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Fascin 1, Actin-Bundling Protein

Antibody: CPTC-Fascin1-1
Background

Catalog Number:

CPTC-Fascin1-1

RRID:

AB_3101835

Target Antigen:

Fascin 1, Actin-Bundling Protein

Isotype:

IgG2c

Species:

Mouse Monoclonal Antibody

Last Updated:

06/11/2024

Antigen Recognition(s):

Recombinant Full-length, Endogenous

SOP:

Purchase
External Links
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (180.5 KB)

CPTC-Fascin-1 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immunofluorescence - HPA
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol. Human assay: A-431 fixed with PFA, dilution: 1:500 Human assay: U-2 OS fixed with PFA, dilution: 1:500 Human assay: U-251 MG fixed with PFA, dilution: 1:500 Click image to enlarge

CPTC-Fascin-1 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol. Human assay: A-431 fixed with PFA, dilution: 1:500
Human assay: U-2 OS fixed with PFA, dilution: 1:500
Human assay: U-251 MG fixed with PFA, dilution: 1:500


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

CPTC-Fascin 1-1 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)


Characterization SOP Files

  Western Blot - HPA - tissue or cell lysate
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). Band of predicted size in kDa (+/-20%) with additional bands present. Analysis performed using a standard panel of samples. Antibody dilution: 1:500 Click image to enlarge

CPTC-Fascin-1 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org). Band of predicted size in kDa (+/-20%) with additional bands present. Analysis performed using a standard panel of samples. Antibody dilution: 1:500


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-Fascin 1-1 as primary Ab against Ag 10411 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-Fascin 1-1 Western Blot

Result: Positive

Western Blot using CPTC-Fascin 1-1 as primary Ab against Ag 10411 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antibody: CPTC-Fascin1-2
Background

Catalog Number:

CPTC-Fascin1-2

RRID:

AB_2106397

Target Antigen:

Fascin 1, Actin-Bundling Protein

Isotype:

IgG2c

Species:

Mouse Monoclonal Antibody

Last Updated:

09/09/2021

Antigen Recognition(s):

Recombinant Full-length, Endogenous

SOP:

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (281.7 KB)

CPTC-Fascin 1-2 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  IHC NCI60

CPTC-Fascin 1-2 IHC NCI60

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  IHC Tissue

CPTC-Fascin 1-2 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

CPTC-Fascin 1-2 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)


Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-Fascin 1-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-Fascin 1-2 Protein Array

Result: Positive

Protein Array in which CPTC-Fascin 1-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-Fascin 1-2 as primary Ab against Ag 10411 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-Fascin 1-2 Western Blot

Result: Positive

Western Blot using CPTC-Fascin 1-2 as primary Ab against Ag 10411 (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antibody: CPTC-Fascin1-3
Background

Catalog Number:

CPTC-Fascin1-3

RRID:

AB_2617259

Target Antigen:

Fascin 1, Actin-Bundling Protein

Isotype:

IgG2b

Species:

Mouse Monoclonal Antibody

Last Updated:

09/09/2021

Antigen Recognition(s):

Recombinant Full-length, Endogenous

SOP:

Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (281.7 KB)

CPTC-Fascin 1-3 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Antigen coated plate) Click image to enlarge

CPTC-Fascin 1-3 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to Antigen coated plate)


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-Fascin-3 as primary Ab against CPTC-Fascin (Ag 10411) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3). Click image to enlarge

CPTC-Fascin 1-3 Western Blot

Result: Positive

Western Blot using CPTC-Fascin-3 as primary Ab against CPTC-Fascin (Ag 10411) (lane 2). Also included are molecular wt. standards (lane 1) and mouse IgG control (lane 3).


Characterization SOP Files

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Antigen: Fascin 1, Actin-Bundling Protein
Background

NCI Identification Number:

10411

Antigen Name:

Fascin 1, Actin-Bundling Protein

CPTC Name:

CPTC-Fascin 1

Aliases:

FSCN1; FAN1; FLJ38511; Fascin; HSN; SNL; p55

Function:

Chromosomal Localization:

7p22

Expression System:

E. Coli

Accession Number:

BC007948

UniProt Accession Number:

Q16658

DNA Source:

HIP:HsCD00004052

Immunogen:

Recombinant Full Length Protein

Vector Name:

pMCSG7

Extinction Coefficient:

68153

Buffers:

50mM NH4HC03

Expressed Sequence:

SNAMTANGTAEAVQIQFGLINCGNKYLTAEAFGFKVNASASSLKKKQIWT
LEQPPDEAGSAAVCLRSHLGRYLAADKDGNVTCEREVPGPDCRFLIVAHD
DGRWSLQSEAHRRYFGGTEDRLSCFAQTVSPAEKWSVHIAMHPQVNIYSV
TRKRYAHLSARPADEIAVDRDVPWGVDSLITLAFQDQRYSVQTADHRFLR
HDGRLVARPEPATGYTLEFRSGKVAFRDCEGRYLAPSGPSGTLKAGKATK
VGKDELFALEQSCAQVVLQAANERNVSTRQGMDLSANQDEETDQETFQLE
IDRDTKKCAFRTHTGKYWTLTATGGVQSTASSKNASCYFDIEWRDRRITL
RASNGKFVTSKKNGQLAASVETAGDSELFLMKLINRPIIVFRGEHGFIGC
RKVTGTLDANRSSYDVFQLEFNDGAYNIKDSTGKYWTVGSDSAVTSSGDT
PVDFFFEFCDYNKVAIKVGGRYLKGDHAGVLKASAETVDPASLWEY

Native Sequence:

MTANGTAEAVQIQFGLINCGNKYLTAEAFGFKVNASASSLKKKQIWTLEQ
PPDEAGSAAVCLRSHLGRYLAADKDGNVTCEREVPGPDCRFLIVAHDDGR
WSLQSEAHRRYFGGTEDRLSCFAQTVSPAEKWSVHIAMHPQVNIYSVTRK
RYAHLSARPADEIAVDRDVPWGVDSLITLAFQDQRYSVQTADHRFLRHDG
RLVARPEPATGYTLEFRSGKVAFRDCEGRYLAPSGPSGTLKAGKATKVGK
DELFALEQSCAQVVLQAANERNVSTRQGMDLSANQDEETDQETFQLEIDR
DTKKCAFRTHTGKYWTLTATGGVQSTASSKNASCYFDIEWRDRRITLRAS
NGKFVTSKKNGQLAASVETAGDSELFLMKLINRPIIVFRGEHGFIGCRKV
TGTLDANRSSYDVFQLEFNDGAYNIKDSTGKYWTVGSDSAVTSSGDTPVD
FFFEFCDYNKVAIKVGGRYLKGDHAGVLKASAETVDPASLWEY

Calculated Isoelectric Point:

6.83

Molecular Weight:

54802

Last Updated:

07/18/2019

Links

Characterization Data

Gel

Click to enlarge image PAGE of Fascin (rAg 10411) with molecular weight standards in lane 1.
Click image to enlarge

CPTC-Fascin 1 (rAg 10411) PAGE

PAGE of Fascin (rAg 10411) with molecular weight standards in lane 1.

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SOPs

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