Background
Catalog Number:
CPTC-CD276-1
Target Antigen:
CD276 Molecule Peptide 1
Isotype:
IgG
Species:
Rabbit Monoclonal Antibody
Last Updated:
07/03/2024
Antigen Recognition(s):
Peptide, Endogenous
External Links
- CPTAC Assay Portal CPTC-CD276-1
Purchase
- DSHB CPTC-CD276-1
Characterization Data [Compare Characterization Data]
IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (355.6 KB)
CPTC-CPTC-CD276-1 Evaluation by the Human Protein Atlas
Result: Negative
This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)
Characterization SOP Files
Immuno-MRM
CPTC-CD276-1 iMRM (Frozen Tissue)
Result: Positive
Immuno-MRM chromatogram of CPTC-CD276-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6221)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue
Characterization SOP Files
NCI 60 Protein Array
CPTC-CD276-1 NCI 60 Protein Array
Result: Negative
This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.
Characterization SOP Files
Western Blot - Recombinant Protein or Peptide
CPTC-CD276-1 Western Blot Recombinant Protein
Result: Negative
Western blot using CPTC-CD276-1 as primary antibody against human CD276 molecule (CD276), transcript variant 2, recombinant protein (lane 2). Expected molecular weight - 33.6 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-CD276-1 Automated Western Blot Cell Lysate
Result: Negative
Automated western blot using CPTC-CD276-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weights - 57 kDa, 44 kDa, 53 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-CD276-1 Western Blot Cell Lysate
Result: Negative
Western blot using CPTC-CD276-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 57.2 kDa, 33.8 kDa, and 52.8 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Background
NCI Identification Number:
00420
Antigen Name:
CD276 Molecule Peptide 1
CPTC Name:
CPTC-CD276 Peptide 1
Aliases:
CD276 Molecule; CD276 Antigen; B7-H3; B7H3; Costimulatory Molecule; B7 Homolog 3; 4Ig-B7-H3; B7RP-2; CD276
Function:
The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.CD276 (CD276 Molecule) is a Protein Coding gene. Diseases associated with CD276 include Mycoplasma Pneumoniae Pneumonia and Brain Glioma. Among its related pathways are Cell adhesion molecules (CAMs) and NF-kappaB Signaling. Gene Ontology (GO) annotations related to this gene include signaling receptor binding. An important paralog of this gene is BTN1A1.May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.
Chromosomal Localization:
15q24.1
Accession Number:
NP_001019907.1
UniProt Accession Number:
Q5ZPR3
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
DLRPGDTVTITCSSYR
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
1760
Last Updated:
09/20/2020
Links
- EnsEMBL - EnsEMBL CD276
- Entrez - Entrez CD276
- Uniprot - Uniprot Q5ZPR3
Characterization Data
SOPs
No SOPs available.
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