Background
Catalog Number:
CPTC-CSF2RA-1
Target Antigen:
Colony Stimulating Factor 2 Receptor Subunit Alpha Peptide 1
Isotype:
IgG
Species:
Rabbit Recombinant Cloned Antibody
Last Updated:
02/02/2024
Antigen Recognition(s):
Peptide, Recombinant Full-length
Purchase
- DSHB CPTC-CSF2RA-1
Characterization Data [Compare Characterization Data]
IHC HPA
Download This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org). (358.7 KB)
CPTC-CSF2RA-1 evaluation by the Human Protein Atlas
Result: Negative
This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org).
Characterization SOP Files
IHC Tissue
CPTC-CSF2RA-1 IHC Tissue
Result: Negative
This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.
Characterization SOP Files
NCI 60 Protein Array
CPTC-CSF2RA-1 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-CSF2RA-1 Western Blot Recombinant Protein
Result: Positive
Western blot using CPTC-CSF2RA-1 as primary antibody against human colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage) (CSF2RA), transcript variant 1, recombinant protein (lane 2). Expected molecular weight - 44.1 kDa. Molecular weight standards are also included (lane 1). Target protein is subject to glycosylation which can affect the migration in electrophoresis. This can make the target appear as a higher molecular weight protein.
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-CSF2RA-1 Automated Western Blot Cell Lysate
Result: Negative
Automated western blot using CPTC-CSF2RA-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 46.2 kDa, 46.9 kDa, 38.4 kDa, 32.9 kDa, 43.5 kDa, 26.6 kDa, 49.4 kDa, 31.1 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-CSF2RA-1 Western Blot Cell Lysate
Result: Negative
Western blot using CPTC-CSF2RA-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 46.2 kDa, 46.9 kDa, 38.4 kDa, 32.9 kDa, 43.5 kDa, 26.6 kDa, 49.9 kDa, and 31.1 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Background
NCI Identification Number:
00429
Antigen Name:
Colony Stimulating Factor 2 Receptor Subunit Alpha Peptide 1
CPTC Name:
CPTC-CSF2RA Peptide 1
Aliases:
Colony Stimulating Factor 2 Receptor Subunit Alpha; Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage); Granulocyte-Macrophage Colony-Stimulating Factor Receptor Subunit Alpha; Alpha-GM-CSF Receptor; GM-CSF-R-Alpha; CD116 Antigen; GMCSFR-Alpha; GMR-Alpha; AlphaGMR; CDw116; CSF2RY; CD116; CSF2R; Granulocyte-Macrophage Colony-Stimulating Factor Receptor Alpha Chain; Colony Stimulating Factor 2 Receptor Alpha Subunit; GM-CSF Receptor Alpha Subunit; CSF2RAX; CSF2RAY; CSF2RX; GMCSFR; CSF2RA; SMDP4; GMR
Function:
The protein encoded by this gene is the alpha subunit of the heterodimeric receptor for colony stimulating factor 2, a cytokine which controls the production, differentiation, and function of granulocytes and macrophages. The encoded protein is a member of the cytokine family of receptors. This gene is found in the pseudoautosomal region (PAR) of the X and Y chromosomes. Multiple transcript variants encoding different isoforms have been found for this gene, with some of the isoforms being membrane-bound and others being soluble.CSF2RA (Colony Stimulating Factor 2 Receptor Subunit Alpha) is a Protein Coding gene. Diseases associated with CSF2RA include Surfactant Metabolism Dysfunction, Pulmonary, 4 and Hereditary Pulmonary Alveolar Proteinosis. Among its related pathways are RET signaling and Signaling by GPCR. Gene Ontology (GO) annotations related to this gene include cytokine receptor activity. An important paralog of this gene is IL3RA.Low affinity receptor for granulocyte-macrophage colony-stimulating factor. Transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells.
Chromosomal Localization:
Accession Number:
NP_001155001.1
UniProt Accession Number:
P15509
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
NTQPGTENLLINVSGDLENR
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
2200
Last Updated:
09/21/2020
Links
- EnsEMBL - EnsEMBL CSF2RA
- Entrez - Entrez CSF2RA
- Uniprot - Uniprot P15509
Characterization Data
SOPs
No SOPs available.
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