Ubiquitin Conjugating Enzyme E2I Peptide 1


Background

Catalog Number:

CPTC-UBE2I-1

Target Antigen:

Ubiquitin Conjugating Enzyme E2I Peptide 1

Isotype:

IgG

Species:

Rabbit Monoclonal Antibody

Last Updated:

10/22/2019

Antigen Recognition(s):

Thematic Panel(s):

DNA Damage Response (DDR) Pathway

SOP:

External Links
Characterization Data [Compare Characterization Data]
  Immuno-MRM
Click to enlarge image Immuno-MRM chromatogram of CPTC-UBE2I-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3261) Click image to enlarge

CPTC-UBE2I-1 IMRM

Result: Positive

Immuno-MRM chromatogram of CPTC-UBE2I-1 antibody (see CPTAC assay portal for details: https://assays.cancer.gov/CPTAC-3261)


  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-UBE2I-1 peptide ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to biotinylated peptide coated on a NeutrAvidin plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


Characterization SOP Files

  Indirect ELISA
Click to enlarge image Indirect ELISA (ie, binding of Antibody to full-length Antigen coated on plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding. Click image to enlarge

CPTC-UBE2I-1 Indirect ELISA

Result: Positive

Indirect ELISA (ie, binding of Antibody to full-length Antigen coated on plate). Note: B50% represents the concentration of Ab required to generate 50% of maximum binding.


  Western Blot - Recombinant Protein or Peptide
Click to enlarge image Western Blot using CPTC-UBE2I-1 as primary Ab against full-length recombinant Ag 10319 (lane 2). Also included are molecular wt. standards (lane 1) and the UBE2I-1 Ab as positive control (lane 3). Click image to enlarge

CPTC-UBE2I-1 Western blot

Result: Positive

Western Blot using CPTC-UBE2I-1 as primary Ab against full-length recombinant Ag 10319 (lane 2). Also included are molecular wt. standards (lane 1) and the UBE2I-1 Ab as positive control (lane 3).


Background

NCI Identification Number:

00045

Antigen Name:

Ubiquitin Conjugating Enzyme E2I Peptide 1

CPTC Name:

CPTC-UBE2I Peptide 1

Keywords:

UBC9; Ubiquitin Carrier Protein 9; Ubiquitin Carrier Protein I; Ubiquitin-Protein Ligase I; SUMO-Protein Ligase; C358B7.1; P18; SUMO-1-Protein Ligase; SUMO-Conjugating Enzyme UBC9; Ubiquitin Conjugating Enzyme 9; Ubiquitin-Conjugating Enzyme; UbcE2A; Ubiquitin-Like Protein SUMO-1 Conjugating Enzyme; Ubiquitin-Protein Ligase E2I; EC 6.3.2; UBCE9

Function:

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the
E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have
been found for this gene.

Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and
catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Can
catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear
architecture and chromosome segregation.

Chromosomal Localization:

16p13.3

NCBI Accession Number:

NP_003336.1

Swiss Prot Accession Number:

P63279

DNA Source:

N/A

Immunogen:

Synthetic Peptide

Vector Name:

N/A

Extinction Coefficient:

Buffers:

Expressed Sequence:

DHPFGFVAVPTK

Native Sequence:

Calculated Isoelectric Point:

0

Molecular Weight:

1320

Last Updated:

06/21/2013

Links

Characterization Data

SOPs

No SOPs available.

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