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BCL2 Associated X, Apoptosis Regulator Peptide 1

Antibody: CPTC-BAX-1
Background

Catalog Number:

CPTC-BAX-1

Target Antigen:

BCL2 Associated X, Apoptosis Regulator Peptide 1

Isotype:

IgG

Species:

Rabbit Recombinant Cloned Antibody

Last Updated:

02/09/2024

Antigen Recognition(s):

Peptide

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org). (338.3 KB)

CPTC-BAX-1 evaluation by the Human Protein Atlas

Result: Negative

This PDF provides the evaluation results as provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  NCI 60 Protein Array

CPTC-BAX-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide

CPTC-BAX-1 Western Blot Recombinant Protein

Result: Negative

Western blot using CPTC-BAX-1 as primary antibody against human BCL2-associated X protein (BAX), transcript variant alpha recombinant protein (lane 2). Expected molecular weight - 21.0 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-BAX-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 21 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-BAX-1 Automated Western Blot Cell Lysate

Result: Negative

Automated western blot using CPTC-BAX-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 21 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-BAX-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 21.2 kDa, 24.2 kDa, 4.7 kDa, 15.8 kDa, 18.1 kDa, 12.9 kDa, 19.3 kDa, and 19.7 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-BAX-1 Western Blot Cell Lysate

Result: Negative

Western blot using CPTC-BAX-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 21.2 kDa, 24.2 kDa, 4.7 kDa, 15.8 kDa, 18.1 kDa, 12.9 kDa, 19.3 kDa, and 19.7 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

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Antigen: BCL2 Associated X, Apoptosis Regulator Peptide 1
Background

NCI Identification Number:

00432

Antigen Name:

BCL2 Associated X, Apoptosis Regulator Peptide 1

CPTC Name:

CPTC-BAX Peptide 1

Aliases:

BCL2 Associated X, Apoptosis Regulator; BCL2L4; BCL2 Associated X Protein; Apoptosis Regulator BAX; Bcl-2-Like Protein 4; Bcl2-L-4; BCL2-Associated X Protein Omega; BCL2-Associated X Protein; Baxdelta2G9omega; Baxdelta2omega; Baxdelta2G9; BAX

Function:

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. The association and the ratio of BAX to BCL2 also determines survival or death of a cell following an apoptotic stimulus. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.BAX (BCL2 Associated X, Apoptosis Regulator) is a Protein Coding gene. Diseases associated with BAX include T-Cell Acute Lymphoblastic Leukemia and Colorectal Cancer. Among its related pathways are Apoptosis Modulation and Signaling and Transcriptional misregulation in cancer. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and protein heterodimerization activity. An important paralog of this gene is BOK.Plays a role in the mitochondrial apoptotic process. Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM) (PubMed:21458670). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.Bcl-2 family proteins contribute to programmed cell death or apoptosis. It is a large protein family and all members contain at least one of four Bcl-2 homology domains. Certain members (Bcl-2, Bcl-XL and Mcl-1) are antiapoptotic, whilst others (Bax, Bak, Bok) are proapoptotic.

Chromosomal Localization:

19q13.33

Accession Number:

NP_001278357.1

UniProt Accession Number:

Q07812

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

MIAAVDTDSPR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1210

Last Updated:

09/24/2020

Links

Characterization Data

SOPs

No SOPs available.

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