Background
Catalog Number:
CPTC-FAS-1
RRID:
AB_2889962
Target Antigen:
Fas Cell Surface Death Receptor Peptide 1
Isotype:
IgG
Species:
Rabbit Monoclonal Antibody
Last Updated:
03/20/2024
Antigen Recognition(s):
Peptide, Endogenous
Thematic Panel(s):
Immuno-Oncology (IO)
External Links
- CPTAC Assay Portal CPTAC-5953
- CPTAC Assay Portal CPTAC-5954 (Phospho)
Purchase
- DSHB CPTC-FAS-1
Publications
Characterization Data [Compare Characterization Data]
IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (430.9 KB)
CPTC-FAS-1 Evaluation by the Human Protein Atlas
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
Immuno-MRM
CPTC-FAS-1 iMRM
Result: Positive
Immuno-MRM chromatogram of CPTC-FAS-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-5953 for non-phosphorylated peptide and https://assays.cancer.gov/CPTAC-5954 for phosphorylated peptide)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from FFPE tumor tissue lysate pool.
Characterization SOP Files
NCI 60 Protein Array
CTPC-FAS-1 NCI60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-FAS-1 Western Blot (recombinant protein)
Result: Negative
Western blot using CPTC-FAS-1 as primary antibody against human Fas (TNF receptor superfamily, member 6) (FAS), transcript variant 1, recombinant protein (lane 2). Expected molecular weight - 17.6 kDa.
Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-FAS-1 Automated Western Blot (Cell Lysate)
Result: Negative
Automated western blot using CPTC-FAS-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) whole cell lysates. Expected molecular weight - 37.7 kDa, 11.4 kDa, 9.4 kDa, 16.6 kDa, 14.6 kDa, 35.4 kDa, 24.8 kDa. Buffy coat is positive. All cell lines are negative. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-FAS-1 Western Blot (Cell Lysate)
Result: Negative
Western blot using CPTC-FAS-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and H226 (lane 6) whole cell lysates. Expected molecular weight - 37.7 kDa, 11.4 kDa, 9.4 kDa, 16.6 kDa, 14.6 kDa, 35.4 kDa, 24.8 kDa. All cell lines are negative. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Background
NCI Identification Number:
00365
Antigen Name:
Fas Cell Surface Death Receptor Peptide 1
CPTC Name:
CPTC-FAS Peptide 1
Aliases:
Fas Cell Surface Death Receptor; Tumor Necrosis Factor Receptor Superfamily, Member 6; Fas (TNF Receptor Superfamily, Member 6); Apoptosis-Mediating Surface Antigen FAS; TNF Receptor Superfamily Member 6; FASLG Receptor; CD95 Antigen; TNFRSF6; APO-1; APT1; CD95; FAS1; Mutant Tumor Necrosis Receptor Superfamily Member 6; Apoptosis Signaling Receptor FAS; APO-1 Cell Surface Antigen; Apoptosis Antigen 1; Apo-1 Antigen; Fas AMA; ALPS1A; FASTM; FAS
Function:
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform.FAS (Fas Cell Surface Death Receptor) is a Protein Coding gene. Diseases associated with FAS include Autoimmune Lymphoproliferative Syndrome and Lymphoproliferative Syndrome. Among its related pathways are Apoptosis Modulation and Signaling and Signal transduction_JNK pathway. Gene Ontology (GO) annotations related to this gene include identical protein binding. An important paralog of this gene is TNFRSF10B.Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
Chromosomal Localization:
10q23.31
Accession Number:
NP_000034.1
UniProt Accession Number:
P25445
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
EA(pY)DTLIK
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
880
Last Updated:
09/02/2020
Links
- EnsEMBL - EnsEMBL FAS
- Entrez - Entrez FAS
- Uniprot - Uniprot P25445
Characterization Data
SOPs
No SOPs available.
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