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Neurofibromin 1

Antibody: CPTC-NF1-1
Background

Catalog Number:

CPTC-NF1-1

RRID:

AB_2920914

Target Antigen:

Neurofibromin 1

Isotype:

IgG3

Species:

Mouse Monoclonal Antibody

Last Updated:

07/01/2025

Antigen Recognition(s):

Recombinant Full-length, Endogenous

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Characterization Data [Compare Characterization Data]
  Affinity Measurement by Biolayer Interferometry

CPTC-NF1-1 Affinity and Kinetics

Result: No Binding

Affinity and binding kinetics of CPTC-NF1-1 antibody and NF1 recombinant protein using biolayer interferometry. CPTC-NF1-1 antibody was covalently immobilized on amine-reactive second-generation sensors. NF1 recombinant protein, 64 nM, 16 nM, 4.0 nM, 1 nM, 0.25 nM, 0.0625 nM and 0.0156 nM, was used as analyte. NF1 recombinant protein showed strong non-specific binding to the AR2G reference biosensors that were not immobilized with antibody. This binding prevented accurate calculation of both affinity and kinetics parameters.


Characterization SOP Files

  Affinity Measurement by SPR
Click to enlarge image Affinity and binding kinetics of CPTC-NF1-1 antibody and NF1 recombinant protein using surface plasmon resonance. CPTC-NF1-1 antibody was captured onto a Series S Protein G biosensor chip. NF1 recombinant protein, 1024 nM, 256 nM, 16 nM, 4 nM and 1.0 nM, was used as analyte. Kinetic constant Ka is approaching the limits that can be measured by the instrument. Click image to enlarge

CPTC-NF1-1 Affinity and Kinetics

Result: High Binding

Affinity and binding kinetics of CPTC-NF1-1 antibody and NF1 recombinant protein using surface plasmon resonance. CPTC-NF1-1 antibody was captured onto a Series S Protein G biosensor chip. NF1 recombinant protein, 1024 nM, 256 nM, 16 nM, 4 nM and 1.0 nM, was used as analyte. Kinetic constant Ka is approaching the limits that can be measured by the instrument.


Characterization SOP Files

  CyTOF
Click to enlarge image Imaging mass cytometry on colon cancer tissue core using CPTC-NF1-1 metal-labeled antibody. Data shows overlay of target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (testis, prostate, colon, appendix, pancreas, and kidney) and cancer tissues (breast, colon, lung, and ovarian). Click image to enlarge

CPTC-NF1-1 Immuno-Mass Cytometry

Result: Positive

Imaging mass cytometry on colon cancer tissue core using CPTC-NF1-1 metal-labeled antibody. Data shows overlay of target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (testis, prostate, colon, appendix, pancreas, and kidney) and cancer tissues (breast, colon, lung, and ovarian).


Characterization SOP Files

  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (455.6 KB)

CPTC-NF1-1 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Micro-Array (TMA) core of prostate showing cytoplasmic and membranous staining using Antibody CPTC-NF1-1. Titer: 1:200 Click image to enlarge

CPTC-NF1-1 IHC Tissue

Result: Positive

Tissue Micro-Array (TMA) core of prostate showing cytoplasmic and membranous staining using Antibody CPTC-NF1-1. Titer: 1:200


Characterization SOP Files

  IP

CPTC-NF1-1 IP-WB

Result: Negative

IP-WB performed with CPTC-NF1-1 as capture antibody against recombinant protein and MCF7 lysate. Eluates were screened in WB using antibody CPTC-NF1-1 as detection antibody. This antibody is not suitable for use in this application.


Characterization SOP Files

  Immunofluorescence

CPTC-NF1-1 Immunofluorescence

Result: Negative

Immunofluorescence staining of human cell line MCF7 with CPTC-NF1-1 Ab shows no localization of NF1 protein.


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (455.6 KB)

CPTC-NF1-1 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-NF1-1 as primary antibody against NF1 recombinant protein. Click image to enlarge

CPTC-NF1-1 Indirect ELISA

Result: Medium Low Binding

Indirect ELISA using CPTC-NF1-1 as primary antibody against NF1 recombinant protein.


Characterization SOP Files

  NCI 60 Protein Array
Click to enlarge image Protein Array in which CPTC-NF1-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

CPTC-NF1-1 NCI Protein Array

Result: Positive

Protein Array in which CPTC-NF1-1 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-NF1-1 as primary antibody against recombinant protein NF1. Expected MW is above 250 KDa. 7% gel. Click image to enlarge

CPTC-NF1-1 WB (Recombinant Protein)

Result: Presumed Positive (with additional bands)

Western Blot using CPTC-NF1-1 as primary antibody against recombinant protein NF1. Expected MW is above 250 KDa.
7% gel.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-NF1-1 as primary antibody against cell lysates of HeLa, Jurkat, A549, MCF7 and H226. Presumed positve for MCF7. Expected MW is above 250 KDa. Click image to enlarge

CPTC-NF-1 WB (Lysates)

Result: Presumed Positive (with additional bands)

Western Blot using CPTC-NF1-1 as primary antibody against cell lysates of HeLa, Jurkat, A549, MCF7 and H226. Presumed positve for MCF7. Expected MW is above 250 KDa.


Characterization SOP Files

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Antibody: CPTC-NF1-2
Background

Catalog Number:

CPTC-NF1-2

RRID:

AB_2920915

Target Antigen:

Neurofibromin 1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

07/01/2025

Antigen Recognition(s):

Recombinant Full-length, Endogenous

Purchase
Characterization Data [Compare Characterization Data]
  Affinity Measurement by Biolayer Interferometry

CPTC-NF1-2 Affinity and Kinetics

Result: No Binding

Affinity and binding kinetics of CPTC-NF1-2 antibody and NF1 recombinant protein using biolayer interferometry. CPTC-NF1-2 antibody was covalently immobilized on amine-reactive second-generation sensors. NF1 recombinant protein, 64 nM, 16 nM, 4.0 nM, 1 nM, 0.25 nM, 0.0625 nM and 0.0156 nM, was used as analyte. NF1 recombinant protein showed strong non-specific binding to the AR2G reference biosensors that were not immobilized with antibody. This binding prevented accurate calculation of both affinity and kinetics parameters.


Characterization SOP Files

  Affinity Measurement by SPR

CPTC-NF1-2 Affinity and Kinetics

Result: No Binding

Affinity and binding kinetics of CPTC-NF1-2 antibody and NF1 recombinant protein using surface plasmon resonance. CPTC-NF1-2 antibody was captured onto a Series S Protein G biosensor chip. NF1 recombinant protein, 256 nM, 16 nM, 4 nM, 1 nM and 0.25 nM, was used as analyte.


Characterization SOP Files

  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (390.6 KB)

CPTC-NF1-2 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Micro-Array (TMA) core of breast cancer showing cytoplasmic and membranous staining using Antibody CPTC-NF1-2. Titer: 1:50 Click image to enlarge

CPTC-NF1-2 IHC Tissue

Result: Positive

Tissue Micro-Array (TMA) core of breast cancer showing cytoplasmic and membranous staining using Antibody CPTC-NF1-2. Titer: 1:50


Characterization SOP Files

  IP

CPTC-NF1-2 IP-WB

Result: Negative

IP-WB performed with CPTC-NF1-2 as capture antibody against recombinant protein and MCF7 lysate. Eluates were screened in WB using antibody CPTC-NF1-1 as detection antibody. This antibody is not suitable for use in this application.


Characterization SOP Files

  Immunofluorescence

CPTC-NF1-2 Immunofluorescence

Result: Negative

Immunofluorescence staining of human cell line MCF7 with CPTC-NF1-2 Ab shows no localization of NF1 protein.


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (390.6 KB)

CPTC-NF1-2 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-NF1-2 as primary antibody against NF1 recombinant protein. Click image to enlarge

CPTC-NF1-2 Indirect ELISA

Result: Medium Low Binding

Indirect ELISA using CPTC-NF1-2 as primary antibody against NF1 recombinant protein.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-NF1-2

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-NF1-2 as primary antibody against recombinant protein NF1. Expected MW is above 250 KDa. 7% gel. Click image to enlarge

CPTC-NF1-2 WB (Recombinant Protein)

Result: Presumed Positive (with additional bands)

Western Blot using CPTC-NF1-2 as primary antibody against recombinant protein NF1. Expected MW is above 250 KDa. 7% gel.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-NF1-2 as primary antibody against cell lysates of HeLa, Jurkat, A549, MCF7 and H226.. Expected MW is above 250 KDa. 7% gel. Click image to enlarge

CPTC-NF-2 WB (Lysates)

Result: Negative

Western Blot using CPTC-NF1-2 as primary antibody against cell lysates of HeLa, Jurkat, A549, MCF7 and H226.. Expected MW is above 250 KDa. 7% gel.


Characterization SOP Files

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Antibody: CPTC-NF1-3
Background

Catalog Number:

CPTC-NF1-3

RRID:

AB_2920916

Target Antigen:

Neurofibromin 1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

07/01/2025

Antigen Recognition(s):

Recombinant Full-length

Purchase
Characterization Data [Compare Characterization Data]
  Affinity Measurement by Biolayer Interferometry

CPTC-NF1-3 Affinity and Kinetics

Result: No Binding

Affinity and binding kinetics of CPTC-NF1-3 antibody and NF1 recombinant protein using biolayer interferometry. CPTC-NF1-3 antibody was covalently immobilized on amine-reactive second-generation sensors. NF1 recombinant protein, 64 nM, 16 nM, 4.0 nM, 1 nM, 0.25 nM, 0.0625 nM and 0.0156 nM, was used as analyte. NF1 recombinant protein showed strong non-specific binding to the AR2G reference biosensors that were not immobilized with antibody. This binding prevented accurate calculation of both affinity and kinetics parameters.


Characterization SOP Files

  Affinity Measurement by SPR

CPTC-NF1-3 Affinity and Kinetics

Result: No Binding

Affinity and binding kinetics of CPTC-NF1-3 antibody and NF1 recombinant protein using surface plasmon resonance. CPTC-NF1-3 antibody was captured onto a Series S Protein G biosensor chip. NF1 recombinant protein, 16 nM, 4 nM, 1.0 nM, 0.25 nM and 0.0625 nM, was used as analyte.


Characterization SOP Files

  IHC HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (379.1 KB)

CPTC-NF1-3 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  IHC Tissue

CPTC-NF1-3 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  IP
Click to enlarge image IP-WB performed with CPTC-NF1-3 as capture antibody against recombinant protein and MCF7 lysate. Eluates were screened in WB using antibody CPTC-NF1-1 as detection antibody. CPTC-NF1-3 I able to pull down the target protein in MCF7 cell lysates. Results from recombinant protein pull down are inconclusive. NF1 expected MW is 319 KDa. Click image to enlarge

CPTC-NF1-3 IP-WB

Result: Uncertain

IP-WB performed with CPTC-NF1-3 as capture antibody against recombinant protein and MCF7 lysate. Eluates were screened in WB using antibody CPTC-NF1-1 as detection antibody. CPTC-NF1-3 I able to pull down the target protein in MCF7 cell lysates. Results from recombinant protein pull down are inconclusive. NF1 expected MW is 319 KDa.


Characterization SOP Files

  Immunofluorescence

CPTC-NF1-3 Immunofluorescence

Result: Negative

Immunofluorescence staining of human cell line MCF7 with CPTC-NF1-3 Ab shows no localization of NF1 protein.


Characterization SOP Files

  Immunofluorescence - HPA
Download Results provided by the Human Protein Atlas (www.proteinatlas.org). (379.1 KB)

CPTC-NF1-3 evaluation by the Human Protein Atlas

Result: Negative

Results provided by the Human Protein Atlas (www.proteinatlas.org).


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-NF1-3 as primary antibody against NF1 recombinant protein. Click image to enlarge

CPTC-NF1-3 Indirect ELISA

Result: Medium Low Binding

Indirect ELISA using CPTC-NF1-3 as primary antibody against NF1 recombinant protein.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-NF1-3 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-NF1-3 as primary antibody against recombinant protein NF1. Expected MW is above 250 KDa. 7% gel. Click image to enlarge

CPTC-NF1-3 WB (Recombinant Protein)

Result: Presumed Positive (with additional bands)

Western Blot using CPTC-NF1-3 as primary antibody against recombinant protein NF1. Expected MW is above 250 KDa. 7% gel.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-NF1-3 as primary antibody against cell lysates of HeLa, Jurkat, A549, MCF7 and H226. Presumed positve for MCF7. Expected MW is above 250 KDa. 7% gel. Click image to enlarge

CPTC-NF-3 WB (Lysates)

Result: Presumed Positive (with additional bands)

Western Blot using CPTC-NF1-3 as primary antibody against cell lysates of HeLa, Jurkat, A549, MCF7 and H226. Presumed positve for MCF7. Expected MW is above 250 KDa. 7% gel.


Characterization SOP Files

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Antigen: Neurofibromin 1
Background

NCI Identification Number:

00489

Antigen Name:

Neurofibromin 1

CPTC Name:

CPTC-NF1

Aliases:

Neurofibromin 1; Neurofibromatosis-Related Protein NF-1; Neurofibromin; Von Recklinghausen Disease; Truncated Neurofibromin 1; Neurofibromatosis 1; Neurofibromatosis; Watson Disease; NFNS; VRNF; WSS; NF1

Function:

This gene product appears to function as a negative regulator of the ras signal transduction pathway. Mutations in this gene have been linked to neurofibromatosis type 1, juvenile myelomonocytic leukemia and Watson syndrome. The mRNA for this gene is subject to RNA editing (CGA>UGA->Arg1306Term) resulting in premature translation termination. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene.NF1 (Neurofibromin 1) is a Protein Coding gene. Diseases associated with NF1 include Neurofibromatosis-Noonan Syndrome and Neurofibromatosis, Type I. Among its related pathways are RET signaling and Signaling by GPCR. Gene Ontology (GO) annotations related to this gene include binding and phosphatidylcholine binding. An important paralog of this gene is RASA3.Stimulates the GTPase activity of Ras. NF1 shows greater affinity for Ras GAP, but lower specific activity. May be a regulator of Ras activity.

Chromosomal Localization:

17q11.2

Expression System:

Baculovirus

Accession Number:

NP_001035957.1

UniProt Accession Number:

P21359

DNA Source:

N/A

Immunogen:

Recombinant Full Length Protein

Vector Name:

xx

Extinction Coefficient:

Buffers:

Expressed Sequence:

MGSGSHHHHHHRSSITSLYKKVGENLYFQGAAHRPVEWVQAVVSRFDEQL
PIKTGQQNTHTKVSTEHNKECLINISKYKFSLVISGLTTILKNVNNMRIF
GEAAEKNLYLSQLIILDTLEKCLAGQPKDTMRLDETMLVKQLLPEICHFL
HTCREGNQHAAELRNSASGVLFSLSCNNFNAVFSRISTRLQELTVCSEDN
VDVHDIELLQYINVDCAKLKRLLKETAFKFKALKKVAQLAVINSLEKAFW
NWVENYPDEFTKLYQIPQTDMAECAEKLFDLVDGFAESTKRKAAVWPLQI
ILLILCPEIIQDISKDVVDENNMNKKLFLDSLRKALAGHGGSRQLTESAA
IACVKLCKASTYINWEDNSVIFLLVQSMVVDLKNLLFNPSKPFSRGSQPA
DVDLMIDCLVSCFRISPHNNQHFKICLAQNSPSTFHYVLVNSLHRIITNS
ALDWWPKIDAVYCHSVELRNMFGETLHKAVQGCGAHPAIRMAPSLTFKEK
VTSLKFKEKPTDLETRSYKYLLLSMVKLIHADPKLLLCNPRKQGPETQGS
TAELITGLVQLVPQSHMPEIAQEAMEALLVLHQLDSIDLWNPDAPVETFW
EISSQMLFYICKKLTSHQMLSSTEILKWLREILICRNKFLLKNKQADRSS
CHFLLFYGVGCDIPSSGNTSQMSMDHEELLRTPGASLRKGKGNSSMDSAA
GCSGTPPICRQAQTKLEVALYMFLWNPDTEAVLVAMSCFRHLCEEADIRC
GVDEVSVHNLLPNYNTFMEFASVSNMMSTGRAALQKRVMALLRRIEHPTA
GNTEAWEDTHAKWEQATKLILNYPKAKMEDGQAAESLHKTIVKRRMSHVS
GGGSIDLSDTDSLQEWINMTGFLCALGGVCLQQRSNSGLATYSPPMGPVS
ERKGSMISVMSSEGNADTPVSKFMDRLLSLMVCNHEKVGLQIRTNVKDLV
GLELSPALYPMLFNKLKNTISKFFDSQGQVLLTDTNTQFVEQTIAIMKNL
LDNHTEGSSEHLGQASIETMMLNLVRYVRVLGNMVHAIQIKTKLCQLVEV
MMARRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAADDDVKCLTRDLDQA
SMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDCSEVE
DESAQTGGRKRGMSRRLASLRHCTVLAMSNLLNANVDSGLMHSIGLGYHK
DLQTRATFMEVLTKILQQGTEFDTLAETVLADRFERLVELVTMMGDQGEL
PIAMALANVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSM
QTLFRGNSLASKIMTFCFKVYGATYLQKLLDPLLRIVITSSDWQHVSFEV
DPTRLEPSESLEENQRNLLQMTEKFFHAIISSSSEFPPQLRSVCHCLYQV
VSQRFPQNSIGAVGSAMFLRFINPAIVSPYEAGILDKKPPPRIERGLKLM
SKILQSIANHVLFTKEEHMRPFNDFVKSNFDAARRFFLDIASDCPTSDAV
NHSLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMATL
LAYLGPPEHKPVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLSI
FYQAGTSKAGNPIFYYVARRFKTGQINGDLLIYHVLLTLKPYYAKPYEIV
VDLTHTGPSNRFKTDFLSKWFVVFPGFAYDNVSAVYIYNCNSWVREYTKY
HERLLTGLKGSKRLVFIDCPGKLAEHIEHEQQKLPAATLALEEDLKVFHN
ALKLAHKDTKVSIKVGSTAVQVTSAERTKVLGQSVFLNDIYYASEIEEIC
LVDENQFTLTIANQGTPLTFMHQECEAIVQSIIHIRTRWELSQPDSIPQH
TKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLCALTCTFNLKIEGQL
LETSGLCIPANNTLFIVSISKTLAANEPHLTLEFLEECISGFSKSSIELK
HLCLEYMTPWLSNLVRFCKHNDDAKRQRVTAILDKLITMTINEKQMYPSI
QAKIWGSLGQITDLLDVVLDSFIKTSATGGLGSIKAEVMADTAVALASGN
VKLVSSKVIGRMCKIIDKTCLSPTPTLEQHLMWDDIAILARYMLMLSFNN
SLDVAAHLPYLFHVVTFLVATGPLSLRASTHGLVINIIHSLCTCSQLHFS
EETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPGSY
ERETFALTSLETVTEALLEIMEACMRDIPTCKWLDQWTELAQRFAFQYNP
SLQPRALVVFGCISKRVSHGQIKQIIRILSKALESCLKGPDTYNSQVLIE
ATVIALTKLQPLLNKDSPLHKALFWVAVAVLQLDEVNLYSAGTALLEQNL
HTLDSLRIFNDKSPEEVFMAIRNPLEWHCKQMDHFVGLNFNSNFNFALVG
HLLKGYRHPSPAIVARTVRILHTLLTLVNKHRNCDKFEVNTQSVAYLAAL
LTVSEEVRSRCSLKHRKSLLLTDISMENVPMDTYPIHHGDPSYRTLKETQ
PWSSPKGSEGYLAATYPTVGQTSPRARKSMSLDMGQPSQANTKKLLGTRK
SFDHLISDTKAPKRQEMESGITTPPKMRRVAETDYEMETQRISSSQQHPH
LRKVSVSESNVLLDEEVLTDPKIQALLLTVLATLVKYTTDEFDQRILYEY
LAEASVVFPKVFPVVHNLLDSKINTLLSLCQDPNLLNPIHGIVQSVVYHE
ESPPQYQTSYLQSFGFNGLWRFAGPFSKQTQIPDYAELIVKFLDALIDTY
LPGIDEETSEESLLTPTSPYPPALQSQLSITANLNLSNSMTSLATSQHSP
GIDKENVELSPTTGHCNSGRTRHGSASQVQKQRSAGSFKRNSIKKIV

Native Sequence:

Calculated Isoelectric Point:

7.02

Molecular Weight:

320316

Last Updated:

06/23/2021

Links

Characterization Data

SOPs

No SOPs available.

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