Background
Catalog Number:
CPTC-GPC6-1
Target Antigen:
Glypican 6
Isotype:
IgG
Species:
Rabbit Monoclonal Antibody
Last Updated:
05/23/2025
Antigen Recognition(s):
Recombinant Full-length, Endogenous
Characterization Data [Compare Characterization Data]
Affinity Measurement by Biolayer Interferometry
CPTC-GPC6-1 Affinity and Kinetics (Bio-Layer Interferometry)
Result: No Binding
Affinity and binding kinetics of CPTC-GPC6-1 antibody and full-length GPC6 recombinant protein were measured using biolayer interferometry. GPC6 full length recombinant protein was amine coupled onto AR2G biosensors. CPTC-GPC6-1 antibody was used as analyte and was titrated at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM, and 0.25 nM. Buffer only and biosensors having no recombinant protein were used as references for background subtraction. All binding data were analyzed globally.
IP
CPTC-GPC6-1 Immunoprecipitation Simple Western Blot (Cell lysates)
Result: Positive
Immunoprecipitation of GPC in cancer cell lysates using CPTC-GPC6-1 as capture antibody. Eluates were tested in SW, using CPTC-GPC-1 as detection antibody. The antibody was able to pull down the target in cell lysates HeLa, HepG2, SF-539, SNB-75 and SW620, but not in HL-60 cell lysate (top panel). IP results were compared with cell lysates direct analysis in SW, using CPTC-GPC-1 as detection antibody (bottom panel).
Western Blot - Over-expressed transient protein in cell lysate
CPTC-GPC6-1 Simple Western Blot (Overexpressed Cell Lysate)
Result: Presumed Positive (with additional bands)
Simple western using CPTC-GPC6-1 as detection antibody against the over-expressed lysate of human GPC6. The antibody presumably recognizes the target protein.
Characterization SOP Files
Background
NCI Identification Number:
00540
Antigen Name:
Glypican 6
CPTC Name:
CPTC-GPC6
Aliases:
Glypican 6; Glypican Proteoglycan 6; Glypican-6; OMIMD1
Function:
The glypicans comprise a family of glycosylphosphatidylinositol-anchored heparan sulfate proteoglycans, and they have been implicated in the control of cell growth and cell division. The glypican encoded by this gene is a putative cell surface coreceptor for growth factors, extracellular matrix proteins, proteases and anti-proteases. Mutations in this gene are associated with omodysplasia 1.GPC6 (Glypican 6) is a Protein Coding gene. Diseases associated with GPC6 include Omodysplasia 1 and Omodysplasia. Among its related pathways are Apoptotic Pathways in Synovial Fibroblasts and GPCR Pathway. Gene Ontology (GO) annotations related to this gene include heparan sulfate proteoglycan binding. An important paralog of this gene is GPC4.Cell surface proteoglycan that bears heparan sulfate. Putative cell surface coreceptor for growth factors, extracellular matrix proteins, proteases and anti-proteases (By similarity). Enhances migration and invasion of cancer cells through WNT5A signaling.
Chromosomal Localization:
13q31.3-q32.1
Expression System:
Mammalian
Accession Number:
NP_005699.1
UniProt Accession Number:
Q9Y625
DNA Source:
N/A
Immunogen:
Recombinant Full Length Protein
Vector Name:
N/A
Extinction Coefficient:
Buffers:
Expressed Sequence:
etgDVKARSCGEVRQAYGAKGFSLADIPYQEIAGEHLRICPQEYTCCTTE
MEDKLSQQSKLEFENLVEETSHFVRTTFVSRHKKFDEFFRELLENAEKSL
NDMFVRTYGMLYMQNSEVFQDLFTELKRYYTGGNVNLEEMLNDFWARLLE
RMFQLINPQYHFSEDYLECVSKYTDQLKPFGDVPRKLKIQVTRAFIAART
FVQGLTVGREVANRVSKVSPTPGCIRALMKMLYCPYCRGLPTVRPCNNYC
LNVMKGCLANQADLDTEWNLFIDAMLLVAERLEGPFNIESVMDPIDVKIS
EAIMNMQENSMQVSAKVFQGCGQPKPAPALRSARSAPENFNTRFRPYNPE
ERPTTAAGTSLDRLVTDIKEKLKLSKKVWSALPYTICKDESVTAGTSNEE
ECWNGHSKARYLPEIMNDGLTNQINNPEVDVDITRPDTFIRQQIMALRVM
TNKLKNAYNGNDVNFQDTgtlevlfq
Native Sequence:
Calculated Isoelectric Point:
Molecular Weight:
54000
Last Updated:
01/19/2023
Links
- EnsEMBL - EnsEMBL GPC6
- Entrez - Entrez GPC6
- Uniprot - Uniprot Q9Y625
Characterization Data
SOPs
No SOPs available.
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