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Forkhead Box Q1 Peptide 1

Antibody: CPTC-FOXQ1-1
Background

Catalog Number:

CPTC-FOXQ1-1

RRID:

AB_2827870

Target Antigen:

Forkhead Box Q1 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

02/06/2024

Antigen Recognition(s):

Recombinant Full-length

Thematic Panel(s):

Epithelial Mesenchymal Transition (EMT)

Purchase
Publications
Characterization Data [Compare Characterization Data]
  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (573.1 KB)

CPTC-FOXQ1-1 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  IP
Download Immuno-precipitation data obtained incubating CPTC-FOXQ1-1 and the recombinant domain of FOXQ1 (aa1-110, expected, MW ~14KDa) (170.6 KB)

CPTC-FOXQ1-1 IPMS

Result: Negative

Immuno-precipitation data obtained incubating CPTC-FOXQ1-1 and the recombinant domain of FOXQ1 (aa1-110, expected, MW ~14KDa)


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (573.1 KB)

CPTC-FOXQ1-1 evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide

CPTC-FOXQ1-1 recombinant protein WB

Result: Negative

Western Blot using CPTC-FOXQ1-1 as primary antibody against FOXQ1 protein domain comprising amino acids 1-110 with expected MW of 10.1 KDa.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-FOXQ1-1 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 41.5 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-FOXQ1-1 Endogenous Western Blot

Result: Negative

Western Blot using CPTC-FOXQ1-1 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 41.5 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated Western Blot using CPTC-FOXQ1-1 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 41.5 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-FOXQ1-1 Endogenous Automated Western Blot

Result: Negative

Automated Western Blot using CPTC-FOXQ1-1 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 41.5 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1).


Characterization SOP Files

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Antibody: CPTC-FOXQ1-2
Background

Catalog Number:

CPTC-FOXQ1-2

RRID:

AB_2827871

Target Antigen:

Forkhead Box Q1 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

02/15/2024

Antigen Recognition(s):

Recombinant Full-length, Endogenous

Thematic Panel(s):

Epithelial Mesenchymal Transition (EMT)

External Links
Purchase
External Links
Publications
Characterization Data [Compare Characterization Data]
  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (623.5 KB)

CPTC-FOXQ1-2 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  IP
Download Immuno-precipitation data obtained incubating CPTC-FOXQ1-2 and the recombinant domain of FOXQ1 (aa1-110, expected, MW ~14KDa) (170.8 KB)

CPTC_FOXQ1-2 IPMS

Result: Negative

Immuno-precipitation data obtained incubating CPTC-FOXQ1-2 and the recombinant domain of FOXQ1 (aa1-110, expected, MW ~14KDa)


Characterization SOP Files

  Immunofluorescence

CPTC-FOXQ1-2 Immunofluorescence

Result: Presumed Positive

Immunofluorescence staining of human cell line U-2 OS using CPTC-FOXQ1-2 Ab as primary antibody. FOXQ1 protein expression shows weak localization to the nucleoplasm.


Characterization SOP Files

  Immunofluorescence - HPA
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is partly supported by literature or no literature is available. Immunofluorescent staining of human cell line U2OS shows localization to nucleoplasm. Human assay: THP-1 fixed with PFA, dilution: 1:125 Human assay: U2OS fixed with PFA, dilution: 1:125 Click image to enlarge

CPTC-FOXQ1-2 evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is partly supported by literature or no literature is available. Immunofluorescent staining of human cell line U2OS shows localization to nucleoplasm.


Human assay: THP-1 fixed with PFA, dilution: 1:125
Human assay: U2OS fixed with PFA, dilution: 1:125


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide

CPTC-FOXQ1-2 recombinant protein WB

Result: Negative

Western Blot using CPTC-FOXQ1-1 as primary antibody against FOXQ1 protein domain comprising amino acids 1-110 with expected MW of 10.1 KDa.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated Western Blot using CPTC-FOXQ1-2 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 41.5 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-FOXQ1-2 Endogenous Automated Western Blot

Result: Negative

Automated Western Blot using CPTC-FOXQ1-2 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 41.5 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-FOXQ1-2 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 41.5 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-FOXQ1-2 Endogenous Western Blot

Result: Negative

Western Blot using CPTC-FOXQ1-2 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 41.5 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1).


Characterization SOP Files

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Antigen: Forkhead Box Q1 Peptide 1
Background

NCI Identification Number:

00267

Antigen Name:

Forkhead Box Q1 Peptide 1

CPTC Name:

CPTC-FOXQ1 Peptide 1

Aliases:

Forkhead Box Q1; Hepatocyte Nuclear Factor 3 Forkhead Homolog 1; HNF-3/Forkhead-Like Protein 1; HFH-1; HFH1; Winged Helix/Forkhead Transcription Factor; Forkhead Box Protein Q1

Function:

FOXQ1 is a member of the FOX gene family, which is characterized by a conserved 110-amino acid DNA-binding motif called the forkhead or winged helix domain. FOX genes are involved in embryonic development, cell cycle regulation, tissue-specific gene expression, cell signaling, and tumorigenesis (Bieller et al., 2001 [PubMed 11747606]).

FOXQ1 (Forkhead Box Q1) is a Protein Coding gene. Diseases associated with FOXQ1 include Ritscher-Schinzel Syndrome. Among its related pathways are Preimplantation Embryo. Gene Ontology (GO) annotations related to this gene include DNA binding transcription factor activity and RNA polymerase II transcription factor activity, sequence-specific DNA binding. An important paralog of this gene is FOXD2.

Plays a role in hair follicle differentiation.

Chromosomal Localization:

6p25.3

Accession Number:

NP_150285.3

UniProt Accession Number:

Q9C009

DNA Source:

The NCI acknowledges the generous advice and gene clones provided by Robert A. Weinberg at the Ludwig Center for Cancer Research of the Massachusetts Institute of Technology. These reagents have been provided by the Division of Cancer Treatment and Diagnosis (DCTD) of the National Cancer Institute (NCI) using funding of the American Recovery and Reinvestment Act of 2009

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

MKLEVFVPRAAHGDKQGSDLEGc

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

2420

Last Updated:

09/16/2019

Links

Characterization Data

SOPs

No SOPs available.

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