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Protein Tyrosine Phosphatase Non-Receptor Type 2 Peptide 1

Antibody: CPTC-PTPN2-1
Background

Catalog Number:

CPTC-PTPN2-1

RRID:

AB_2890091

Target Antigen:

Protein Tyrosine Phosphatase Non-Receptor Type 2 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

07/03/2024

Antigen Recognition(s):

Peptide, Endogenous

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Characterization Data [Compare Characterization Data]
  Affinity Measurement by SPR

CPTC-PTPN2-1 Affinity and Kinetics (Surface Plasmon Resonance)

Result: No Binding

Affinity and binding kinetics of CPTC-PTPN2-1 and full length PTPN2 recombinant protein were measured using surface plasmon resonance. CPTC-PTPN2-1 antibody was captured on a protein A biosensor chip. The recombinant protein at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM, and 0.25 nM, was used as analyte.


Characterization SOP Files

  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (509.6 KB)

CPTC-PTPN2-1 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  IP

CPTC-PTPN2-1 Immunoprecipitation

Result: Negative

Immuno-precipitation performed using CPTC-PTPN2-1 as capture antibody against the over-expressed lysate of PTPN2 (Myc-tagged). Eluates were tested in automated western blot using the an anti-Myc tag as detection antibody. The target protein was not pulled down.


Characterization SOP Files

  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-PTPN2-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6235) Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue Click image to enlarge

CPTC-PTPN2-1 iMRM (Frozen Tissue)

Result: Positive

Immuno-MRM chromatogram of CPTC-PTPN2-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-6235)
Data provided by the Paulovich Lab, Fred Hutch (https://research.fredhutch.org/paulovich/en.html). Data shown were obtained from frozen tissue


Characterization SOP Files

  Immunofluorescence

CPTC-PTPN2-1 Immunofluorescence

Result: Negative

Immunofluorescence staining of human cell line MCF7 with CPTC-PTPN2-1 Ab shows no localization of PTPN2 protein.


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (509.6 KB)

CPTC-PTPN2-1 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  NCI 60 Protein Array

CTPC-PTPN2-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide

CPTC-PTPN2-1 Western Blot (Recombinant Protein)

Result: Negative

Western blot using CPTC-PTPN2-1 as primary antibody against human tyrosine phosphatase, non-receptor type 2 (PTPN2),
transcript variant 3 recombinant protein (lane 2). Expected molecular weight - 40.8 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated western blot using CPTC-PTPN2-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 48.5 kDa, 45.2 kDa, 41.0 kDa, and 48.0 kDa. Molecular weight standards are also included (lane 1). Buffy coat is positive. All other cell lines are negative. Click image to enlarge

CPTC-PTPN2-1 Automated Western Blot Cell Lysate

Result: Positive

Automated western blot using CPTC-PTPN2-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and NCI-H226 (lane 7) whole cell lysates. Expected molecular weight - 48.5 kDa, 45.2 kDa, 41.0 kDa, and 48.0 kDa. Molecular weight standards are also included (lane 1). Buffy coat is positive. All other cell lines are negative.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-PTPN2-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and NCI-H226 (lane 6) whole cell lysates. Expected molecular weight - 48.5 kDa, 45.2 kDa, 41.0 kDa, and 48.0 kDa. Molecular weight standards are also included (lane 1). All cell lines are positive. Click image to enlarge

CPTC-PTPN2-1 Western Blot Cell Lysate

Result: Positive

Western blot using CPTC-PTPN2-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5), and NCI-H226 (lane 6) whole cell lysates. Expected molecular weight - 48.5 kDa, 45.2 kDa, 41.0 kDa, and 48.0 kDa. Molecular weight standards are also included (lane 1). All cell lines are positive.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-PTPN2-1 Single Cell Western Blot (Cell Lysate)

Result: Negative

Single cell western blot using CPTC-PTPN2-1 as a primary antibody against MCF7 cell lysate. PTPN2 protein expression was not detected.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-PTPN2-1 Western Blot (Tissue Lysates)

Result: Negative

Western blot using CPTC-PTPN2-1 as primary antibody against human breast (2), ovary (3), spleen (4), endometrium (5), and lung (6) tissue lysates. The expected molecular weight is 48.5 kDa. Cytochrome C was used as a loading control.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated WB (Simple Western) using CPTC-PTPN2-1 as primary antibody against the whole lysate of breast, ovary, spleen, endometrium and lung tissues. Expected MW is 48.5 KDa. The antibody recognizes the target in tested lysates. The same cell lysates were probed with an anti-CytC antibody (bottom panel). Click image to enlarge

CPTC-PTPN2-1 Simple Western (tissue lysates)

Result: Positive

Automated WB (Simple Western) using CPTC-PTPN2-1 as primary antibody against the whole lysate of breast, ovary, spleen, endometrium and lung tissues. Expected MW is 48.5 KDa. The antibody recognizes the target in tested lysates. The same cell lysates were probed with an anti-CytC antibody (bottom panel).


Characterization SOP Files

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Antigen: Protein Tyrosine Phosphatase Non-Receptor Type 2 Peptide 1
Background

NCI Identification Number:

00390

Antigen Name:

Protein Tyrosine Phosphatase Non-Receptor Type 2 Peptide 1

CPTC Name:

CPTC-PTPN2 Peptide 1

Aliases:

Protein Tyrosine Phosphatase Non-Receptor Type 2; TCPTP; Tyrosine-Protein Phosphatase Non-Receptor Type 2; EC 3.1.3.48; TCELLPTP; TC-PTP; PTPT; T-Cell Protein Tyrosine Phosphatase; T-Cell Protein-Tyrosine Phosphatase; PTPN2; PTN2

Function:

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Epidermal growth factor receptor and the adaptor protein Shc were reported to be substrates of this PTP, which suggested the roles in growth factor mediated cell signaling. Multiple alternatively spliced transcript variants encoding different isoforms have been found. Two highly related but distinctly processed pseudogenes that localize to chromosomes 1 and 13, respectively, have been reported. PTPN2 (Protein Tyrosine Phosphatase Non-Receptor Type 2) is a Protein Coding gene. Diseases associated with PTPN2 include Oligoarticular Juvenile Idiopathic Arthritis and Rheumatoid Factor-Negative Polyarticular Juvenile Idiopathic Arthritis. Among its related pathways are Signaling by GPCR and Activation of cAMP-Dependent PKA. Gene Ontology (GO) annotations related to this gene include protein kinase binding and integrin binding. An important paralog of this gene is PTPN1.Non-receptor type tyrosine-specific phosphatase that dephosphorylates receptor protein tyrosine kinases including INSR, EGFR, CSF1R, PDGFR. Also dephosphorylates non-receptor protein tyrosine kinases like JAK1, JAK2, JAK3, Src family kinases, STAT1, STAT3 and STAT6 either in the nucleus or the cytoplasm. Negatively regulates numerous signaling pathways and biological processes like hematopoiesis, inflammatory response, cell proliferation and differentiation, and glucose homeostasis. Plays a multifaceted and important role in the development of the immune system. Functions in T-cell receptor signaling through dephosphorylation of FYN and LCK to control T-cells differentiation and activation. Dephosphorylates CSF1R, negatively regulating its downstream signaling and macrophage differentiation. Negatively regulates cytokine (IL2/interleukin-2 and interferon)-mediated signaling through dephosphorylation of the cytoplasmic kinases JAK1, JAK3 and their substrate STAT1, that propagate signaling downstream of the cytokine receptors. Also regulates the IL6/interleukin-6 and IL4/interleukin-4 cytokine signaling through dephosphorylation of STAT3 and STAT6 respectively. In addition to the immune system, it is involved in anchorage-dependent, negative regulation of EGF-stimulated cell growth. Activated by the integrin ITGA1/ITGB1, it dephosphorylates EGFR and negatively regulates EGF signaling. Dephosphorylates PDGFRB and negatively regulates platelet-derived growth factor receptor-beta signaling pathway and therefore cell proliferation. Negatively regulates tumor necrosis factor-mediated signaling downstream via MAPK through SRC dephosphorylation. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of the hepatocyte growth factor receptor MET. Plays also an important role in glucose homeostasis. For instance, negatively regulates the insulin receptor signaling pathway through the dephosphorylation of INSR and control gluconeogenesis and liver glucose production through negative regulation of the IL6 signaling pathways. May also bind DNA.

Chromosomal Localization:

18p11.21

Accession Number:

NP_002819.2

UniProt Accession Number:

P17706

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

LQNAENDYINASLVDIEEAQR

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

2310

Last Updated:

09/02/2020

Links

Characterization Data

SOPs

No SOPs available.

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