PRINT

Family With Sequence Similarity 53 Member C Peptide 1

Antibody: CPTC-FAM53C-1
Background

Catalog Number:

CPTC-FAM53C-1

Target Antigen:

Family With Sequence Similarity 53 Member C Peptide 1

Isotype:

IgG

Species:

Rabbit Recombinant Cloned Antibody

Last Updated:

03/25/2025

Antigen Recognition(s):

Peptide

Characterization Data [Compare Characterization Data]
  Affinity Measurement by Biolayer Interferometry
Click to enlarge image Affinity and binding kinetics of CPTC-FAM53C-1 and BSA conjugated peptide, CAPSKLW-pT-PIKH, were measured using biolayer interferometry. BSA-conjugated peptide was amine coupled to the AR2G biosensors. The CPTC-FAM53C-1 antibody at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1.0 nM and 0.25 nM, was used as analyte. Buffer only and biosensors immobilized without peptide were used as references for background subtraction. All binding data were analyzed globally using a 1:2 (bivalent) fitting model. Click image to enlarge

CPTC-FAM53C-1 Affinity and Kinetics (Bio-Layer Interferometry) 

Result: High Binding

Affinity and binding kinetics of CPTC-FAM53C-1 and BSA conjugated peptide, CAPSKLW-pT-PIKH, were measured using biolayer interferometry. BSA-conjugated peptide was amine coupled to the AR2G biosensors. The CPTC-FAM53C-1 antibody at 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1.0 nM and 0.25 nM, was used as analyte. Buffer only and biosensors immobilized without peptide were used as references for background subtraction. All binding data were analyzed globally using a 1:2 (bivalent) fitting model.


Characterization SOP Files

  Affinity Measurement by SPR
Click to enlarge image Affinity and binding kinetics of CPTC-FAM53C-1 and BSA-conjugated peptide, CAPSKLW-pT-PIKH, were measured using surface plasmon resonance. BSA conjugated peptide was amine coupled onto a Series S CM5 biosensor chip. CPTC-FAM53C-1 antibody at, 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM, and 0.25 nM, was used as analyte. Binding data were double-referenced and analyzed globally using a bivalent fitting model. Click image to enlarge

CPTC-FAM53C-1 Affinity and Kinetics (Surface Plasmon Resonance)

Result: High Binding

Affinity and binding kinetics of CPTC-FAM53C-1 and BSA-conjugated peptide, CAPSKLW-pT-PIKH, were measured using surface plasmon resonance. BSA conjugated peptide was amine coupled onto a Series S CM5 biosensor chip. CPTC-FAM53C-1 antibody at, 1024 nM, 256 nM, 64 nM, 16 nM, 4 nM, 1 nM, and 0.25 nM, was used as analyte. Binding data were double-referenced and analyzed globally using a bivalent fitting model.


Characterization SOP Files

  Flow Cytometry

CPTC-FAM53C-1 Flow Cytometry

Result: Negative

Flow cytometric analysis of thyroid medullary TT cells, asynchronous (blue) or synchronous (serum starved 24 hours, green) using CPTC-FAM53C-1 rabbit antibody (solid lines) or concentration matched rabbit isotype control antibody (dashed lines). TT cells were permeabilized, fixed, and then stained with either CPTC-FAM53C-1 or rabbit isotype control antibody. A BV421 conjugated goat anti-rabbit IgG (H+L) was used as a secondary antibody. All data were analyzed using FlowJo.


Characterization SOP Files

  IHC Tissue
Click to enlarge image Tissue Micro-Array (TMA) core of breast cancer showing cytoplasmic and nuclear staining using Antibody CPTC-FAM53C-1. Titer: 1:50 The pattern of staining is correct but lacks sufficient literature data to confirm cell type specificity. Click image to enlarge

CPTC-FAM53C-1 IHC Tissue

Result: Uncertain

Tissue Micro-Array (TMA) core of breast cancer showing cytoplasmic and nuclear staining using Antibody CPTC-FAM53C-1. Titer: 1:50
The pattern of staining is correct but lacks sufficient literature data to confirm cell type specificity.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-FAM53C-1 as primary antibody against BSA-conjugated peptide, CAPSKLW-pT-PIKH, coated on the plate and detected using the goat anti-rabbit antibody and TMB. Click image to enlarge

CPTC-FAM53C-1 Indirect ELISA

Result: High Binding

Indirect ELISA using CPTC-FAM53C-1 as primary antibody against BSA-conjugated peptide, CAPSKLW-pT-PIKH, coated on the plate and detected using the goat anti-rabbit antibody and TMB.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-FAM53C-1 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Over-expressed transient protein in cell lysate
Click to enlarge image Western Blot using CPTC-FAM53C-1 as primary antibody against the over-expressed lysate of FAM53C. The antibody is presumedbly able to recognize the target protein in the tested lysate (pink signal). The same membrane was probed with an anti CytC antibody and the housekeeping protein was detected (green signal) Click image to enlarge

CPTC-FAM53C-1 Western Blot (Over expressed Cell Lysates)

Result: Presumed Positive (with additional bands)

Western Blot using CPTC-FAM53C-1 as primary antibody against the over-expressed lysate of FAM53C. The antibody is presumedbly able to recognize the target protein in the tested lysate (pink signal). The same membrane was probed with an anti CytC antibody and the housekeeping protein was detected (green signal)


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-FAM53C-1 Western Blot (Cell Lysate)

Result: Negative

Western blot using CPTC-FAM53C-1 as primary antibody against the cell lysates of TT, H146 and H1184 cells, asynchronous (+) or synchronous - serum starved 24hours (-). The antibody was not able to detect the target protein in any of the tested cell lysates. The same membrane was probed with an anti CytC antibody, and all the cell lysates show the presence of the housekeeping protein.


Characterization SOP Files

[top]

Antigen: Family With Sequence Similarity 53 Member C Peptide 1
Background

NCI Identification Number:

00539

Antigen Name:

Family With Sequence Similarity 53 Member C Peptide 1

CPTC Name:

CPTC-FAM53C Peptide 1

Aliases:

Family With Sequence Similarity 53 Member C; C5orf6; Protein FAM53C; Family With Sequence Similarity 53, Member C; Chromosome 5 Open Reading Frame 6; Putative Nuclear Protein

Function:

The protein encoded by this gene belongs to the FAM53 protein family. FAM53 protein family members bind to a transcriptional regulator that modulates cell proliferation. Alternative splicing results in multiple transcript variants.FAM53C (Family With Sequence Similarity 53 Member C) is a Protein Coding gene. Diseases associated with FAM53C include Childhood Acute Megakaryoblastic Leukemia. An important paralog of this gene is FAM53A.

Chromosomal Localization:

5q31.2

Accession Number:

UniProt Accession Number:

Q9NYF3

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

CAPSKLW-pT-PIKH

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1320

Last Updated:

11/18/2022

Links

Characterization Data

SOPs

No SOPs available.

[top]

Don't have Adobe Reader™?

Get it for free at Adobe.com

  [top]