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Prominin 1 Peptide 1

Antibody: CPTC-PROM1-1
Background

Catalog Number:

CPTC-PROM1-1

Target Antigen:

Prominin 1 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

06/01/2021

Antigen Recognition(s):

Recombinant Full-length

Thematic Panel(s):

Epithelial Mesenchymal Transition (EMT)

Publications
Characterization Data [Compare Characterization Data]
  Immunofluorescence

CPTC-PROM1-1

Result: Negative

Immunofluorescence staining of human cell line HT-29 with CPTC-PROM1-1 Ab shows no localization of PROM1 protein.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-PROM1-1 as primary antibody against PROM1 protein domain comprising amino acids 180-400 (lane 2) with expected MW of 27 KDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-PROM1-1 WB

Result: Positive

Western Blot using CPTC-PROM1-1 as primary antibody against PROM1 protein domain comprising amino acids 180-400 (lane 2) with expected MW of 27 KDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-PROM1-1 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 97 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-PROM1-1 Endogenous Western Blot

Result: Negative

Western Blot using CPTC-PROM1-1 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 97 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated Western Blot using CPTC-PROM1-1 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 97 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-PROM1-1 Endogenous Automated Western Blot

Result: Negative

Automated Western Blot using CPTC-PROM1-1 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 97 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Single cell western blot using CPTC-PROM1-1 as a primary antibody against cell lysates. Relative expression of total PROM1 in HT-29 and U87MG cells (A). Percentage of cells that express PROM1 (B). Average expression of PROM1 protein per cell (C). All data is normalized to β-tubulin expression. Click image to enlarge

CPTC-PROM1-1 Single Cell Western Blot (Cell Lysate)

Result: Positive

Single cell western blot using CPTC-PROM1-1 as a primary antibody against cell lysates. Relative expression of total PROM1 in HT-29 and U87MG cells (A). Percentage of cells that express PROM1 (B). Average expression of PROM1 protein per cell (C). All data is normalized to β-tubulin expression.


Characterization SOP Files

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Antigen: Prominin 1 Peptide 1
Background

NCI Identification Number:

00265

Antigen Name:

Prominin 1 Peptide 1

CPTC Name:

CPTC-PROM1 Peptide 1

Aliases:

Prominin 1; Prominin-Like Protein 1; Antigen AC133; PROML1; Stargardt Disease 4 (Autosomal Dominant); Hematopoietic Stem Cell Antigen; Macular Dystrophy, Retinal 2; Prominin (Mouse)-Like 1; CD133 Antigen; Prominin-1; HProminin; MSTP061; CORD12; AC133; CD133; MCDR2; STGD4; RP41

Function:

This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene.

GeneCards Summary for PROM1 Gene
PROM1 (Prominin 1) is a Protein Coding gene. Diseases associated with PROM1 include Macular Dystrophy, Retinal, 2 and Stargardt Disease 4. Among its related pathways are Wnt / Hedgehog / Notch and Embryonic and Induced Pluripotent Stem Cell Differentiation Pathways and Lineage-specific Markers. Gene Ontology (GO) annotations related to this gene include cadherin binding and actinin binding. An important paralog of this gene is PROM2.

May play a role in cell differentiation, proliferation and apoptosis (PubMed:24556617). Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner (PubMed:20818439).

Chromosomal Localization:

4p15.32

Accession Number:

NP_001139319.1

UniProt Accession Number:

O43490

DNA Source:

The NCI acknowledges the generous advice and gene clones provided by Robert A. Weinberg at the Ludwig Center for Cancer Research of the Massachusetts Institute of Technology. These reagents have been provided by the Division of Cancer Treatment and Diagnosis (DCTD) of the National Cancer Institute (NCI) using funding of the American Recovery and Reinvestment Act of 2009

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

KTSLRSSLNDPLCLVHPSSETCNSIRLSLSQLNSN

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

3850

Last Updated:

09/16/2019

Links

Characterization Data

SOPs

No SOPs available.

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