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Serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin) Member 6 Peptide 1

Antibody: CPTC-SERPINA6-1
Background

Catalog Number:

CPTC-SERPINA6-1

RRID:

AB_2617342

Target Antigen:

Serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin) Member 6 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

07/03/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (403.0 KB)

CPTC-SERPINA6-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-SERPINA6-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6225) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue Click image to enlarge

CPTC-SERPINA6-1 iMRM (Frozen Tissue)

Result: Positive

Immuno-MRM chromatogram of CPTC-SERPINA6-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6225)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to Peptide coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-SERPINA6-1 Indirect ELISA (peptide)

Result: Positive

Indirect ELISA (ie, binding of Antibody to Peptide coated plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-SERPINA6-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Over-expressed transient protein in cell lysate
Click to enlarge image Western Blot using CPTC-SERPINA6-1 as primary Ab against HEK293T cell lysate containing SERPINA6 (from Origene) in lane 2. Also included are molecular wt. standards (lane 1) and the SERPINA6-1 Ab as the IgG control (lane 3). Click image to enlarge

CPTC-SERPINA6-1 Western blot (cell lysate)

Result: Positive

Western Blot using CPTC-SERPINA6-1 as primary Ab against HEK293T cell lysate containing SERPINA6 (from Origene) in lane 2. Also included are molecular wt. standards (lane 1) and the SERPINA6-1 Ab as the IgG control (lane 3).


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide

CPTC-SERPINA6-1 Western Blot Recombinant Protein

Result: Negative

Western blot using CPTC-SERPINA6-1 as primary antibody against human serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 6 (SERPINA6) recombinant protein (lane 2). Expected molecular weight - 43.7 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-SERPINA6-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 45.1 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-SERPINA6-1 Automated Western Blot Cell Lysate

Result: Negative

Automated western blot using CPTC-SERPINA6-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 45.1 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-SERPINA6-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 45.1 kDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-SERPINA6-1 Western Blot Cell Lysate

Result: Negative

Western blot using CPTC-SERPINA6-1 as primary antibody against PBMC (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 45.1 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

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Antigen: Serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin) Member 6 Peptide 1
Background

NCI Identification Number:

00052

Antigen Name:

Serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin) Member 6 Peptide 1

CPTC Name:

CPTC-SERPINA6 Peptide 1

Aliases:

CBG; Serine (Or Cysteine) Proteinase Inhibitor, Clade A (Alpha-1 Antiproteinase,Antitrypsin), Member 6; Serpin A6; Corticosteroid Binding Globulin; Transcortin

Function:

This gene encodes an alpha-globulin protein with corticosteroid-binding properties. This is the major transport protein for glucorticoids and progestins in the blood of most vertebrates. The gene localizes to a chromosomal region containing several closely related serine protease inhibitors which may have evolved by duplication events.

Major transport protein for glucocorticoids and progestins in the blood of almost all vertebrate species.

Chromosomal Localization:

14q32.13

Accession Number:

NP_001747.2

UniProt Accession Number:

P08185

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

AQLLQGLGFNLTER

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

1540

Last Updated:

06/21/2013

Links

Characterization Data

SOPs

No SOPs available.

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