panel(s):

Ras Pathway

Purchase
Publications
Characterization Data [Compare Characterization Data]
  Affinity Measurement by Biolayer Interferometry

CPTC-MAPK1-1 Affinity and Kinetics (Bio-Layer Interferometry) 

Result: Negative

Affinity and binding kinetics of CPTC-MAPK1-1 antibody and full-length MAPK1 recombinant protein were measured using biolayer interferometry. MAPK1 full length recombinant protein was amine coupled onto AR2G biosensors. CPTC-MAPK1-1 antibody was used as analyte and was titrated at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM, and 0.25 nM. Buffer only and biosensors having no recombinant protein were used as references for background subtraction. All binding data were analyzed globally.


Characterization SOP Files

  Affinity Measurement by SPR

CPTC-MAPK1-1 Affinity and Kinetics (Surface Plasmon Resonance)

Result: Negative

Affinity and binding kinetics of CPTC-MAPK1-1 and full-length MAPK1 recombinant protein were measured using surface plasmon resonance. MAPK1 recombinant protein was amine coupled onto a Series S CM5 biosensor chip. CPTC-MAPK1-1 antibody was used as analyte and was titrated at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM, 0.25 nM and 0.625 nM. All binding data were double-referenced and analyzed globally using a 1:2 bivalent fitting model. 


Characterization SOP Files

  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (465.2 KB)

CPTC-MAPK1-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immuno-MRM
Click to enlarge image iMRM screening results for clone CPTC-MAPK1-1. The clone is able to pull down not only the target peptide (panel 1, CPTC-MAPK1 Peptide 1, VADPDHDHTGFL(pT)E(pY)VATR), but also the following peptides: CPTC-MAPK1 Peptide 2 (VADPDHDHTGFL(pT)EYVATR, panel 2) CPTC-MAPK1 Peptide 3 (VADPDHDHTGFLTE(pY)VATR, panel 3) MAPK1 non-phosphorylated peptide (VADPDHDHTGFLTEYVATR, panel 4) CPTC-MAPK3 Peptide 1 (IADPEHDHTGFLTEYVATR, panel 5) CPTC-MAPK3 Peptide 6 (IADPEHDHTGFL(pT)EYVATR, panel 6) CPTC-MAPK3 Peptide 7 (IADPEHDHTGFLTE(pY)VATR, panel 7) MAPK3 double phosphorylated peptide (IADPEHDHTGFL(pT)E(pY)VATR, panel 8). Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html) Click image to enlarge

CPTC-MAPK1-1 iMRM (synthetic peptide)

Result: Positive

iMRM screening results for clone CPTC-MAPK1-1. The clone is able to pull down not only the target peptide (panel 1, CPTC-MAPK1 Peptide 1, VADPDHDHTGFL(pT)E(pY)VATR), but also the following peptides:
CPTC-MAPK1 Peptide 2 (VADPDHDHTGFL(pT)EYVATR, panel 2)
CPTC-MAPK1 Peptide 3 (VADPDHDHTGFLTE(pY)VATR, panel 3)
MAPK1 non-phosphorylated peptide (VADPDHDHTGFLTEYVATR, panel 4)
CPTC-MAPK3 Peptide 1 (IADPEHDHTGFLTEYVATR, panel 5)
CPTC-MAPK3 Peptide 6 (IADPEHDHTGFL(pT)EYVATR, panel 6)
CPTC-MAPK3 Peptide 7 (IADPEHDHTGFLTE(pY)VATR, panel 7)
MAPK3 double phosphorylated peptide (IADPEHDHTGFL(pT)E(pY)VATR, panel 8).

Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html)


Characterization SOP Files

  Immunofluorescence

CPTC-MAPK1-1 Immunofluorescence

Result: Negative

Immunofluorescence staining of MCF10a cells stably transfected with KRAS (MCF10a-KRAS) using CPTC- MAPK1-1 antibody. MAPK1 protein expression was not detected.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-MAPK1-1 antibody as primary rabbit antibody against full length recombinant MAPK1 protein. MAPK1 recombinant protein was coated on the plate and detected using goat anti-rabbit antibody and TMB. Click image to enlarge

CPTC-MAPK1-1 Indirect ELISA

Result: Positive

Indirect ELISA using CPTC-MAPK1-1 antibody as primary rabbit antibody against full length recombinant MAPK1 protein. MAPK1 recombinant protein was coated on the plate and detected using goat anti-rabbit antibody and TMB.


Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-MAPK1-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-MAPK1-1 NCI 60 Protein Array

Result: Positive

Protein Array in which CPTC-MAPK1-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-MAPK1-1 as primary Ab against recombinant MAPK1 (lane 2). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-MAPK1-1 Western Blot

Result: Positive

Western Blot using CPTC-MAPK1-1 as primary Ab against recombinant MAPK1 (lane 2). Also included are molecular wt. standards (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-MAPK1-1 as primary Ab against MCF10A-KRAS cell lysate (lane 2). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-MAPK1-1 Cell Lysate Western Blot

Result: Positive

Western Blot using CPTC-MAPK1-1 as primary Ab against MCF10A-KRAS cell lysate (lane 2). Also included are molecular wt. standards (lane 1).


Characterization SOP Files

[top]

Antibody: CPTC-MAPK1-2
Background

Catalog Number:

CPTC-MAPK1-2

RRID:

AB_2827853

Target Antigen:

Mitogen-Activated Protein Kinase 1 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

11/21/2023

Antigen Recognition(s):

Peptide, Phosphorylation

Thematic Panel(s):

Ras Pathway

Purchase
Publications
Characterization Data [Compare Characterization Data]
  Affinity Measurement by Biolayer Interferometry

CPTC-MAPK1-2 Affinity and Kinetics (Bio-Layer Interferometry)

Result: Negative

Affinity and binding kinetics of CPTC-MAPK1-2 antibody and full-length MAPK1 recombinant protein were measured using biolayer interferometry. MAPK1 full length recombinant protein was amine coupled onto AR2G biosensors. CPTC-MAPK1-2 antibody was used as analyte and was titrated at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM, and 0.25 nM. Buffer only and biosensors having no recombinant protein were used as references for background subtraction. All binding data were analyzed globally.


Characterization SOP Files

  Affinity Measurement by SPR

CPTC-MAPK1-2 Affinity and Kinetics (Surface Plasmon Resonance)

Result: Negative

Affinity and binding kinetics of CPTC-MAPK1-2 and full-length MAPK1 recombinant protein were measured using surface plasmon resonance. MAPK1 recombinant protein was amine coupled onto a Series S CM5 biosensor chip. CPTC-MAPK1-2 antibody was used as analyte and was titrated at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM, 0.25 nM and 0.625 nM. All binding data were double-referenced and analyzed globally using a 1:2 bivalent fitting model.  


Characterization SOP Files

  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (318.7 KB)

CPTC-MAPK1-2 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immuno-MRM
Click to enlarge image iMRM screening results for clone CPTC-MAPK1-2. The clone is able to pull down not only the target peptide (panel 1, CPTC-MAPK1 Peptide 1, VADPDHDHTGFL(pT)E(pY)VATR) and peptide IADPEHDHTGFL(pT)E(pY)VATR (panel 2). Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html) Click image to enlarge

CPTC-MAPK1-2 iMRM (synthetic peptide)

Result: Positive

iMRM screening results for clone CPTC-MAPK1-2. The clone is able to pull down not only the target peptide (panel 1, CPTC-MAPK1 Peptide 1, VADPDHDHTGFL(pT)E(pY)VATR) and peptide IADPEHDHTGFL(pT)E(pY)VATR (panel 2).

Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html)


Characterization SOP Files

  Indirect ELISA

CPTC-MAPK1-2 Indirect ELISA

Result: Negative

Indirect ELISA using CPTC-MAPK1-2 antibody as primary rabbit antibody against full length recombinant MAPK1 protein. MAPK1 protein is coated on the plate and detected using goat anti-rabbit antibody and TMB.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-MAPK1-2 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

[top]

Antigen: Mitogen-Activated Protein Kinase 1 Peptide 1
Background

NCI Identification Number:

00272

Antigen Name:

Mitogen-Activated Protein Kinase 1 Peptide 1

CPTC Name:

CPTC-MAPK1 Peptide 1

Aliases:

Mitogen-Activated Protein Kinase 1; Extracellular Signal-Regulated Kinase 2; Mitogen-Activated Protein Kinase 2; MAP Kinase Isoform P42; MAP Kinase 1; MAP Kinase 2; P42-MAPK; MAPK 2; PRKM1; PRKM2; ERK-2; ERK2; ERT1; Protein Tyrosine Kinase ERK2; EC 2.7.11.24; P42MAPK; P41mapk; MAPK 1; ERK; P38; P40; P41

Function:

This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.

MAPK1 (Mitogen-Activated Protein Kinase 1) is a Protein Coding gene. Diseases associated with MAPK1 include Chromosome 22Q11.2 Deletion Syndrome, Distal and Pertussis. Among its related pathways are ERK Signaling and Focal Adhesion. Gene Ontology (GO) annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is MAPK3.

erine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DCC, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. Mediates phosphorylation of TPR in respons to EGF stimulation. May play a role in the spindle assembly checkpoint. Phosphorylates PML and promotes its interaction with PIN1, leading to PML degradation. Phosphorylates CDK2AP2 (By similarity).

Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.

Extracellular signal-regulated kinases are a group of mitogen-activated protein kinases (MAPK) that mediate intracellular signaling. ERK1 and ERK2 (MAPK3 and MAPK1) transduce signals from growth factors and phorbol esters. They are expressed in all tissues, at varying levels.

Chromosomal Localization:

22q11.22

Accession Number:

NP_002736.3

UniProt Accession Number:

P28482

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

VADPDHDHTGFL(pT)E(pY)VATR

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

2090

Last Updated:

10/01/2019

Links

Characterization Data

SOPs

No SOPs available.

[top]

Don't have Adobe Reader™?

Get it for free at Adobe.com

  [top]