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Integrin Subunit Alpha X Peptide 1

Antibody: CPTC-ITGAX-1
Background

Catalog Number:

CPTC-ITGAX-1

Target Antigen:

Integrin Subunit Alpha X Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

02/06/2024

Antigen Recognition(s):

Peptide, Endogenous

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External Links
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (381.0 KB)

CPTC-ITGAX-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC Tissue

CPTC-ITGAX-1 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Immunofluorescence - HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (381.0 KB)

CPTC-ITGAX-1 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immunofluorescence - HPA
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line THP-1 shows localization to plasma membrane & vesicles. Human assay: THP-1 fixed with PFA, dilution: 1:100 Human assay: U2OS fixed with PFA, dilution: 1:100 Click image to enlarge

CPTC-ITGAX-1 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line THP-1 shows localization to plasma membrane & vesicles. Human assay: THP-1 fixed with PFA, dilution: 1:100
Human assay: U2OS fixed with PFA, dilution: 1:100


Characterization SOP Files

  Immunofluorescence - HPA
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line THP-1 shows localization to plasma membrane & vesicles. Human assay: THP-1 fixed with PFA, dilution: 1:100 Human assay: U2OS fixed with PFA, dilution: 1:100 Click image to enlarge

CPTC-ITGAX-1 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line THP-1 shows localization to plasma membrane & vesicles.
Human assay: THP-1 fixed with PFA, dilution: 1:100
Human assay: U2OS fixed with PFA, dilution: 1:100


Characterization SOP Files

  NCI 60 Protein Array

CPTC-ITGAX-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Over-expressed transient protein in cell lysate
Click to enlarge image Western blot using CPTC-ITGAX-1 as primary antibody against human integrin, alpha X (complement component 3 receptor 4 subunit) (ITGAX) transient overexpression lysate (lane 2). Expected molecular weight - 127.8 kDa. Molecular weight standards are also included (lane 1). Target protein is subject to glycosylation which can affect the migration in electrophoresis. This can make the target appear as a higher molecular weight protein. Click image to enlarge

CPTC-ITGAX-1 Western Blot Over-Expressed Lysate

Result: Presumed Positive (with additional bands)

Western blot using CPTC-ITGAX-1 as primary antibody against human integrin, alpha X (complement component 3 receptor 4 subunit) (ITGAX) transient overexpression lysate (lane 2). Expected molecular weight - 127.8 kDa. Molecular weight standards are also included (lane 1). Target protein is subject to glycosylation which can affect the migration in electrophoresis. This can make the target appear as a higher molecular weight protein.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-ITGAX-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 127.8 kDa. Molecular weight standards are also included (lane 1). A549 and NCI-H226 are positive. HeLa and MCF7 are presumed positive. All other cell lines are negative. Target protein is subject to glycosylation which can affect the migration in electrophoresis. This can make the target appear as a higher molecular weight protein. Click image to enlarge

CPTC-ITGAX-1 Automated Western Blot Cell Lysate

Result: Positive

Automated western blot using CPTC-ITGAX-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 127.8 kDa. Molecular weight standards are also included (lane 1). A549 and NCI-H226 are positive.
HeLa and MCF7 are presumed positive. All other cell lines are negative. Target protein is subject to glycosylation which can affect the migration in electrophoresis. This can make the target appear as a higher molecular weight protein.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-ITGAX-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 127.8 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-ITGAX-1 Western Blot Cell Lysate

Result: Negative

Western blot using CPTC-ITGAX-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 127.8 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-ITGAX-1 as primary antibody against human lung (2), spleen (3), endometrium (4), breast (5), and ovary (6) tissue lysates. The expected molecular weight is 127.8 kDa. Vinculin was used as a loading control. Click image to enlarge

CPTC-ITGAX-1 Western Blot (Tissue Lysate)

Result: Negative

Western blot using CPTC-ITGAX-1 as primary antibody against human lung (2), spleen (3), endometrium (4), breast (5), and ovary (6) tissue lysates. The expected molecular weight is 127.8 kDa. Vinculin was used as a loading control.


Characterization SOP Files

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Antigen: Integrin Subunit Alpha X Peptide 1
Background

NCI Identification Number:

00451

Antigen Name:

Integrin Subunit Alpha X Peptide 1

CPTC Name:

CPTC-ITGAX Peptide 1

Aliases:

Integrin Subunit Alpha X; Integrin, Alpha X (Complement Component 3 Receptor 4 Subunit); Integrin, Alpha X (Antigen CD11C (P150), Alpha Polypeptide); Leukocyte Adhesion Glycoprotein P150,95 Alpha Chain; Complement Component 3 Receptor 4 Subunit; Leukocyte Adhesion Receptor P150,95; CD11 Antigen-Like Family Member C; Integrin Alpha-X; CD11C; Leukocyte Surface Antigen P150,95, Alpha Subunit; Myeloid Membrane Antigen, Alpha Subunit; P150 95 Integrin Alpha Chain; Leu M5, Alpha Subunit; Integrin Alpha X; CD11c Antigen; Leu M5; SLEB6; ITGAX; CD11c

Function:

his gene encodes the integrin alpha X chain protein. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles. Two transcript variants encoding different isoforms have been found for this gene.ITGAX (Integrin Subunit Alpha X) is a Protein Coding gene. Diseases associated with ITGAX include Hairy Cell Leukemia and Benign Cephalic Histiocytosis. Among its related pathways are Integrin Pathway and Degradation of the extracellular matrix. An important paralog of this gene is ITGAD.Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis.

Chromosomal Localization:

16p11.2

Accession Number:

NP_001273304.1

UniProt Accession Number:

P20702

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

GVQSLVLGAPR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1210

Last Updated:

09/30/2020

Links

Characterization Data

SOPs

No SOPs available.

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