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Lymphocyte Antigen 6 Family Member D Peptide 1

Antibody: CPTC-Ly6D-4
Background

Catalog Number:

CPTC-Ly6D-4

Target Antigen:

Lymphocyte Antigen 6 Family Member D Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

08/25/2023

Antigen Recognition(s):

Peptide, Recombinant Full-length

Characterization Data [Compare Characterization Data]
  Affinity Measurement by Biolayer Interferometry
Click to enlarge image Affinity and binding kinetics of CPTC-LY6D-4 antibody and full-length LY6D recombinant protein were measured using bio-layer interferometry. LY6D recombinant protein was amine coupled onto AR2G biosensors. CPTC-LY6D-4 antibody was used as analyte and was titrated at 256 nM, 64 nM, 16 nM, 4 nM, 1.0 nM and 0.25 nM. Buffer only and biosensors immobilized without recombinant protein were used as references for background subtraction. All binding data was double referenced and analyzed globally using a 1:2 bivalent fitting model. Click image to enlarge

CPTC-LY6D-4 Affinity and Kinetics (Bio-Layer Interferometry) 

Result: High Binding

Affinity and binding kinetics of CPTC-LY6D-4 antibody and full-length LY6D recombinant protein were measured using bio-layer interferometry. LY6D recombinant protein was amine coupled onto AR2G biosensors. CPTC-LY6D-4 antibody was used as analyte and was titrated at 256 nM, 64 nM, 16 nM, 4 nM, 1.0 nM and 0.25 nM. Buffer only and biosensors immobilized without recombinant protein were used as references for background subtraction. All binding data was double referenced and analyzed globally using a 1:2 bivalent fitting model.


Characterization SOP Files

  Affinity Measurement by SPR
Click to enlarge image Affinity and binding kinetics of CPTC-LY6D-4 and full length LY6D recombinant protein were measured using surface plasmon resonance. LY6D recombinant protein was amine coupled onto a Series S CM5 biosensor chip. CPTC-LY6D-4 antibody was used as analyte and was titrated at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, and 1 nM. All binding data were double-referenced and analyzed globally using a 1:2 bivalent fitting model. Click image to enlarge

CPTC-LY6D-4 Affinity and Kinetics (Surface Plasmon Resonance)

Result: High Binding

Affinity and binding kinetics of CPTC-LY6D-4 and full length LY6D recombinant protein were measured using surface plasmon resonance. LY6D recombinant protein was amine coupled onto a Series S CM5 biosensor chip. CPTC-LY6D-4 antibody was used as analyte and was titrated at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, and 1 nM. All binding data were double-referenced and analyzed globally using a 1:2 bivalent fitting model.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using as CPTC-LY6D-4 rabbit antibody against full length LY6D recombinant protein. Recombinant protein was coated on the plate and CPTC-LY6D-4 antibody was titrated and detected using the goat anti-rabbit antibody and TMB. Click image to enlarge

CPTC-LY6D-4 Indirect ELISA

Result: High Binding

Indirect ELISA using as CPTC-LY6D-4 rabbit antibody against full length LY6D recombinant protein. Recombinant protein was coated on the plate and CPTC-LY6D-4 antibody was titrated and detected using the goat anti-rabbit antibody and TMB.


Characterization SOP Files

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Antigen: Lymphocyte Antigen 6 Family Member D Peptide 1
Background

NCI Identification Number:

00542

Antigen Name:

Lymphocyte Antigen 6 Family Member D Peptide 1

CPTC Name:

CPTC-Ly6D Peptide 1

Aliases:

Lymphocyte Antigen 6 Family Member D; Lymphocyte Antigen 6 Complex, Locus D; E48 Antigen; Ly-6D; E48; Lymphocyte Antigen 6D

Function:

LY6D (Lymphocyte Antigen 6 Family Member D) is a Protein Coding gene. Diseases associated with LY6D include Squamous Cell Carcinoma, Head And Neck. Among its related pathways are Metabolism of proteins and B Cell Development Pathways.May act as a specification marker at earliest stage specification of lymphocytes between B- and T-cell development. Marks the earliest stage of B-cell specification.

Chromosomal Localization:

8q24.3

Accession Number:

NP_003686.1

UniProt Accession Number:

Q14210

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

NTVEPLRGNLVKKDC and CTPSYTLQGQVSSGTSSTQ

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1650

Last Updated:

03/06/2023

Links

Characterization Data

SOPs

No SOPs available.

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