PRINT

Lck Interacting Transmembrane Adaptor 1 Peptide 1

Antibody: CPTC-LIME1-1
Background

Catalog Number:

CPTC-LIME1-1

Target Antigen:

Lck Interacting Transmembrane Adaptor 1 Peptide 1

Isotype:

IgG

Species:

Rabbit Recombinant Cloned Antibody

Last Updated:

08/26/2022

Antigen Recognition(s):

Peptide, Phosphorylation

Thematic Panel(s):

Immuno-Oncology (IO)

Publications
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (300.0 KB)

CPTC-LIME1-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC Tissue

CTPC-LIME1-1 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Western Blot - Over-expressed transient protein in cell lysate

CPTC-LIME1-1 Western Blot Over-Expressed Lysate

Result: Negative

Western blot using CPTC-LIME1-1 as primary antibody against transient overexpression lysate of human Lck interacting transmembrane adaptor 1 (LIME1) (lane 2). Expected molecular weight - 31.3 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-LIME1-1 as primary antibody against cell lysates HeLa, MCF10A, and LCL57. Samples from each cell line were irradiated with 10 Gy as shown in ‘+’ indicated lanes. Samples from each non-irradiated cell line were treated with alkaline phosphatase enzyme as shown in ‘-‘ indicated lanes. Molecular weight standards are included for each cell line. Click image to enlarge

CPTC-LIME1-1 automated western blot (cell lysate)

Result: Negative

Automated western blot using CPTC-LIME1-1 as primary antibody against cell lysates HeLa, MCF10A, and LCL57. Samples from each cell line were irradiated with 10 Gy as shown in ‘+’ indicated lanes. Samples from each non-irradiated cell line were treated with alkaline phosphatase enzyme as shown in ‘-‘ indicated lanes. Molecular weight standards are included for each cell line.


Characterization SOP Files

[top]

Antigen: Lck Interacting Transmembrane Adaptor 1 Peptide 1
Background

NCI Identification Number:

00299

Antigen Name:

Lck Interacting Transmembrane Adaptor 1 Peptide 1

CPTC Name:

CPTC-LIME1 Peptide 1

Aliases:

Lck Interacting Transmembrane Adaptor 1; Lck-Interacting Membrane Protein; Lck-Interacting Molecule; LIME; DJ583P15.4

Function:

This gene encodes a transmembrane adaptor protein that links the T and B-cell receptor stimulation to downstream signaling pathways via its association with the Src family kinases Lck and Lyn, respectively. Alternative splicing of this gene results in multiple transcript variants. LIME1 (Lck Interacting Transmembrane Adaptor 1) is a Protein Coding gene. An important paralog of this gene is ENSG00000273047.Involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and TCR (T-cell antigen receptor)-mediated T-cell signaling in T-cells. In absence of TCR signaling, may be involved in CD4-mediated inhibition of T-cell activation. Couples activation of these receptors and their associated kinases with distal intracellular events such as calcium mobilization or MAPK activation through the recruitment of PLCG2, GRB2, GRAP2, and other signaling molecules.

Chromosomal Localization:

20q13.33

Accession Number:

NP_060276.2

UniProt Accession Number:

Q9H400

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

CSSTC(Cam)GAGT(ph)PPASSC(Cam)PSLGR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

2200

Last Updated:

08/21/2020

Links

Characterization Data

SOPs

No SOPs available.

[top]

Don't have Adobe Reader™?

Get it for free at Adobe.com

  [top]