Proteasome 20S Subunit Alpha 1 Peptide 1


Background

Catalog Number:

CPTC-PSMA1-1

RRID:

AB_2889059

Target Antigen:

Proteasome 20S Subunit Alpha 1 Peptide 1

Isotype:

IgG

Species:

Mouse Monoclonal Antibody

Last Updated:

07/12/2022

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

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Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (420.9 KB)

CPTC-PSMA1-1 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


  IHC Tissue
Click to enlarge image Tissue Micro-Array (TMA) core of lung cancer  showing cytoplasmic and nuclear staining using Antibody CPTC-PSMA1-1. Titer: 1:40000 Click image to enlarge

CPTC-PSMA1-1 IHC Tissue

Result: Positive

Tissue Micro-Array (TMA) core of lung cancer showing cytoplasmic and nuclear staining using Antibody CPTC-PSMA1-1. Titer: 1:40000


  Immunofluorescence
Click to enlarge image Immunofluorescence staining of human cell line Jurkat using CPTC-PSMA1-1 antibody (green). PSMA1 protein expression shows localization to the nucleoplasm. CPTC-PSMA1-1 antibody showed similar results with MCF7 and NCI H226 cell lines (data not shown). Click image to enlarge

CPTC-PSMA1-1 Immunofluorescence

Result: Positive

Immunofluorescence staining of human cell line Jurkat using CPTC-PSMA1-1 antibody (green). PSMA1 protein expression shows localization to the nucleoplasm. CPTC-PSMA1-1 antibody showed similar results with MCF7 and NCI H226 cell lines (data not shown).


  NCI 60 Protein Array

CTPC-PSMA1-1 NCI60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


  Western Blot - Cell Lysate
Click to enlarge image Automated western blot using CPTC-PSMA1-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) cell lysates.  Expected molecular weight - 29 kDa.  Molecular weight standards are also included (lane 1). Data are negative/inconclusive for all cell lines. Click image to enlarge

CPTC-PSMA1-1 Automated Western Blot (Cell Lysate)

Result: Negative

Automated western blot using CPTC-PSMA1-1 as primary antibody against buffy coat (lane 2), HeLa (lane 3), Jurkat (lane 4), A549 (lane 5), MCF7 (lane 6), and H226 (lane 7) cell lysates. Expected molecular weight - 29 kDa. Molecular weight standards are also included (lane 1). Data are negative/inconclusive for all cell lines.


  Western Blot - Cell Lysate
Click to enlarge image Western blot using CPTC-PSMA1-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5) and NCI H226 (lane 6) cell lysates.  Expected molecular weight 30 kDa.  Molecular weight standards (MW Stds.) are also included (lane 1).  Positive for cell lines HeLa, Jurkat, A549, MCF7 and NCI H226. Click image to enlarge

CPTC-PSMA1-1 Western Blot (Cell Lysate)

Result: Positive

Western blot using CPTC-PSMA1-1 as primary antibody against HeLa (lane 2), Jurkat (lane 3), A549 (lane 4), MCF7 (lane 5) and NCI H226 (lane 6) cell lysates. Expected molecular weight 30 kDa. Molecular weight standards (MW Stds.) are also included (lane 1). Positive for cell lines HeLa, Jurkat, A549, MCF7 and NCI H226.


Characterization SOP Files

  Western Blot - Cell Lysate

CPTC-PSMA1-1 Single Cell Western Blot (Cell Lysate)

Result: Negative

Single cell western blot using CPTC-PSMA1-1 as a primary antibody against MCF7, NCI H226 and Jurkat cell lysates. PSMA1 protein expression was not detected.


  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-PSMA1-1 as primary antibody against human proteasome (prosome, macropain) subunit, alpha type, 1 (PSMA1), transcript variant 2 recombinant protein (lane 2). Expected molecular weight - 28.9 kDa.  Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-PSMA1-1 Western Blot (recombinant protein)

Result: Positive

Western blot using CPTC-PSMA1-1 as primary antibody against human proteasome (prosome, macropain) subunit, alpha type, 1 (PSMA1), transcript variant 2 recombinant protein (lane 2). Expected molecular weight - 28.9 kDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

Background

NCI Identification Number:

00388

Antigen Name:

Proteasome 20S Subunit Alpha 1 Peptide 1

CPTC Name:

CPTC-PSMA1 Peptide 1

Aliases:

Proteasome 20S Subunit Alpha 1; PROS30; HC2; NU; Proteasome (Prosome, Macropain) Subunit, Alpha Type, 1; Multicatalytic Endopeptidase Complex Subunit C2; Proteasome Subunit Alpha Type-1; Proteasome Subunit Alpha 1; 30 KDa Prosomal Protein; Proteasome Component C2; Macropain Subunit C2; Proteasome Nu Chain; EC 3.4.25.1; PROS-30; Epididymis Secretory Protein Li 275; Proteasome Subunit, Alpha-Type, 1; Testicular Tissue Protein Li 150; Proteasome Subunit Alpha 6; Proteasome Subunit A6; Proteasome Subunit Nu; Macropain Subunit Nu; Protein P30-33K; HEL-S-275; MGC14542; MGC14575; MGC14751; MGC21459; MGC22853; MGC23915; MGC1667; PSMA1; PSC2

Function:

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Alternative splicing results in multiple transcript variants encoding distinct isoforms.PSMA1 (Proteasome 20S Subunit Alpha 1) is a Protein Coding gene. Diseases associated with PSMA1 include Cystic Fibrosis and Alzheimer Disease. Among its related pathways are Regulation of activated PAK-2p34 by proteasome mediated degradation and Antigen processing-Cross presentation. Gene Ontology (GO) annotations related to this gene include RNA binding and threonine-type endopeptidase activity. An important paralog of this gene is ENSG00000256206.Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).

Chromosomal Localization:

11p15.2

Accession Number:

NP_683877.1

UniProt Accession Number:

P25786

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

ETLPAEQDLTTK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1320

Last Updated:

09/02/2020

Links

Characterization Data

SOPs

No SOPs available.

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