Background
Catalog Number:
CPTC-IL1RN-1
RRID:
AB_2890088
Target Antigen:
Interleukin 1 Receptor Antagonist Peptide 1
Isotype:
IgG
Species:
Rabbit Monoclonal Antibody
Last Updated:
07/03/2024
Antigen Recognition(s):
Peptide, Recombinant Full-length, Endogenous
External Links
- CPTAC Assay Portal CPTC-IL1RN-1
Purchase
- DSHB CPTC-IL1RN-1
External Links
- Human Protein Atlas CPTC-IL1RN-1
Characterization Data [Compare Characterization Data]
IHC HPA
Download This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org). (502.6 KB)
CPTC-IL1RN-1 evaluation by the Human Protein Atlas
Result: Positive
This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
Immuno-MRM
CPTC-IL1RN-1 iMRM (Frozen Tissue)
Result: Positive
Immuno-MRM chromatogram of CPTC-IL1RN-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6206)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue
Characterization SOP Files
Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (502.6 KB)
CPTC-IL1RN-1 evaluation by the Human Protein Atlas
Result: Negative
Results provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
NCI 60 Protein Array
CTPC-IL1RN-1 NCI60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - HPA - tissue or cell lysate
CPTC-IL1RN-1 evaluation by the Human Protein Atlas
Result: Positive
Single band corresponding to the predicted size in kDa (+/-20%).
Analysis performed using a standard panel of samples. Antibody dilution: 1:500
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-IL1RN-1 Western Blot Recombinant Protein
Result: Presumed Positive (with additional bands)
Western blot using CPTC-IL1RN-1 as primary antibody against human interleukin 1 receptor antagonist (IL1RN), transcript variant 1 recombinant protein (lane 2). Expected molecular weight - 17.1 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-IL1RN-1 Western Blot Cell Lysate
Result: Negative
Western blot using CPTC-IL1RN-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and NCI-H226 (lane 6) whole cell lysates. Expected molecular weight - 20.1 kDa, 17.9 kDa, 19.9 kDa, and 16.1 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-IL1RN-1 Automated Western Blot Cell Lysate
Result: Negative
Automated western blot using CPTC-IL1RN-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 20.1 kDa, 17.9 kDa, 19.9 kDa, and 16.1 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Background
NCI Identification Number:
00409
Antigen Name:
Interleukin 1 Receptor Antagonist Peptide 1
CPTC Name:
CPTC-IL1RN Peptide 1
Aliases:
Interleukin 1 Receptor Antagonist; Interleukin-1 Receptor Antagonist Protein; ICIL-1RA; IL-1RN; IL1F3; IL1RA; IRAP; IL1 Inhibitor; IL-1ra; Intracellular Interleukin-1 Receptor Antagonist (IcIL-1ra); Intracellular Interleukin-1 Receptor Antagonist; Intracellular IL-1 Receptor Antagonist Type II; Type II Interleukin-1 Receptor Antagonist; MGC10430; Anakinra; IL-1ra3; MVCD4; IL1RN; DIRA
Function:
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Several alternatively spliced transcript variants encoding distinct isoforms have been reported.IL1RN (Interleukin 1 Receptor Antagonist) is a Protein Coding gene. Diseases associated with IL1RN include Osteomyelitis, Sterile Multifocal, With Periostitis And Pustulosis and Microvascular Complications Of Diabetes 4. Among its related pathways are PEDF Induced Signaling and Prolactin Signaling Pathway. Gene Ontology (GO) annotations related to this gene include cytokine activity and interleukin-1 receptor antagonist activity. An important paralog of this gene is IL36RN.Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.
Chromosomal Localization:
2q14.1
Accession Number:
NP_776214.1
UniProt Accession Number:
P18510
DNA Source:
N/A
Immunogen:
Recombinant Full Length Protein
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
IDVVPIEPHALFLGIHGGK
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
2090
Last Updated:
09/03/2020
Links
- EnsEMBL - EnsEMBL IL1RN
- Entrez - Entrez IL1RN
- Uniprot - Uniprot P18510
Characterization Data
SOPs
No SOPs available.
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