Zinc Finger E-Box Binding Homeobox 2 Peptide 1


Background

Catalog Number:

CPTC-ZEB2-1

Target Antigen:

Zinc Finger E-Box Binding Homeobox 2 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

02/05/2021

Antigen Recognition(s):

Peptide, Recombinant Full-length

Thematic Panel(s):

Epithelial Mesenchymal Transition (EMT)

SOP:

Publications
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (365.8 KB)

CPTC-ZEB2-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


  IPMS
Download Immuno-precipitation data obtained incubating CPTC-ZEB2-1 and the recombinant domain of ZEB2 (aa647-1214, expected, MW ~64KDa) (142.7 KB)

CPTC-ZEB2-1 IPMS

Result: Negative

Immuno-precipitation data obtained incubating CPTC-ZEB2-1 and the recombinant domain of ZEB2 (aa647-1214, expected, MW ~64KDa)


  Western Blot - Cell Lysate
Click to enlarge image Western Blot using CPTC-ZEB2-1 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 136 KDa. Positive for A549, Hela, Jurkat and weak for H226 and MCF7.  Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-ZEB2-1 Endogenous Western Blot

Result: Positive

Western Blot using CPTC-ZEB2-1 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 136 KDa. Positive for A549, Hela, Jurkat and weak for H226 and MCF7. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Cell Lysate
Click to enlarge image Automated Western Blot using CPTC-ZEB2-1 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 136 KDa. All cell lysates negative.  Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-ZEB2-1 Endogenous Automated Western Blot

Result: Negative

Automated Western Blot using CPTC-ZEB2-1 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 136 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1).


  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-ZEB2-1 as primary antibody against recombinant ZEB2 protein domain comprising amino acids 647-1214 (lane 2) with expected MW of 58.1 KDa. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-ZEB2-1 Western Blot (Recombinant Protein)

Result: Positive

Western Blot using CPTC-ZEB2-1 as primary antibody against recombinant ZEB2 protein domain comprising amino acids 647-1214 (lane 2) with expected MW of 58.1 KDa. Molecular weight standards are also included (lane 1).


Characterization SOP Files

Background

NCI Identification Number:

00074

Antigen Name:

Zinc Finger E-Box Binding Homeobox 2 Peptide 1

CPTC Name:

CPTC-ZEB2 Peptide 1

Keywords:

Zinc Finger E-Box Binding Homeobox 2; ZFHX1B; SIP1; SMADIP1; Zinc Finger Homeobox 1b; SMAD Interacting Protein 1; HSPC082; SIP-1; KIAA0569; ZFX1B; Zinc Finger Homeobox Protein 1b

Function:

This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.

ZEB1 (zinc finger E-box binding homeobox 1) is a protein-coding gene. Diseases associated with ZEB1 include corneal dystrophy, posterior polymorphous, 3, and corneal dystrophy, fuchs endothelial, 6. GO annotations related to this gene include transcription coactivator activity and sequence-specific DNA binding transcription factor activity. An important paralog of this gene is ZEB2.

Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). Promotes tumorigenicity by repressing stemness-inhibiting microRNAs.

Chromosomal Localization:

2q22.3

NCBI Accession Number:

NP_001165124

UniProt Accession Number:

O60315

DNA Source:

The NCI acknowledges the generous advice and gene clones provided by Robert A. Weinberg at the Ludwig Center for Cancer Research of the Massachusetts Institute of Technology. These reagents have been provided by the Division of Cancer Treatment and Diagnosis (DCTD) of the National Cancer Institute (NCI) using funding of the American Recovery and Reinvestment Act of 2009

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

cEELQAEPLDLSLPKQMKEP

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

2090

Last Updated:

09/16/2019

Links

Characterization Data

SOPs

No SOPs available.

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