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Integrin Subunit Alpha 11 Peptide 1

Antibody: CPTC-ITGA11-1
Background

Catalog Number:

CPTC-ITGA11-1

Target Antigen:

Integrin Subunit Alpha 11 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

02/06/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

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Characterization Data [Compare Characterization Data]
  Affinity Measurement by Biolayer Interferometry
Click to enlarge image The affinity and binding kinetics of CPTC-ITGA11-1 and BSA-conjugated peptide “CRREPGLDPTPKVLE” were measured using biolayer interferometry. CPTC-ITGA11-1 antibody was covalently immobilized onto amine-reactive second-generation sensors (AR2G). BSA-conjugated peptide “CRR”, 1 nM, 0.25 nM, 0.0039 nM, and 000977 nM, was used as analyte. Buffer only and biosensors immobilized without antibody were used as references for background subtraction. Click image to enlarge

CPTC-ITGA11-1 Affinity and Kinetics (BLI)

Result: Positive

The affinity and binding kinetics of CPTC-ITGA11-1 and BSA-conjugated peptide “CRREPGLDPTPKVLE” were measured using biolayer interferometry. CPTC-ITGA11-1 antibody was covalently immobilized onto amine-reactive second-generation sensors (AR2G). BSA-conjugated peptide “CRR”, 1 nM, 0.25 nM, 0.0039 nM, and 000977 nM, was used as analyte. Buffer only and biosensors immobilized without antibody were used as references for background subtraction.


Characterization SOP Files

  Affinity Measurement by SPR
Click to enlarge image The affinity and binding kinetics of CPTC-ITGA11-1 and BSA conjugated peptide “CRREPGLDPTPKVLE” were measured using surface plasmon resonance. CPTC-ITGA11-1 antibody was captured onto a Series S Protein A biosensor chip. BSA conjugated peptide, 1.0 nM, 0.25 nM, 0.0625 nM, 0.015625 nM, and 0.0039 nM, was used as analyte. Binding data were double-referenced and analyzed globally. Click image to enlarge

CPTC-ITGA11-1 Affinity and Kinetics (SPR) 

Result: Positive

The affinity and binding kinetics of CPTC-ITGA11-1 and BSA conjugated peptide “CRREPGLDPTPKVLE” were measured using surface plasmon resonance. CPTC-ITGA11-1 antibody was captured onto a Series S Protein A biosensor chip. BSA conjugated peptide, 1.0 nM, 0.25 nM, 0.0625 nM, 0.015625 nM, and 0.0039 nM, was used as analyte. Binding data were double-referenced and analyzed globally.


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Micro-Array (TMA) core of prostate cancer showing cytoplasmic and membranous staining using Antibody CPTC-ITGA11-1. Titer: 1:1000 Click image to enlarge

CPTC-ITGA11-1 IHC Tissue

Result: Positive

Tissue Micro-Array (TMA) core of prostate cancer showing cytoplasmic and membranous staining using Antibody CPTC-ITGA11-1. Titer: 1:1000


Characterization SOP Files

  IP
Click to enlarge image Immuno-precipitation performed using CPTC-ITGA11-1 as capture antibody against human mesenchymal stem cell (hMSC). Eluate was tested in automated western blot using the same CPTC_ITGA11-1 as detection antibody. The target protein was detected only in the whole lysate (lane hMSC – SM), and not in the IP eluate (lane hMSC – IP). Click image to enlarge

CPTC-ITGA11-1 – IP-Wes

Result: Negative

Immuno-precipitation performed using CPTC-ITGA11-1 as capture antibody against human mesenchymal stem cell (hMSC). Eluate was tested in automated western blot using the same CPTC_ITGA11-1 as detection antibody. The target protein was detected only in the whole lysate (lane hMSC – SM), and not in the IP eluate (lane hMSC – IP).


Characterization SOP Files

  Immunofluorescence
Click to enlarge image Immunofluorescence staining using CPTC-ITGA11-1 as primary antibody against human mesenchymal stem cells. CPTC-ITGA11-1 ab shows localization in the cytoplasm (green). Click image to enlarge

CPTC-ITGA11-1 Immunofluorescence

Result: Positive

Immunofluorescence staining using CPTC-ITGA11-1 as primary antibody against human mesenchymal stem cells. CPTC-ITGA11-1 ab shows localization in the cytoplasm (green).


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-ITGA11-1 as primary antibody against BSA-conjugated peptide “CRREPGLDPTPKVLE”. Click image to enlarge

CPTC-ITGA11-1 Indirect ELISA 

Result: Positive

Indirect ELISA using CPTC-ITGA11-1 as primary antibody against BSA-conjugated peptide “CRREPGLDPTPKVLE”.


Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-ITGA11-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-ITGA11-1 NCI 60 Protein Array

Result: Positive

Protein Array in which CPTC-ITGA11-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Automated Western Blot using CPTC-ITGA11-1 as primary antibody against human and mouse recombinant proteins. The antibody CPTC-ITGA11-1 specifically recognizes the human recombinant. Expected MW is 133 KDa, but ITGA11 glycosylation affects its migration. Click image to enlarge

CPTC-ITGA11-1 Automated western blot (recombinant protein)

Result: Positive

Automated Western Blot using CPTC-ITGA11-1 as primary antibody against human and mouse recombinant proteins. The antibody CPTC-ITGA11-1 specifically recognizes the human recombinant. Expected MW is 133 KDa, but ITGA11 glycosylation affects its migration.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-ITGA11-1 as primary antibody against human and mouse recombinant proteins. The antibody CPTC-ITGA11-1 specifically recognizes the human recombinant. Expected MW is 133 KDa, but ITGA11 glycosylation affects its migration. Click image to enlarge

CPTC-ITGA11-1 western blot (recombinant protein)

Result: Positive

Western Blot using CPTC-ITGA11-1 as primary antibody against human and mouse recombinant proteins. The antibody CPTC-ITGA11-1 specifically recognizes the human recombinant. Expected MW is 133 KDa, but ITGA11 glycosylation affects its migration.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated Western Blot using CPTC-ITGA11-1 as primary antibody against cell lysate of human mesenchymal stem cells (hMSC), A549, HeLa and MCF7. The antibody CPTC-ITGA11-1 recognizes the target protein in hMSC and HeLa lysates. Expected MW is 133 KDa, but ITGA11 glycosylation affects its migration. Click image to enlarge

CPTC-ITGA11-1 Automated western blot (lysates)

Result: Positive

Automated Western Blot using CPTC-ITGA11-1 as primary antibody against cell lysate of human mesenchymal stem cells (hMSC), A549, HeLa and MCF7. The antibody CPTC-ITGA11-1 recognizes the target protein in hMSC and HeLa lysates. Expected MW is 133 KDa, but ITGA11 glycosylation affects its migration.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-ITGA11-1 as primary antibody against cell lysate of human mesenchymal stem cells (hMSC), A549, HeLa and MCF7. The antibody CPTC-ITGA11-1 presumably recognizes the target protein in hMSC A549, HeLa and MCF7 lysates. Expected MW is 133 KDa, but ITGA11 glycosylation affects its migration. Click image to enlarge

CPTC-ITGA11-1 western blot (lysates)

Result: Presumed Positive (with additional bands)

Western Blot using CPTC-ITGA11-1 as primary antibody against cell lysate of human mesenchymal stem cells (hMSC), A549, HeLa and MCF7. The antibody CPTC-ITGA11-1 presumably recognizes the target protein in hMSC A549, HeLa and MCF7 lysates. Expected MW is 133 KDa, but ITGA11 glycosylation affects its migration.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Single cell western blot using CPTC-ITGA11-1 as a primary antibody against human mesenchymal stem cell lysates. ITGA11 protein expression was detected. Click image to enlarge

CPTC-ITGA11-1 Single Cell Western Blot (Cell Lysate)

Result: Positive

Single cell western blot using CPTC-ITGA11-1 as a primary antibody against human mesenchymal stem cell lysates. ITGA11 protein expression was detected.


Characterization SOP Files

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Antigen: Integrin Subunit Alpha 11 Peptide 1
Background

NCI Identification Number:

00503

Antigen Name:

Integrin Subunit Alpha 11 Peptide 1

CPTC Name:

CPTC-ITGA11 Peptide 1

Aliases:

Integrin Subunit Alpha 11; HsT18964; Integrin Alpha-11; Integrin, Alpha 11

Function:

This gene encodes an alpha integrin. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein contains an I domain, is expressed in muscle tissue, dimerizes with beta 1 integrin in vitro, and appears to bind collagen in this form. Therefore, the protein may be involved in attaching muscle tissue to the extracellular matrix. Alternative transcriptional splice variants have been found for this gene, but their biological validity is not determined.ITGA11 (Integrin Subunit Alpha 11) is a Protein Coding gene. Diseases associated with ITGA11 include Brain Oligodendroglioma and Lung Cancer. Among its related pathways are MAPK Pathway and Degradation of the extracellular matrix. Gene Ontology (GO) annotations related to this gene include collagen binding and collagen receptor activity. An important paralog of this gene is ITGA10.

Chromosomal Localization:

15q23

Accession Number:

NP_001004439.1

UniProt Accession Number:

Q9UKX5

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

CRREPGLDPTPKVLE

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1650

Last Updated:

10/21/2021

Links

Characterization Data

SOPs

No SOPs available.

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