Background
Catalog Number:
CPTC-IL2RG-1
Target Antigen:
Interleukin 2 Receptor Subunit Gamma Peptide 1
Isotype:
IgG
Species:
Rabbit Monoclonal Antibody
Last Updated:
02/06/2024
Antigen Recognition(s):
Peptide, Endogenous
Purchase
- DSHB CPTC-IL2RG-1
External Links
- Human Protein Atlas CPTC-IL2RG-1
Characterization Data [Compare Characterization Data]
IHC HPA
Download This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org). (542.5 KB)
CPTC-IL2RG-1 evaluation by the Human Protein Atlas
Result: Positive
This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
IHC Tissue
CPTC-IL2RG-1 IHC Tissue
Result: Presumed Positive
Tissue Micro-Array (TMA) core of breast cancer showing cytoplasmic staining using Antibody CPTC-IL2RG-1. Titer: 1:50
The pattern of staining is correct but lacks sufficient literature data to confirm cell type specificity.
Characterization SOP Files
Immunofluorescence
CPTC-IL2RG-1 Immunofluorescence
Result: Negative
Immunofluorescence staining of HeLa cells using CPTC-IL2RG-1 antibody. IL2RG protein expression was not detected.
Characterization SOP Files
Immunofluorescence - HPA
CPTC-IL2RG-1 evaluation by the Human Protein Atlas
Result: Positive
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Immunofluorescent staining of human cell line THP-1 shows localization to nucleoplasm & plasma membrane. Human assay: THP-1 fixed with PFA, dilution: 1:150
Characterization SOP Files
NCI 60 Protein Array
CPTC-IL2RG-1 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-IL2RG-1 Western Blot Recombinant Protein
Result: Positive
Western blot using CPTC-IL2RG-1 as primary antibody against human CD132 / IL2RG (23-262, His-tag) recombinant protein (lane 2). Expected molecular weight - 29.0 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-IL2RG-1 Automated Western Blot Cell Lysate
Result: Negative
Automated western blot using CPTC-IL2RG-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 42.3 kDa and 20.1 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-IL2RG-1 Western Blot Cell Lysate
Result: Presumed Positive (with additional bands)
Western blot using CPTC-IL2RG-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 42.3 kDa and 20.1 kDa. Molecular weight standards are also included (lane 1). MCF7 and NCI-H226 are presumed positive. All the other cell lines are negative.
Characterization SOP Files
Background
NCI Identification Number:
00449
Antigen Name:
Interleukin 2 Receptor Subunit Gamma Peptide 1
CPTC Name:
CPTC-IL2RG Peptide 1
Aliases:
Interleukin 2 Receptor Subunit Gamma; Cytokine Receptor Common Subunit Gamma; Interleukin 2 Receptor, Gamma; IL-2 Receptor Subunit Gamma; IL-2R Subunit Gamma; CD132 Antigen; IL-2RG; GammaC; CD132; P64; Nonfunctional Common Cytokine Receptor Gamma Chain; Common Cytokine Receptor Gamma Chain; Interleukin-2 Receptor Subunit Gamma; Combined Immunodeficiency, X-Linked; Severe Combined Immunodeficiency; SCIDX1; SCIDX; IL2RG; CIDX; IMD4
Function:
The protein encoded by this gene is an important signaling component of many interleukin receptors, including those of interleukin -2, -4, -7 and -21, and is thus referred to as the common gamma chain. Mutations in this gene cause X-linked severe combined immunodeficiency (XSCID), as well as X-linked combined immunodeficiency (XCID), a less severe immunodeficiency disorder.IL2RG (Interleukin 2 Receptor Subunit Gamma) is a Protein Coding gene. Diseases associated with IL2RG include Severe Combined Immunodeficiency, X-Linked and Combined Immunodeficiency, X-Linked. Among its related pathways are Common Cytokine Receptor Gamma-Chain Family Signaling Pathways and RET signaling. Gene Ontology (GO) annotations related to this gene include cytokine receptor activity and interleukin-2 binding. An important paralog of this gene is ENSG00000285171Common subunit for the receptors for a variety of interleukins. Probably in association with IL15RA, involved in the stimulation of neutrophil phagocytosis by IL15
Chromosomal Localization:
Xq13.1
Accession Number:
NP_000197.1
UniProt Accession Number:
P31785
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
GLAESLQPDYSER
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
1430
Last Updated:
09/30/2020
Links
- EnsEMBL - EnsEMBL IL2RG
- Entrez - Entrez IL2RG
- Uniprot - Uniprot P31785
Characterization Data
SOPs
No SOPs available.
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