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Zinc Finger E-Box Binding Homeobox 1 Peptide 1

Antibody: CPTC-ZEB1-1
Background

Catalog Number:

CPTC-ZEB1-1

RRID:

AB_2827861

Target Antigen:

Zinc Finger E-Box Binding Homeobox 1 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

02/09/2024

Antigen Recognition(s):

Peptide

Thematic Panel(s):

Epithelial Mesenchymal Transition (EMT)

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Publications
Characterization Data [Compare Characterization Data]
  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (312.6 KB)

CPTC-ZEB1-1 Evaluation by the Human Protein Atlas

Result: Negative

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-ZEB1-1 as primary antibody against ZEB1 domain comprising amino acids 300-510. Click image to enlarge

CPTC-ZEB1-1 Indirect ELISA

Result: Positive

Indirect ELISA using CPTC-ZEB1-1 as primary antibody against ZEB1 domain comprising amino acids 300-510.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide

CPTC-ZEB1-1 recombinant protein WB

Result: Negative

Western Blot using CPTC-ZEB1-1 as primary antibody against recombinant ZEB1 protein (lane 2) with expected MW of 124 KDa.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-ZEB1-1 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 124 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-ZEB1-1 Endogenous Western Blot

Result: Negative

Western Blot using CPTC-ZEB1-1 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 124 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated Western Blot using CPTC-ZEB1-1 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 124 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-ZEB1-1 Endogenous Automated Western Blot

Result: Negative

Automated Western Blot using CPTC-ZEB1-1 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 124 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1).


Characterization SOP Files

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Antibody: CPTC-ZEB1-2
Background

Catalog Number:

CPTC-ZEB1-2

RRID:

AB_2827862

Target Antigen:

Zinc Finger E-Box Binding Homeobox 1 Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

05/28/2024

Antigen Recognition(s):

Peptide, Endogenous

Thematic Panel(s):

Epithelial Mesenchymal Transition (EMT)

Purchase
Publications
Characterization Data [Compare Characterization Data]
  CyTOF
Click to enlarge image Imaging mass cytometry on normal kidney tissue core using CPTC-ZEB1-2 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (colon, pancreas, breast, testis, endometrium, appendix, and kidney) and cancer tissues (breast, colon, ovarian, and lung). Click image to enlarge

CPTC-ZEB1-2 Immuno-Mass Cytometry

Result: Positive

Imaging mass cytometry on normal kidney tissue core using CPTC-ZEB1-2 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (colon, pancreas, breast, testis, endometrium, appendix, and kidney) and cancer tissues (breast, colon, ovarian, and lung).


Characterization SOP Files

  IHC HPA
Download This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org) (488.4 KB)

CPTC-ZEB1-2 Evaluation by the Human Protein Atlas

Result: Positive

This PDF contains the evaluation results provided by the Human Protein Atlas (www.proteinatlas.org)


Characterization SOP Files

  Immunofluorescence - HPA
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli. Human assay: A549 fixed with PFA, dilution: 1:50 Human assay: RH-30 fixed with PFA, dilution: 1:50 Human assay: U-2 OS fixed with PFA, dilution: 1:50 Click image to enlarge

CPTC-ZEB1-2 Evaluation by the Human Protein Atlas

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is supported by literature. Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & nucleoli.

Human assay: A549 fixed with PFA, dilution: 1:50
Human assay: RH-30 fixed with PFA, dilution: 1:50
Human assay: U-2 OS fixed with PFA, dilution: 1:50


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA using CPTC-ZEB1-2 as primary antibody against ZEB1 domain comprising amino acids 300-510. Click image to enlarge

CPTC-ZEB1-2 Indirect ELISA

Result: Positive

Indirect ELISA using CPTC-ZEB1-2 as primary antibody against ZEB1 domain comprising amino acids 300-510.


Characterization SOP Files

  Western Blot - HPA - tissue or cell lysate
Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). Band of predicted size in kDa (+/-20%) with additional bands present. Analysis performed using a standard panel of samples. Antibody dilution: 1:500. Click image to enlarge

CPTC-ZEB1-2 Evaluation by the Human Protein Atlas

Result: Presumed Positive (with additional bands)

Results provided by the Human Protein Atlas (www.proteinatlas.org). Band of predicted size in kDa (+/-20%) with additional bands present. Analysis performed using a standard panel of samples. Antibody dilution: 1:500.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide

CPTC-ZEB1-2 recombinant protein WB

Result: Negative

Western Blot using CPTC-ZEB1-2 as primary antibody against recombinant ZEB1 protein (lane 2) with expected MW of 124 KDa.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-ZEB1-2 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 124 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-ZEB1-2 Endogenous Western Blot

Result: Negative

Western Blot using CPTC-ZEB1-2 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 124 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1).


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Automated Western Blot using CPTC-ZEB1-2 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 124 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1). Click image to enlarge

CPTC-ZEB1-2 Endogenous Automated Western Blot

Result: Negative

Automated Western Blot using CPTC-ZEB1-2 as primary antibody against cell lysates A549, H226, HeLa, Jurkat and MCF7. Expected MW of 124 KDa. All cell lysates negative. Molecular weight standards are also included (lane 1).


Characterization SOP Files

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Antigen: Zinc Finger E-Box Binding Homeobox 1 Peptide 1
Background

NCI Identification Number:

00072

Antigen Name:

Zinc Finger E-Box Binding Homeobox 1 Peptide 1

CPTC Name:

CPTC-ZEB1 Peptide 1

Aliases:

TCF8; PPCD3; Posterior Polymorphous Corneal Dystrophy 3; Transcription Factor 8 (Represses Interleukin 2 Expression); Negative Regulator Of IL2; AREB6; FECD6; NIL2A; BZP; DELTAEF1; ZFHEP; ZFHX1A; Delta-Crystallin Enhancer Binding Factor 1; Zinc Finger E-Box-Binding Homeobox 1; Zinc Finger Homeodomain Enhancer-Binding Protein; TCF-8; NIL-2-A Zinc Finger Protein; Transcription Factor 8

Function:

Chromosomal Localization:

10p11.22

Accession Number:

NP_001167564.1

UniProt Accession Number:

P37275

DNA Source:

The NCI acknowledges the generous advice and gene clones provided by Robert A. Weinberg at the Ludwig Center for Cancer Research of the Massachusetts Institute of Technology. These reagents have been provided by the Division of Cancer Treatment and Diagnosis (DCTD) of the National Cancer Institute (NCI) using funding of the American Recovery and Reinvestment Act of 2009

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

cIINYSLEQPSQLQVVPQNLK

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

2310

Last Updated:

09/16/2019

Links

Characterization Data

SOPs

No SOPs available.

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