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WAP Four-Disulfide Core Domain 2 Peptide 2

Antibody: CPTC-WFDC2-2
Background

Catalog Number:

CPTC-WFDC2-2

Target Antigen:

WAP Four-Disulfide Core Domain 2 Peptide 2

Isotype:

IgG

Species:

Rabbit Recombinant Cloned Antibody

Last Updated:

02/09/2024

Antigen Recognition(s):

Peptide, Recombinant Full-length, Endogenous

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Characterization Data [Compare Characterization Data]
  IHC Tissue

CPTC-WFDC2-2 IHC Tissue

Result: Negative

This antibody is not suitable for use in an Immunohistochemistry format as described in SOP M-106.


Characterization SOP Files

  IP

CPTC-WFDC2-2 IP-WB

Result: Negative

Antibody CPTC-WFDC2-2 was tested in IP-WB in Cell lines OVCAR-8 and OVCAR-3. The antibody is not suitable for this application.


Characterization SOP Files

  Immuno-MRM
Click to enlarge image iMRM screening of CPTC-WFDC2- against synthetic peptide DQC[+57]QVDSQC[+57]PGQMK (WAP Four-Disulfide Core Domain 2 Peptide 2) Data provided by the Carr Lab, Broad Institute https://www.broadinstitute.org/proteomics/protocols Click image to enlarge

CPTC-WFDC2-2 iMRM screening (synthetic peptide)

Result: Positive

iMRM screening of CPTC-WFDC2- against synthetic peptide DQC[+57]QVDSQC[+57]PGQMK (WAP Four-Disulfide Core Domain 2 Peptide 2)

Data provided by the Carr Lab, Broad Institute
https://www.broadinstitute.org/proteomics/protocols


Characterization SOP Files

  Immunofluorescence
Click to enlarge image Immunofluorescence staining of human cell line OVCAR-3 using CPTC-WFDC2-2 as primary antibody. WFDC2 protein expression shows localization in the cytosol (green). Click image to enlarge

CPTC-WFDC2-2 Immunofluorescence

Result: Positive

Immunofluorescence staining of human cell line OVCAR-3 using CPTC-WFDC2-2 as primary antibody. WFDC2 protein expression shows localization in the cytosol (green).


Characterization SOP Files

  Indirect ELISA

CPTC-WFDC2-2 Indirect ELISA

Result: Negative

Indirect ELISA using CPTC-WFDC2-2 as primary antibody against WFDC2 recombinant protein.


Characterization SOP Files

  NCI 60 Protein Array

CPTC-WFDC2-2 NCI 60 Protein Array

Result: Negative

This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105.


Characterization SOP Files

  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western blot using CPTC-WFDC2-2 as primary antibody against WFDC2 recombinant protein. Molecular weight standards are also included. Click image to enlarge

CPTC-WFDC2-2 Western Blot (Recombinant Protein)

Result: Positive

Western blot using CPTC-WFDC2-2 as primary antibody against WFDC2 recombinant protein. Molecular weight standards are also included.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western blot using CPTC-WFDC2-2 as primary antibody against cell lysates and supernatants of cell lines OVCAR-3, OVCAR-4, OVAR-8 and SK-OV-3. Expected molecular weight is 12 KDa. Molecular weight standards are also included. Click image to enlarge

CPTC-WFDC2-2 Western Blot (Cell Lysates)

Result: Presumed Positive (with additional bands)

Western blot using CPTC-WFDC2-2 as primary antibody against cell lysates and supernatants of cell lines OVCAR-3, OVCAR-4, OVAR-8 and SK-OV-3. Expected molecular weight is 12 KDa. Molecular weight standards are also included.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate
Click to enlarge image Western Blot using CPTC-WFDC2-2 as primary antibody against cell lysates A549, HT-29, MBA-MB-231. Molecular weight standards are also included. Click image to enlarge

CPTC-WFDC2-2 Cell Lysate Western Blot

Result: Negative

Western Blot using CPTC-WFDC2-2 as primary antibody against cell lysates A549, HT-29, MBA-MB-231. Molecular weight standards are also included.


Characterization SOP Files

  Western Blot - Tissue or Cell Lysate

CPTC-WFDC2-2 Single Cell Western Blot (Cell Lysate)

Result: Negative

Single cell western blot using CPTC-WFDC2-2 as a primary antibody against cell lysates. WFDC2 protein was not detected and therefore no expression graphs were generated.


Characterization SOP Files

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Antigen: WAP Four-Disulfide Core Domain 2 Peptide 2
Background

NCI Identification Number:

00326

Antigen Name:

WAP Four-Disulfide Core Domain 2 Peptide 2

CPTC Name:

CPTC-WFDC2 Peptide 2

Aliases:

WAP Four-Disulfide Core Domain 2; WAP5; HE4; WAP Four-Disulfide Core Domain Protein 2; Major Epididymis-Specific Protein E4; Putative Protease Inhibitor WAP5; Epididymal Secretory Protein E4; Epididymal Protein 4; DJ461P17.6; EDDM4; Epididymis-Specific, Whey-Acidic Protein Type, Four-Disulfide Core; Epididymis Secretory Sperm Binding Protein; WAP Domain Containing Protein HE4-V4; WFDC2

Function:

This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation. WFDC2 (WAP Four-Disulfide Core Domain 2) is a Protein Coding gene. Diseases associated with WFDC2 include Fallopian Tube Carcinoma and Ovarian Cancer. Gene Ontology (GO) annotations related to this gene include serine-type endopeptidase inhibitor activity and cysteine-type endopeptidase inhibitor activity.Broad range protease inhibitor.

Chromosomal Localization:

20q13.12

Accession Number:

NP_006094.3

UniProt Accession Number:

Q14508

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

DQCQVDSQCPGQMK

Native Sequence:

Calculated Isoelectric Point:

Molecular Weight:

1540

Last Updated:

08/21/2020

Links

Characterization Data

SOPs

No SOPs available.

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