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Chorionic Gonadotropin Subunit Beta Peptide 2

Antibody: CPTC-CGB-11
Background

Catalog Number:

CPTC-CGB-11

Target Antigen:

Chorionic Gonadotropin Subunit Beta Peptide 2

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

11/16/2021

Antigen Recognition(s):

Peptide

Characterization Data [Compare Characterization Data]
  Affinity Measurement by Biolayer Interferometry
Click to enlarge image The affinity and binding kinetics of CPTC-CGB-11 and BSA-conjugated Chorionic Gonadotropin Subunit Beta Peptide 2 (BSA-CDHPLT(CAM)DDRP) were measured using biolayer interferometry. CPTC-CGB-11 was covalently immobilized on amine-reactive second-generation sensors. BSA-conjugated peptide, 2.0 nM, 1.0 nM, 0.5 nM, 0.25 nM, 0.125 nM and 0.0625 nM, was used as analyte. Click image to enlarge

CPTC-CGB-11 Affinity and Kinetics

Result: Positive

The affinity and binding kinetics of CPTC-CGB-11 and BSA-conjugated Chorionic Gonadotropin Subunit Beta Peptide 2 (BSA-CDHPLT(CAM)DDRP) were measured using biolayer interferometry. CPTC-CGB-11 was covalently immobilized on amine-reactive second-generation sensors. BSA-conjugated peptide, 2.0 nM, 1.0 nM, 0.5 nM, 0.25 nM, 0.125 nM and 0.0625 nM, was used as analyte.


Characterization SOP Files

  Affinity Measurement by SPR

CPTC-CGB-11 Affinity and Kinetics

Result: Negative

Affinity and binding kinetics of CPTC-CGB-11 antibody and recombinant human hCG beta protein using surface plasmon resonance. CPTC-CGB-11 antibody was amine coupled onto a Series S CM5 biosensor chip. Recombinant human hCG beta protein, 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM, 0.25 nM and 0.0625 nM was used as analyte.


Characterization SOP Files

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Antibody: CPTC-CGB-4
Background

Catalog Number:

CPTC-CGB-4

Target Antigen:

Chorionic Gonadotropin Subunit Beta Peptide 2

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

07/08/2022

Antigen Recognition(s):

Peptide

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Characterization Data [Compare Characterization Data]
  Affinity Measurement by Biolayer Interferometry
Click to enlarge image The affinity and binding kinetics of CPTC-CGB-4 and BSA-conjugated Chorionic Gonadotropin Subunit Beta Peptide 2 (BSA-CDHPLT(CAM)DDRP) was measured using biolayer interferometry. CPTC-CGB-4 was covalently immobilized on amine-reactive second-generation sensors. BSA-conjugated peptide, 2.0 nM, 0.5 nM, 0.25 nM, 0.125 nM and 0.0625 nM, was used as analyte. Click image to enlarge

CPTC-CGB-4 Affinity and Kinetics

Result: Positive

The affinity and binding kinetics of CPTC-CGB-4 and BSA-conjugated Chorionic Gonadotropin Subunit Beta Peptide 2 (BSA-CDHPLT(CAM)DDRP) was measured using biolayer interferometry. CPTC-CGB-4 was covalently immobilized on amine-reactive second-generation sensors. BSA-conjugated peptide, 2.0 nM, 0.5 nM, 0.25 nM, 0.125 nM and 0.0625 nM, was used as analyte.


Characterization SOP Files

  Affinity Measurement by SPR

CPTC-CGB-4 Affinity and Kinetics

Result: Negative

Affinity and binding kinetics of CPTC-CGB-4 antibody and recombinant human hCG beta protein using surface plasmon resonance. CPTC-CGB-4 antibody was amine coupled onto a Series S CM5 biosensor chip. Recombinant human hCG beta protein, 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM, 0.25 nM and 0.0625 nM was used as analyte.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-CGB-4 as primary antibody against BSA-conjugated Chorionic Gonadotropin Subunit Beta Peptide 2 (BSA-CDHPLT(CAM)DDRP). Click image to enlarge

CPTC-CGB-4 Indirect ELISA

Result: Positive

Indirect ELISA using CPTC-CGB-4 as primary antibody against BSA-conjugated
Chorionic Gonadotropin Subunit Beta Peptide 2 (BSA-CDHPLT(CAM)DDRP).


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide

CPTC-CGB-4 Western Blot

Result: Negative

Western blot using CPTC-CGB-4 as primary antibody against human chorionic gonadotropin beta chain
(hCG beta) recombinant protein. Data not shown.


Characterization SOP Files

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Antibody: CPTC-CGB-5
Background

Catalog Number:

CPTC-CGB-5

Target Antigen:

Chorionic Gonadotropin Subunit Beta Peptide 2

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

11/16/2021

Antigen Recognition(s):

Peptide

Characterization Data [Compare Characterization Data]
  Affinity Measurement by Biolayer Interferometry
Click to enlarge image The affinity and binding kinetics of CPTC-CGB-5 and BSA-conjugated Chorionic Gonadotropin Subunit Beta Peptide 2 (BSA-CDHPLT(CAM)DDRP) was measured using biolayer interferometry. CPTC-CGB-5 was covalently immobilized on amine-reactive second-generation sensors. BSA-conjugated peptide, 2.0 nM, 1.0 nM, 0.25 nM, 0.125 nM and 0.0625 nM, was used as analyte. Click image to enlarge

CPTC-CGB-5 Affinity and Kinetics

Result: Positive

The affinity and binding kinetics of CPTC-CGB-5 and BSA-conjugated Chorionic Gonadotropin Subunit Beta Peptide 2 (BSA-CDHPLT(CAM)DDRP) was measured using biolayer interferometry. CPTC-CGB-5 was covalently immobilized on amine-reactive second-generation sensors. BSA-conjugated peptide, 2.0 nM, 1.0 nM, 0.25 nM, 0.125 nM and 0.0625 nM, was used as analyte.


Characterization SOP Files

  Affinity Measurement by SPR

CPTC-CGB-5 Affinity and Kinetics

Result: Negative

Affinity and binding kinetics of CPTC-CGB-5 antibody and recombinant human hCG beta protein using surface plasmon resonance. CPTC-CGB-5 antibody was amine coupled onto a Series S CM5 biosensor chip. Recombinant human hCG beta protein, 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM, 0.25 nM and 0.0625 nM was used as analyte.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-CGB-5 as primary antibody against BSA-conjugated Chorionic Gonadotropin Subunit Beta Peptide 2 (BSA-CDHPLT(CAM)DDRP). Click image to enlarge

CPTC-CGB-5 Indirect ELISA

Result: Positive

Indirect ELISA using CPTC-CGB-5 as primary antibody against BSA-conjugated Chorionic Gonadotropin Subunit Beta Peptide 2 (BSA-CDHPLT(CAM)DDRP).


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide

CPTC-CGB-5 Western Blot

Result: Negative

Western blot using CPTC-CGB-5 as primary antibody against human chorionic gonadotropin beta chain
(hCG beta) recombinant protein. Data not shown.


Characterization SOP Files

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Antibody: CPTC-CGB-6
Background

Catalog Number:

CPTC-CGB-6

Target Antigen:

Chorionic Gonadotropin Subunit Beta Peptide 2

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

09/01/2022

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

Characterization Data [Compare Characterization Data]
  Affinity Measurement by Biolayer Interferometry
Click to enlarge image The affinity and binding kinetics of CPTC-CGB-6 and BSA-conjugated Chorionic Gonadotropin Subunit Beta Peptide 2 (BSA-CDHPLT(CAM)DDRP) was measured using biolayer interferometry. CPTC-CGB-6 was covalently immobilized on amine-reactive second-generation sensors. BSA-conjugated peptide, 2.0 nM, 1.0 nM, 0.5 nM, 0.25 nM, 0.125 nM and 0.0625 nM, was used as analyte. Click image to enlarge

CPTC-CGB-6 Affinity and Kinetics

Result: Positive

The affinity and binding kinetics of CPTC-CGB-6 and BSA-conjugated Chorionic Gonadotropin Subunit Beta Peptide 2 (BSA-CDHPLT(CAM)DDRP) was measured using biolayer interferometry. CPTC-CGB-6 was covalently immobilized on amine-reactive second-generation sensors. BSA-conjugated peptide, 2.0 nM, 1.0 nM, 0.5 nM, 0.25 nM, 0.125 nM and 0.0625 nM, was used as analyte.


Characterization SOP Files

  Affinity Measurement by SPR

CPTC-CGB-6 Affinity and Kinetics

Result: Negative

Affinity and binding kinetics of CPTC-CGB-6 antibody and recombinant human hCG beta protein using surface plasmon resonance. CPTC-CGB-6 antibody was amine coupled onto a Series S CM5 biosensor chip. Recombinant human hCG beta protein, 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM, 0.25 nM and 0.0625 nM was used as analyte.


Characterization SOP Files

  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (509.8 KB)

CPTC-CGB-6 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immunofluorescence
Click to enlarge image Immunofluorescence staining of human cell line A549 with CPTC-CGB-6 Ab shows localization to the nucleus and cytosol. Click image to enlarge

CPTC-CGB-6

Result: Positive

Immunofluorescence staining of human cell line A549 with CPTC-CGB-6 Ab shows localization to the nucleus and cytosol.


Characterization SOP Files

  Immunofluorescence

CPTC-CGB-6 Immunofluorescence

Result: Negative

Immunofluorescence staining of HeLa and MCF7 cells using CPTC-CGB-6 antibody. CGB protein expression was not detected in either cell lines.


Characterization SOP Files

  Immunofluorescence - HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (509.8 KB)

CPTC-CGB-6 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-CGB-6 as primary antibody against BSA-conjugated Chorionic Gonadotropin Subunit Beta Peptide 2 (BSA-CDHPLT(CAM)DDRP). Click image to enlarge

CPTC-CGB-6 Indirect ELISA

Result: Positive

Indirect ELISA using CPTC-CGB-6 as primary antibody against BSA-conjugated Chorionic Gonadotropin Subunit Beta Peptide 2 (BSA-CDHPLT(CAM)DDRP).


Characterization SOP Files

  NCI 60 Protein Array

CPTC-CGB-6 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-CGB-6 as primary antibody against human chorionic gonadotropin beta chain (hCG beta) recombinant protein (lane 2). Expected molecular weight - 17 kDa. Molecular weight standards are also included (lane 1). Blot was developed using enhanced chemiluminescence (ECL). Click image to enlarge

CPTC-CGB-6 Western blot (Recombinant Protein)

Result: Positive

Western blot using CPTC-CGB-6 as primary antibody against human chorionic gonadotropin beta chain
(hCG beta) recombinant protein (lane 2). Expected molecular weight - 17 kDa. Molecular weight standards are also included (lane 1). Blot was developed using enhanced chemiluminescence (ECL).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-CGB-6 as primary antibody against A549 (lane 2), HeLa (lane 3), Jurkat (lane 4), MCF7 (lane 5), and H226 (lane 6) whole cell lysates. Expected molecular weight - 17 kDa. Molecular weight standards are also included (lane 1). Blot was developed using enhanced chemilumiescence (ECL). Click image to enlarge

CPTC-CGB-6 Western Blot (Cell Lysate)

Result: Positive

Western blot using CPTC-CGB-6 as primary antibody against A549 (lane 2), HeLa (lane 3), Jurkat (lane 4), MCF7 (lane 5), and H226 (lane 6) whole cell lysates. Expected molecular weight - 17 kDa. Molecular weight standards are also included (lane 1). Blot was developed using enhanced chemilumiescence (ECL).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-CGB-6 Single Cell Western Blot (Cell Lysate)

Result: Negative

Single cell western blot using CPTC-CGB-6 as a primary antibody against cell lysates. CGB protein was not detected and therefore no expression graphs were generated.


Characterization SOP Files

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Antigen: Chorionic Gonadotropin Subunit Beta Peptide 2
Background

NCI Identification Number:

00413

Antigen Name:

Chorionic Gonadotropin Subunit Beta Peptide 2

CPTC Name:

CPTC-CGB Peptide 2

Aliases:

Function:

This gene is a member of the glycoprotein hormone beta chain family and encodes the beta 3 subunit of chorionic gonadotropin (CG). Glycoprotein hormones are heterodimers consisting of a common alpha subunit and an unique beta subunit which confers biological specificity. CG is produced by the trophoblastic cells of the placenta and stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy. The beta subunit of CG is encoded by 6 genes which are arranged in tandem and inverted pairs on chromosome 19q13.3 and contiguous with the luteinizing hormone beta subunit gene.Beta subunit of the human chorionic gonadotropin (hCG). hCG is a complex glycoprotein composed of two glycosylated subunits alpha and beta which are non-covalently associated. The alpha subunit is identical to those in the pituitary gonadotropin hormones (LH, FSH and TSH). The beta subunits are distinct in each of the hormones and confer receptor and biological specificity. Has an essential role in pregnancy and maternal adaptation. Stimulates the ovaries to synthesize the steroids that are essential for the maintenance of pregnancy.

Chromosomal Localization:

Accession Number:

z

UniProt Accession Number:

P0DN86

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

DHPLTCDDPR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1100

Last Updated:

09/14/2020

Links

Characterization Data

SOPs

No SOPs available.

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