Catalog Number:
CPTC-CTLA4-3
Target Antigen:
Cytotoxic T-Lymphocyte Associated Protein 4 Peptide 3
Isotype:
IgG
Species:
Rabbit Monoclonal Antibody
Last Updated:
11/21/2023
Antigen Recognition(s):
Peptide, Endogenous
Result: Positive
The affinity and binding kinetics of CPTC-CTLA4-3 antibody and BSA-conjugated peptide “AMDTGLYICK" were measured using biolayer interferometry. BSA-conjugated peptide was covalently immobilized onto AR2G biosensors using standard amine coupling. CPTC-CTLA4-3 antibody, 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, and 1 nM, was used as analyte. Buffer only and biosensors immobilized without BSA-conjugated peptide were used as references for background subtraction. All data was analyzed globally using a 1:2 bivalent model.
Result: Positive
The affinity and binding kinetics of CPTC-CTLA4-3 antibody and BSA-conjugated peptide, “AMDTGLYICK”, were measured using surface plasmon resonance. Peptide was amine coupled onto a Series S CM5 biosensor chip. Antibody at 256 nM, 64 nM, 16 nM, 4 nM, 1.0 nM and 0.25 nM, was used as analyte. Binding data were double-referenced and analyzed globally using a bivalent fitting model.
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Result: Positive
Immuno-MRM using CPTC-CTLA4-3 as capture antibody against the synthetic peptide AMDTGLYICK. Antibody CPTC-CTLA4-3 captures the synthetic peptide.
Result: Negative
Immunofluorescence staining using CPTC-CTLA4-3 as primary antibody against MCF7 cells shows no localization of CTLA4 protein.
Result: Positive
Indirect ELISA using CPTC-CTLA4-3 as primary antibody against BSA conjugated peptide, "AMDTGLYICK". Peptide was detected using HRP conjugated goat anti-rabbit secondary antibody and tetramethylbenzidine (TMB).
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Result: Negative
Western blot using CPTC-CTLA4-3 as primary antibody against human cytotoxic T-lymphocyte-associated protein 4 (CTLA4), transcript variant 1. The expected molecular weight is 16.0 kDa.
Result: Negative
Automated western blot using CPTC-CTLA4-3 as primary antibody against recombinant CTLA4 protein. Protein molecular weight is about 25 KDa, but the protein is glycosylated and runs at a higher molecular weight. The antibody cannot recognize recombinant CTLA4.
Result: Presumed Positive (with additional bands)
Western blot using CPTC-CTLA4-3 as primary antibody against whole cell lysates CCRF-CEM (lane 2), HeLa (lane 3), Jurkat (lane 4), K-562 (lane 5), and MCF7 (lane 6). Molecular weight standards are also included. The expected molecular weights are 24.7 kDa, 6.6 kDa, 6.7 kDa, 8.9 kDa, and 19.1 kDa. MCF7 is presumed positive. All other cell lines are negative. CTLA4 is subject to glycosylation which can affect its migration in electrophoresis. This can make the target appear as a higher molecular weight protein.
Result: Negative
Automated western blot using CPTC-CTLA4-2 as primary antibody against whole lysates of cell lines CCRF-CEM, HeLa, Jurkat, K-562 and MCF7. Protein molecular weight is about 25 KDa, but the protein is glycosylated and runs at a higher molecular weight. The antibody cannot recognize CTLA4 in the tested lysates. Loading controls were run with anti-GAPDH antibody.
Result: Negative
Single cell western blot using CPTC-CTLA4-3 as a primary antibody against MCF7 cell lysate. CTLA4 protein expression was not detected.
NCI Identification Number:
00518
Antigen Name:
Cytotoxic T-Lymphocyte Associated Protein 4 Peptide 3
CPTC Name:
CPTC-CTLA4 Peptide 3
Aliases:
Cytotoxic T-Lymphocyte Associated Protein 4; CTLA-4; CD152; GSE; CD; Insulin-Dependent Diabetes Mellitus 12; Cytotoxic T-Lymphocyte Protein 4; Celiac Disease 3; CELIAC3; IDDM12; Ligand And Transmembrane Spliced Cytotoxic T Lymphocyte Associated Antigen 4; Cytotoxic T Lymphocyte Associated Antigen 4 Short Spliced Form; Cytotoxic T-Lymphocyte-Associated Serine Esterase-4; Cytotoxic T-Lymphocyte-Associated Antigen 4; CD152 Antigen; ALPS5; GRD4
Function:
This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases.CTLA4 (Cytotoxic T-Lymphocyte Associated Protein 4) is a Protein Coding gene. Diseases associated with CTLA4 include Autoimmune Lymphoproliferative Syndrome, Type V and Celiac Disease 3. Among its related pathways are TCR Signaling (Qiagen) and T cell receptor signaling pathway. An important paralog of this gene is CD28.Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.
Chromosomal Localization:
2q33.2
Accession Number:
NP_005205.2
UniProt Accession Number:
P16410
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
AMDTGLYICK
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
1100
Last Updated:
05/31/2022
No SOPs available.
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