Mechanistic Target Of Rapamycin Peptide 8


Background

Catalog Number:

CPTC-MTOR-10

Target Antigen:

Mechanistic Target Of Rapamycin Peptide 8

Isotype:

IgG

Species:

Rabbit Recombinant Cloned Antibody

Last Updated:

09/01/2022

Antigen Recognition(s):

Peptide

Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (607.4 KB)

CPTC-MTOR-10 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


  Immunofluorescence - HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (607.4 KB)

CPTC-MTOR-10 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


  Western Blot - Recombinant Protein or Peptide

CPTC-MTOR-10 Western Blot (Recombinant Protein)

Result: Negative

Western blot using CPTC-MTOR-10 as primary antibody against human FK506 binding protein 12-rapamycin associated protein 1 (FRAP1/MTOR) recombinant protein (lane 2). Molecular weight standards are also included (lane 1). Expected molecular weight – 288.7 kDa.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-MTOR-10 as primary antibody against cell lysates A549 (lane 2), HeLa (lane 3), Jurkat (lane 4), MCF-7 (lane 5), MOLT-4 (lane 6), and U87 (lane 7). Molecular weight standards are also included (lane 1). U87 is presumed positive. All other cells lines are negative. Expected molecular weight – 289 kDa. Click image to enlarge

CPTC-MTOR-10 Western Blot Cell Lysates

Result: Presumed Positive (with additional bands)

Western Blot using CPTC-MTOR-10 as primary antibody against cell lysates A549 (lane 2), HeLa (lane 3), Jurkat (lane 4), MCF-7 (lane 5), MOLT-4 (lane 6), and U87 (lane 7). Molecular weight standards are also included (lane 1). U87 is presumed positive. All other cells lines are negative. Expected molecular weight – 289 kDa.


Characterization SOP Files

Background

Catalog Number:

CPTC-MTOR-9

RRID:

AB_2820270

Target Antigen:

Mechanistic Target Of Rapamycin Peptide 8

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

09/01/2022

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous, Phosphorylation

Thematic Panel(s):

Ras Pathway

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Publications
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (346.7 KB)

CPTC-MTOR-9 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


  Immunofluorescence

CPTC-MTOR-9 Immunofluorescence

Result: Negative

Immunofluorescence staining of MCF10a cells stably transfected with KRAS (MCF10a-KRAS) using CPTC-MTOR-9 antibody. MTOR protein expression was not detected.


  Immunofluorescence - HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (346.7 KB)

CPTC-MTOR-9 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-MTOR-9 as primary Ab against recombinant MTOR (lane 2). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-MTOR-9 Western Blot

Result: Positive

Western Blot using CPTC-MTOR-9 as primary Ab against recombinant MTOR (lane 2). Also included are molecular wt. standards (lane 1).


  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-MTOR-9 as primary Ab against MCF10A-KRAS cell lysate (lane 2). Also included are molecular wt. standards (lane 1). Click image to enlarge

CPTC-MTOR-9 Cell Lysate Western Blot

Result: Positive

Western Blot using CPTC-MTOR-9 as primary Ab against MCF10A-KRAS cell lysate (lane 2). Also included are molecular wt. standards (lane 1).


Background

NCI Identification Number:

00256

Antigen Name:

Mechanistic Target Of Rapamycin Peptide 8

CPTC Name:

CPTC-MTOR Peptide 8

Aliases:

Mechanistic Target Of Rapamycin; Mechanistic Target Of Rapamycin (Serine/Threonine Kinase); Rapamycin And FKBP12 Target 1; Mammalian Target Of Rapamycin; FK506-Binding Protein 12-Rapamycin Complex-Associated Protein 1; FK506 Binding Protein 12-Rapamycin Associated Protein 2; FKBP12-Rapamycin Complex-Associated Protein 1; Rapamycin Associated Protein FRAP2; FKBP-Rapamycin Associated Protein; Rapamycin Target Protein 1; FRAP1; FRAP2; RAFT1; RAPT1; FRAP; DJ576K7.1 (FK506 Binding Protein 12-Rapamycin Associated Protein 1); FK506 Binding Protein 12-Rapamycin Associated Protein 1; FKBP12-Rapamycin Complex-Associated Protein; Rapamycin Target Protein; EC 2.7.11.1; MTOR; SKS

Function:

The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.

MTOR (Mechanistic Target Of Rapamycin) is a Protein Coding gene. Diseases associated with MTOR include smith-kingsmore syndrome and isolated focal cortical dysplasia type iib. Among its related pathways are Gene Expression and Signaling by GPCR. GO annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein serine/threonine kinase activity. An important paralog of this gene is SMG1.

Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals. MTOR directly or indirectly regulates the phosphorylation of at least 800 proteins. Functions as part of 2 structurally and functionally distinct signaling complexes mTORC1 and mTORC2 (mTOR complex 1 and 2). Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. This includes phosphorylation of EIF4EBP1 and release of its inhibition toward the elongation initiation factor 4E (eiF4E). Moreover, phosphorylates and activates RPS6KB1 and RPS6KB2 that promote protein synthesis by modulating the activity of their downstream targets including ribosomal protein S6, eukaryotic translation initiation factor EIF4B, and the inhibitor of translation initiation PDCD4. Stimulates the pyrimidine biosynthesis pathway, both by acute regulation through RPS6KB1-mediated phosphorylation of the biosynthetic enzyme CAD, and delayed regulation, through transcriptional enhancement of the pentose phosphate pathway which produces 5-phosphoribosyl-1-pyrophosphate (PRPP), an allosteric activator of CAD at a later step in synthesis, this function is dependent on the mTORC1 complex. Regulates ribosome synthesis by activating RNA polymerase III-dependent transcription through phosphorylation and inhibition of MAF1 an RNA polymerase III-repressor. In parallel to protein synthesis, also regulates lipid synthesis through SREBF1/SREBP1 and LPIN1. To maintain energy homeostasis mTORC1 may also regulate mitochondrial biogenesis through regulation of PPARGC1A. mTORC1 also negatively regulates autophagy through phosphorylation of ULK1. Under nutrient sufficiency, phosphorylates ULK1 at Ser-758, disrupting the interaction with AMPK and preventing activation of ULK1. Also prevents autophagy through phosphorylation of the autophagy inhibitor DAP. mTORC1 exerts a feedback control on upstream growth factor signaling that includes phosphorylation and activation of GRB10 a INSR-dependent signaling suppressor. Among other potential targets mTORC1 may phosphorylate CLIP1 and regulate microtubules. As part of the mTORC2 complex MTOR may regulate other cellular processes including survival and organization of the cytoskeleton. Plays a critical role in the phosphorylation at Ser-473 of AKT1, a pro-survival effector of phosphoinositide 3-kinase, facilitating its activation by PDK1. mTORC2 may regulate the actin cytoskeleton, through phosphorylation of PRKCA, PXN and activation of the Rho-type guanine nucleotide exchange factors RHOA and RAC1A or RAC1B. mTORC2 also regulates the phosphorylation of SGK1 at Ser-422. Regulates osteoclastogensis by adjusting the expression of CEBPB isoforms (By similarity).

Chromosomal Localization:

1p36

Accession Number:

NP_004949.1

UniProt Accession Number:

P42345

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

TGTTVPESIH(pS)FIGDGLVKPEALNKK

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

2860

Last Updated:

04/19/2018

Links

Characterization Data

SOPs

No SOPs available.

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