Background
Catalog Number:
CPTC-FOS-1
RRID:
AB_2722058
Target Antigen:
FBJ Murine Osteosarcoma Viral Oncogene Homolog Peptide 3
Isotype:
IgG2b
Species:
Mouse Monoclonal Antibody
Last Updated:
05/23/2024
Antigen Recognition(s):
Peptide, Recombinant Full-length, Endogenous
Thematic Panel(s):
Ras Pathway
Purchase
- DSHB CPTC-FOS-1
Publications
Characterization Data [Compare Characterization Data]
CyTOF
CPTC-FOS-1 Immuno-Mass Cytometry
Result: Positive
Imaging mass cytometry on normal liver tissue core using CPTC-FOS-1 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (liver, bone marrow, spleen, placenta, prostate, colon, pancreas, breast, lung, testis, endometrium, appendix, and kidney) and cancer tissues (colon, breast, ovarian, lung, and prostate).
IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (323.7 KB)
CPTC-FOS-1 Evaluation by the Human Protein Atlas
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
IHC Tissue
CPTC-FOS-1
Result: Positive
Tissue Microarray core of lung cancer immunohistochemically stained with antibody CPTC-FOS-1
Characterization SOP Files
Immuno-MRM
CPTC-FOS-1 iMRM
Result: Positive
Immuno-MRM chromatogram of CPTC-FOS-1 antibody with CPTC-FOS peptide 3 (NCI ID#00142) as target
Immunofluorescence
CPTC-FOS-1 Immunofluorescence
Result: Negative
Immunofluorescence staining of MCF10a cells stably transfected with KRAS (MCF10a-KRAS) using CPTC-FOS-1 antibody. FOS protein expression was not detected.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-FOS-1 Western Blot
Result: Positive
Western Blot using CPTC-FOS-1 as primary Ab against recombinant FOS (lane 2). Also included are molecular wt. standards (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-FOS-1 Cell Lysate Western Blot
Result: Positive
Western Blot using CPTC-FOS-1 as primary Ab against MCF10A-KRAS cell lysate (lane 2). Also included are molecular wt. standards (lane 1).
Characterization SOP Files
Background
Catalog Number:
CPTC-FOS-2
RRID:
AB_2722059
Target Antigen:
FBJ Murine Osteosarcoma Viral Oncogene Homolog Peptide 3
Isotype:
IgG1
Species:
Mouse Monoclonal Antibody
Last Updated:
08/23/2022
Antigen Recognition(s):
Peptide
Thematic Panel(s):
Ras Pathway
Purchase
- DSHB CPTC-FOS-2
Publications
- Integrated Proteogenomic Characterization across Major Histological Types of Pediatric Brain Cancer (PMID: 33242424)
- Targeted mass-spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP kinase, and AKT signaling (PMID: 34671754)
Characterization Data [Compare Characterization Data]
IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (354.7 KB)
CPTC-FOS-2 Evaluation by the Human Protein Atlas
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
Immuno-MRM
CPTC-FOS-2 iMRM
Result: Positive
Immuno-MRM chromatogram of CPTC-FOS-1 antibody with CPTC-FOS peptide 3 (NCI ID#00142) as target
Background
NCI Identification Number:
00142
Antigen Name:
FBJ Murine Osteosarcoma Viral Oncogene Homolog Peptide 3
CPTC Name:
CPTC-FOS Peptide 3
Aliases:
FBJ Murine Osteosarcoma Viral Oncogene Homolog; G0/G1 Switch Regulatory Protein 7; FBJ Murine Osteosarcoma Viral (V-Fos) Oncogene Homolog (Oncogene FOS); V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog; Cellular Oncogene C-Fos; Cellular Oncogene Fos; Activator Protein 1; C-FOS; AP-1; G0S7; P55
Function:
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death.
FOS (FBJ Murine Osteosarcoma Viral Oncogene Homolog) is a Protein Coding gene. Diseases associated with FOS include myositis ossificans and cerebral hypoxia. Among its related pathways are Gene Expression and IL6-mediated signaling events. GO annotations related to this gene include transcription factor activity, sequence-specific DNA binding and transcription factor binding. An important paralog of this gene is FOSL1.
Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.
Chromosomal Localization:
14q24.3
Accession Number:
NP_005243.1
UniProt Accession Number:
P01100
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
TEPFDDFLFPASSR
Native Sequence:
Calculated Isoelectric Point:
0
Molecular Weight:
1540
Last Updated:
07/12/2016
Links
- Entrez - Entrez FOS
- EnsEMBL - EnsEMBL FOS
- Uniprot - Uniprot P01100
Characterization Data
SOPs
No SOPs available.
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