Catalog Number:
CPTC-IDO1-4
Target Antigen:
Indoleamine 2,3-Dioxygenase 1 Peptide 2
Isotype:
IgG
Species:
Rabbit Monoclonal Antibody
Last Updated:
04/20/2023
Antigen Recognition(s):
Peptide, Recombinant Full-length
Result: Positive
The affinity and binding kinetics of CPTC-IDO1-4 antibody and BSA-conjugated peptide “NIAVPYCQLSK" were measured using biolayer interferometry. BSA-conjugated peptide was covalently immobilized onto AR2G biosensors using standard amine coupling. CPTC-IDO1-4 antibody, 1024 nM, 256 nM, 64 nM, 4 nM, and 0.25 nM, was used as analyte. Buffer only and biosensors immobilized without BSA-conjugated peptide were used as references for background subtraction. All data was analyzed globally using a 1:2 bivalent model.
Result: Positive
The affinity and binding kinetics of CPTC-IDO1-4 antibody and BSA-conjugated peptide, “NIAVPYCQLSK”, were measured using surface plasmon resonance. Peptide was amine coupled onto a Series S CM5 biosensor chip. Antibody at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1.0 nM and 0.25 nM, was used as analyte. Binding data were double-referenced and analyzed globally using a bivalent fitting model.
Result: Positive
Immuno-MRM using CPTC-IDO1-4 as capture antibody against the synthetic peptide NIAVPYCQLSK. Antibody CPTC-IDO1-4 captures the synthetic peptide.
Result: Negative
Immunofluorescence staining using CPTC-IDO1-4 as primary antibody against MCF7 cells shows no localization of IDO1 protein.
Result: Positive
Indirect ELISA using CPTC-IDO1-4 as primary antibody against BSA conjugated peptide, "NIAVPYCQLSK", coated on the plate and detected using HRP conjugated goat anti-rabbit secondary antibody and TMB.
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Result: Positive
Western blot using CPTC-IDO1-4 as primary antibody against human indoleamine 2,3-dioxygenase 1 (IDO1) recombinant protein. The expected molecular weight is 45.1 kDa.
Result: Presumed Positive (with additional bands)
Automated western blot using CPTC-IDO1-4 as primary antibody against recombinant IDO1 protein. Protein molecular weight is about 45 KDa. The antibody presumably recognizes recombinant IDO1.
Result: Presumed Positive (with additional bands)
Western blot using CPTC-IDO1-4 as primary antibody against whole cell lysates CCRF-CEM (lane 2), HeLa (lane 3), Jurkat (lane 4), K-562 (lane 5), and MCF7 (lane 6). The expected molecular weight is 45.3 kDa. MCF7 is presumed positive. All other cell lines are negative.
Result: Negative
Automated western blot using CPTC-IDO1-4 as primary antibody against whole cell lysates of cell lines CCRF-CEM, HeLa, Jurkat, K-562 and MCF7. Protein molecular weight is about 45 KDa. The antibody cannot recognize IDO1 in the tested lysates. Loading controls were run with anti-GAPDH antibody.
Result: Negative
Single cell western blot using CPTC-IDO1-4 as a primary antibody against MCF7 cell lysate. IDO1 protein expression was not detected.
NCI Identification Number:
00522
Antigen Name:
Indoleamine 2,3-Dioxygenase 1 Peptide 2
CPTC Name:
CPTC-IDO1 Peptide 2
Aliases:
Indoleamine 2,3-Dioxygenase 1; INDO; IDO; Indoleamine-Pyrrole 2,3-Dioxygenase; IDO-1; Indoleamine-Pyrrole 2,3 Dioxygenase; Indolamine 2,3 Dioxygenase; Indole 2,3-Dioxygenase; EC 1.13.11.52
Function:
This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.IDO1 (Indoleamine 2,3-Dioxygenase 1) is a Protein Coding gene. Diseases associated with IDO1 include Listeriosis and Trypanosomiasis. Among its related pathways are Histidine, lysine, phenylalanine, tyrosine, proline and tryptophan catabolism and superpathway of tryptophan utilization. Gene Ontology (GO) annotations related to this gene include heme binding and tryptophan 2,3-dioxygenase activity.Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway (PubMed:17671174). Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses (PubMed:25691885). Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells (PubMed:25691885). Acts as a suppressor of anti-tumor immunity (PubMed:23103127, PubMed:25157255, PubMed:14502282, PubMed:25691885). Limits the growth of intracellular pathogens by depriving tryptophan (PubMed:25691885). Protects the fetus from maternal immune rejection (PubMed:25691885).
Chromosomal Localization:
8p11.21
Accession Number:
NP_002155.1
UniProt Accession Number:
P14902
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
NIAVPYCQLSK
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
1210
Last Updated:
06/09/2022
No SOPs available.
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