Background
Catalog Number:
CPTC-PTPN2-1
RRID:
AB_2890091
Target Antigen:
Protein Tyrosine Phosphatase Non-Receptor Type 2 Peptide 1
Isotype:
IgG
Species:
Rabbit Monoclonal Antibody
Last Updated:
05/22/2023
Antigen Recognition(s):
Peptide, Endogenous
Characterization Data [Compare Characterization Data]
Western Blot - Recombinant Protein or Peptide
CPTC-PTPN2-1 Western Blot (Recombinant Protein)
Result: Negative
Western blot using CPTC-PTPN2-1 as primary antibody against human tyrosine phosphatase, non-receptor type 2 (PTPN2),
transcript variant 3 recombinant protein (lane 2). Expected molecular weight - 40.8 kDa. Molecular weight standards are also included (lane 1).
Characterization SOP Files
Western Blot - Tissue or Cell Lysate
CPTC-PTPN2-1 Simple Western (tissue lysates)
Result: Positive
Automated WB (Simple Western) using CPTC-PTPN2-1 as primary antibody against the whole lysate of breast, ovary, spleen, endometrium and lung tissues. Expected MW is 48.5 KDa. The antibody recognizes the target in tested lysates. The same cell lysates were probed with an anti-CytC antibody (bottom panel).
Characterization SOP Files
Background
NCI Identification Number:
00390
Antigen Name:
Protein Tyrosine Phosphatase Non-Receptor Type 2 Peptide 1
CPTC Name:
CPTC-PTPN2 Peptide 1
Aliases:
Protein Tyrosine Phosphatase Non-Receptor Type 2; TCPTP; Tyrosine-Protein Phosphatase Non-Receptor Type 2; EC 3.1.3.48; TCELLPTP; TC-PTP; PTPT; T-Cell Protein Tyrosine Phosphatase; T-Cell Protein-Tyrosine Phosphatase; PTPN2; PTN2
Function:
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Epidermal growth factor receptor and the adaptor protein Shc were reported to be substrates of this PTP, which suggested the roles in growth factor mediated cell signaling. Multiple alternatively spliced transcript variants encoding different isoforms have been found. Two highly related but distinctly processed pseudogenes that localize to chromosomes 1 and 13, respectively, have been reported. PTPN2 (Protein Tyrosine Phosphatase Non-Receptor Type 2) is a Protein Coding gene. Diseases associated with PTPN2 include Oligoarticular Juvenile Idiopathic Arthritis and Rheumatoid Factor-Negative Polyarticular Juvenile Idiopathic Arthritis. Among its related pathways are Signaling by GPCR and Activation of cAMP-Dependent PKA. Gene Ontology (GO) annotations related to this gene include protein kinase binding and integrin binding. An important paralog of this gene is PTPN1.Non-receptor type tyrosine-specific phosphatase that dephosphorylates receptor protein tyrosine kinases including INSR, EGFR, CSF1R, PDGFR. Also dephosphorylates non-receptor protein tyrosine kinases like JAK1, JAK2, JAK3, Src family kinases, STAT1, STAT3 and STAT6 either in the nucleus or the cytoplasm. Negatively regulates numerous signaling pathways and biological processes like hematopoiesis, inflammatory response, cell proliferation and differentiation, and glucose homeostasis. Plays a multifaceted and important role in the development of the immune system. Functions in T-cell receptor signaling through dephosphorylation of FYN and LCK to control T-cells differentiation and activation. Dephosphorylates CSF1R, negatively regulating its downstream signaling and macrophage differentiation. Negatively regulates cytokine (IL2/interleukin-2 and interferon)-mediated signaling through dephosphorylation of the cytoplasmic kinases JAK1, JAK3 and their substrate STAT1, that propagate signaling downstream of the cytokine receptors. Also regulates the IL6/interleukin-6 and IL4/interleukin-4 cytokine signaling through dephosphorylation of STAT3 and STAT6 respectively. In addition to the immune system, it is involved in anchorage-dependent, negative regulation of EGF-stimulated cell growth. Activated by the integrin ITGA1/ITGB1, it dephosphorylates EGFR and negatively regulates EGF signaling. Dephosphorylates PDGFRB and negatively regulates platelet-derived growth factor receptor-beta signaling pathway and therefore cell proliferation. Negatively regulates tumor necrosis factor-mediated signaling downstream via MAPK through SRC dephosphorylation. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of the hepatocyte growth factor receptor MET. Plays also an important role in glucose homeostasis. For instance, negatively regulates the insulin receptor signaling pathway through the dephosphorylation of INSR and control gluconeogenesis and liver glucose production through negative regulation of the IL6 signaling pathways. May also bind DNA.
Chromosomal Localization:
18p11.21
Accession Number:
NP_002819.2
UniProt Accession Number:
P17706
DNA Source:
N/A
Immunogen:
Synthetic Peptide
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
LQNAENDYINASLVDIEEAQR
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
2310
Last Updated:
09/02/2020
Links
- CPTAC Assay Portal - EnsEMBL PTPN2
- CPTAC Assay Portal - Entrez PTPN2
- Uniprot - Uniprot P17706
Characterization Data
SOPs
No SOPs available.
Don't have Adobe Reader™?
Get it for free at Adobe.com