CD276 Molecule Peptide 1


Background

Catalog Number:

CPTC-CD276-1

Target Antigen:

CD276 Molecule Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

07/03/2024

Antigen Recognition(s):

Peptide, Endogenous

External Links
Purchase
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (355.6 KB)

CPTC-CPTC-CD276-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-CD276-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6221)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue Click image to enlarge

CPTC-CD276-1 iMRM (Frozen Tissue)

Result: Positive

Immuno-MRM chromatogram of CPTC-CD276-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6221)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue


  NCI 60 Protein Array

CPTC-CD276-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


  Western Blot - Recombinant Protein or Peptide

CPTC-CD276-1 Western Blot Recombinant Protein

Result: Negative

Western blot using CPTC-CD276-1 as primary antibody against human CD276 molecule (CD276), transcript variant 2, recombinant protein (lane 2). Expected molecular weight - 33.6 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-CD276-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates.  Expected molecular weights - 57 kDa, 44 kDa, 53 kDa.  Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-CD276-1 Automated Western Blot Cell Lysate

Result: Negative

Automated western blot using CPTC-CD276-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weights - 57 kDa, 44 kDa, 53 kDa. Molecular weight standards are also included (lane 1).


  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-CD276-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates.  Expected molecular weight - 57.2 kDa, 33.8 kDa, and 52.8 kDa.  Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-CD276-1 Western Blot Cell Lysate

Result: Negative

Western blot using CPTC-CD276-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 57.2 kDa, 33.8 kDa, and 52.8 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

Background

NCI Identification Number:

00420

Antigen Name:

CD276 Molecule Peptide 1

CPTC Name:

CPTC-CD276 Peptide 1

Aliases:

CD276 Molecule; CD276 Antigen; B7-H3; B7H3; Costimulatory Molecule; B7 Homolog 3; 4Ig-B7-H3; B7RP-2; CD276

Function:

The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.CD276 (CD276 Molecule) is a Protein Coding gene. Diseases associated with CD276 include Mycoplasma Pneumoniae Pneumonia and Brain Glioma. Among its related pathways are Cell adhesion molecules (CAMs) and NF-kappaB Signaling. Gene Ontology (GO) annotations related to this gene include signaling receptor binding. An important paralog of this gene is BTN1A1.May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.

Chromosomal Localization:

15q24.1

Accession Number:

NP_001019907.1

UniProt Accession Number:

Q5ZPR3

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

DLRPGDTVTITCSSYR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1760

Last Updated:

09/20/2020

Links

Characterization Data

SOPs

No SOPs available.

Don't have Adobe Reader™?

Get it for free at Adobe.com